The present disclosure is drawn to microfluidic cellular membrane modification devices. In one example, a microfluidic cellular membrane modification device can include a microfluidic channel including a pumping portion and an electric field portion. An electrode pair can be positioned about the electric field portion. A bidirectional pump can be in fluid communication with the microfluidic channel at the pumping portion to move fluid backward and forward through the electric field portion.

A fluid delivery device comprises a hydraulic pump chamber having a hydraulic fluid. A fluid reservoir is coupled to the hydraulic pump chamber and is configured to contain a fluid deliverable to a patient. A first actuator is coupled to the hydraulic pump chamber and is configured to pressurize the hydraulic pump chamber and configured to transfer energy through the hydraulic pump chamber to the fluid reservoir. A second actuator is coupled to the hydraulic pump chamber and is configured to pressurize the hydraulic pump chamber and configured to transfer energy through the hydraulic pump chamber to the fluid reservoir.

A fluid delivery device comprises a hydraulic pump chamber having a hydraulic fluid. A fluid reservoir is coupled to the hydraulic pump chamber and is configured to contain a fluid deliverable to a patient. A first actuator is coupled to the hydraulic pump chamber and is configured to pressurize the hydraulic pump chamber and configured to transfer energy through the hydraulic pump chamber to the fluid reservoir. A second actuator is coupled to the hydraulic pump chamber and is configured to pressurize the hydraulic pump chamber and configured to transfer energy through the hydraulic pump chamber to the fluid reservoir.

A compressor assembly for further pressurizing pressurized fluid using motion of waves, the pressurized fluid being pressurized by a previous compressor assembly, is provided. The compressor assembly includes: a compression piston having a first side and a second side; a first rod portion coupled to a buoyant member coupled to the first side of the compression piston, wherein movement of the buoyant member is transferred, via the first rod portion, into movement of the compression piston between a top and bottom end of a compression chamber; and a second rod portion coupled to the second side of the compression piston and including a terminal surface, wherein the second portion is sized such that the terminal surface is located within an equalizer assembly during an entire range of motion of the compression piston. A diameter of the second portion is equal to or greater than a diameter of the first portion.

A compressor assembly for further pressurizing pressurized fluid using motion of waves, the pressurized fluid being pressurized by a previous compressor assembly, is provided. The compressor assembly includes: a compression piston having a first side and a second side; a first rod portion coupled to a buoyant member coupled to the first side of the compression piston, wherein movement of the buoyant member is transferred, via the first rod portion, into movement of the compression piston between a top and bottom end of a compression chamber; and a second rod portion coupled to the second side of the compression piston and including a terminal surface, wherein the second portion is sized such that the terminal surface is located within an equalizer assembly during an entire range of motion of the compression piston. A diameter of the second portion is equal to or greater than a diameter of the first portion.

A pump assembly. The pump assembly may comprise a motor portion for driving a fluid pumping portion via a piston. The motor portion may comprise: a spool and a spool housing assembly having a spool chamber. The spool may be disposed within the spool chamber. The spool may comprise an upper portion and a lower portion, wherein the diameter of the lower portion is larger than the diameter of the upper portion. The spool may also comprise: (1) two support groove portions for cradling two slide valves and (2) two spool bores, which are in fluid communication with the two slide valves. The piston of the pump assembly may comprise a valve stem having a valve stem bore and valve stem openings. The valve stem openings may be funnel-shaped or may inwardly slope into the valve stem bore to prevent or minimize cutting or shredding of a blown seal.

A pump assembly. The pump assembly may comprise a motor portion for driving a fluid pumping portion via a piston. The motor portion may comprise: a spool and a spool housing assembly having a spool chamber. The spool may be disposed within the spool chamber. The spool may comprise an upper portion and a lower portion, wherein the diameter of the lower portion is larger than the diameter of the upper portion. The spool may also comprise: (1) two support groove portions for cradling two slide valves and (2) two spool bores, which are in fluid communication with the two slide valves. The piston of the pump assembly may comprise a valve stem having a valve stem bore and valve stem openings. The valve stem openings may be funnel-shaped or may inwardly slope into the valve stem bore to prevent or minimize cutting or shredding of a blown seal.

At least one pressure exchange unit with a hollow cylindrical body, a piston sliding in the body, the piston including a piston head separating the interior of the cylindrical body into a downstream chamber and an upstream chamber, the downstream chamber being provided with a device for the admission and discharge of water to be treated, the upstream chamber being provided with a five-way distributor linkage including, for hydraulic balancing, two pressurized liquid supply orifices, two orifices for the evacuation of the liquid and an opening in communication with the upstream chamber.

At least one pressure exchange unit with a hollow cylindrical body, a piston sliding in the body, the piston including a piston head separating the interior of the cylindrical body into a downstream chamber and an upstream chamber, the downstream chamber being provided with a device for the admission and discharge of water to be treated, the upstream chamber being provided with a five-way distributor linkage including, for hydraulic balancing, two pressurized liquid supply orifices, two orifices for the evacuation of the liquid and an opening in communication with the upstream chamber.

The present disclosure is drawn to microfluidic cellular membrane modification. In one example, a method of modifying cells can include pumping a fluid comprising cells in a forward direction through a microfluidic channel, applying an electric field within the microfluidic channel as cells flow in the forward direction through the electric field and beyond within the microfluidic channel, and pumping the fluid in a backward direction through the microfluidic channel, wherein cells flow in the backward direction returning through the electric field.