An apparatus for microfluidic flow cytometry analysis of a particulate containing fluid An apparatus for microfluidic flow cytometry analysis of a particulate containing fluid comprises a hydrodynamic focussing apparatus for providing a focused stream of particulate containing fluid; and a microfluidic chip. The chip has a plurality of layers and comprises a microfluidic channel that extends through the chip substantially orthogonal to a plane of the layers of the chip, and is in fluid communication with the hydrodynamic focusing apparatus for receipt of a focused steam of particulate containing fluid. The chip also comprises a detection zone comprising at least one pair of electrodes in electrical communication with the microfluidic channel. At least one pair of electrodes comprise an excitation electrode coupled to an AC signal source and a detection electrode configured to detect AC impedance changes in the microfluidic channel between the electrodes resulting from particles passing between the electrodes in the microfluidic channel. Methods of sorting mammalian sperm cells according to sex is also described.

An outside opening of each aperture of a plurality of counting chambers for performing particle counting based on the electric resistance method is connected to suction pump through a confluent piping. Liquid supplying part supplies an additional liquid to the counting chamber side after completion of counting of counting chamber, so that the liquid level of sample liquid of counting chamber will not descend to aperture or a predetermined liquid level.

In the particle analyzing apparatus of the present invention, first, an inner space with a negative pressure having a predetermined volume is formed in the cylinder of a syringe device for sucking a sample liquid in the measuring chamber, then, the negative pressure is applied to the measuring chamber, the sample liquid is sucked, and measurement of particle is performed in the measuring flow path. The control device calculates a particle analysis value from the measurement signal obtained by the measurement. The particle analysis value is obtained by the sucking force of the negative pressure and the control device further corrects the particle analysis value based on a standard pressure predetermined for the inner space.

The invention relates to a nozzle for flow cytometry, the housing of which is tapering towards an outlet and in which a feed tube is arranged for a core flow liquid, the outlet opening of which is arranged at a distance from the outlet of the housing. The outlet of the housing forms the outlet of the nozzle. The housing of the nozzle extends from its outlet, which is arranged at its first end to its opposite second end, and has an inlet for a sheath flow liquid connected with the internal volume. The nozzle is characterized in that in the housing a leading element that promotes the alignment of particles extends from both sides of the feed tube.

An outside opening of each aperture of a plurality of counting chambers for performing particle counting based on the electric resistance method is connected to suction pump through a confluent piping. Liquid supplying part supplies an additional liquid to the counting chamber side after completion of counting of counting chamber, so that the liquid level of sample liquid of counting chamber will not descend to aperture or a predetermined liquid level.

Apparatuses, components, methods, and systems for interrogating samples are provided. An example system includes a flow cell. An example flow cell includes an aperture, an inlet chamber, and a sample injector positioned within the inlet chamber. An example sample injector is configured to generate a sample stream that flows in biased proximity to a profile included in the inlet chamber. An example method includes the steps of causing sheath fluid to flow into an inlet chamber and through an aperture, injecting sample into an inlet chamber with a sample injector to form a sample stream that is entrained in the sheath fluid, and interrogating the sample stream as the sample stream passes through an interrogation region within the aperture. An example sample injector includes an outlet that is disposed in an off-center position within the inlet chamber.

In the particle analyzing apparatus of the present invention, first, an inner space with a negative pressure having a predetermined volume is formed in the cylinder of a syringe device for sucking a sample liquid in the measuring chamber, then, the negative pressure is applied to the measuring chamber, the sample liquid is sucked, and measurement of particle is performed in the measuring flow path. The control device calculates a particle analysis value from the measurement signal obtained by the measurement. The particle analysis value is obtained by the sucking force of the negative pressure and the control device further corrects the particle analysis value based on a standard pressure predetermined for the inner space.

An optics-free method for single cell profiling, particularly white blood cells such as neutrophils is disclosed. Embodiments relate to multi-parametric biophysical profiling of neutrophils using a microfluidic impedance-deformability cytometry device. The device comprises a first flow channel comprising a particle focusing region, a detection region comprising a first pair of electrodes, a cell deformation zone and a second pair of electrodes. A multi-frequency impedance response of each cell before and after deformation is measured respectively by the first and second pairs of electrodes, such as to quantify membrane opacity, nucleus opacity, cell size and deformability index. A second flow channel bifurcated into two split flow pathways that converge in an intersecting manner with the first flow channel forms a cross-junction in a cell deformation zone, to hydrodynamically deform each cell by perfusing a sheath fluid along the two split flow paths from two opposite sides of the sample flow channel.

The invention relates to a nozzle for flow cytometry, the housing of which is tapering towards an outlet and in which a feed tube is arranged for a core flow liquid, the outlet opening of which is arranged at a distance from the outlet of the housing. The outlet of the housing forms the outlet of the nozzle. The housing of the nozzle extends from its outlet, which is arranged at its first end to its opposite second end, and has an inlet for a sheath flow liquid connected with the internal volume. The nozzle is characterized in that in the housing a leading element that promotes the alignment of particles extends from both sides of the feed tube.

The invention relates to a nozzle for flow cytometry, the housing of which is tapering towards an outlet and in which a feed tube is arranged for a core flow liquid, the outlet opening of which is arranged at a distance from the outlet of the housing. The outlet of the housing forms the outlet of the nozzle. The housing of the nozzle extends from its outlet, which is arranged at its first end to its opposite second end, and has an inlet for a sheath flow liquid connected with the internal volume. The nozzle is characterized in that in the housing a leading element that promotes the alignment of particles extends from both sides of the feed tube.