The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

An electronically-controlled digital ferrofluidic device is disclosed which employs a network of individually addressable coils in conjunction with one or more movable permanent magnets, where each moveable permanent magnet delivers the designated fluid manipulation-based tasks. The underlying mechanism facilitating fluidic operations is realized by addressable electromagnetic actuation of miniaturized mobile magnets that exert localized magnetic body forces on droplets filled with magnetic nanoparticles. The reconfigurable, contactless, and non-interfering magnetic-field operation properties of the underlying actuation mechanism allow for the integration of passive and active components to implement advanced and diverse operations with high efficiency (e.g., droplet sorting, dispensing, generation, merging, mixing, filtering, and analysis).

To provide an adjusting method of a positional relationship between a flow path position and a light irradiation position.

The present technology provides a position adjusting method provided with an imaging step of imaging, while moving a flow path through which a microparticle is able to flow in an optical axis direction, the flow path in a plurality of positions in the optical axis direction, a movement step of moving the flow path in the optical axis direction on the basis of a focus index for each of a plurality of images acquired at the imaging step, and an adjustment step of specifying a feature position of the flow path from an image of the flow path in a position after movement at the movement step, and adjusting a positional relationship between the feature position and a reference position in a direction perpendicular to the optical axis direction.

The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

Disclosed are devices and methods useful for confined-channel digital microfluidics that combine high-throughput droplet generators with digital microfluidic for droplet manipulation. The present disclosure also provides an off-chip sensing system for droplet tracking.

A system for processing a sample includes a chamber having at least one inlet and at least one outlet, where the chamber is configured to accommodate flow of the sample from the at least one inlet toward the at least one outlet, and an imager array configured to image the flow of the sample in the chamber, where the imager array includes at least one lensless image sensor configurable opposite at least one light source.

In various embodiments methods and devices are provided for focusing and/or sorting particles and/or cells in a microfluidic channel. In certain embodiments the device comprises a microfluidic channel comprising a plurality of electrodes disposed to provide dielectrophoretic (DEP) forces that are perpendicular to hydrodynamic flows along the channel; wherein said device is configured to apply voltages to said electrodes to provide an electric field minimum that is not centered in said microfluidic channel.

There are provided an apparatus capable of treating biological particles in a sterile state, and a treatment apparatus. An apparatus for forming a liquid flow including biological particles includes: a chamber member; a sample liquid supply portion; a sheath liquid supply portion; and a vibrating electrode member. The chamber member includes a chamber and a flow cell extending from an interior to an exterior of the chamber. The sample liquid supply portion is configured to supply a sample liquid including the biological particles into the chamber. The sheath liquid supply portion is configured to supply a sheath liquid into the chamber. The vibrating electrode member extends from the interior to the exterior of the chamber, is made of an electrically conductive material, and is configured to be capable of supplying an electric charge to the sheath liquid and the sample liquid in the chamber and propagating an ultrasonic vibration.

Disclosed are devices and methods useful for confined-channel digital microfluidics that combine high-throughput droplet generators with digital microfluidic for droplet manipulation. The present disclosure also provides an off-chip sensing system for droplet tracking.