The present invention relates to vitro method for analysing a liquid sample as to the presence, identity and properties of microbes comprising: a) isolating microbes from the liquid sample; b) analysing said microbes spectroscopically by means of spontaneous Raman spectroscopy; and c) determining antibiotic susceptibility of said microbes spectroscopically by means of spontaneous Raman spectroscopy. The present invention also refers to device for analysing a liquid sample as to the presence, identity and properties of microbes, wherein the device comprises as a first unit (i) a chip comprising a filtering unit and an antibiotics exposure unit capable of determining the susceptibility of microbes to an antibiotic; as a second unit (ii) a Raman spectroscopy system; and as a third unit (iii) an evaluation module which is coupled to the Raman spectroscopy system.

A biological sample reaction vessel comprising a reagent storage portion and a push rod movable relative to the reagent storage portion is provided. The reagent storage portion comprises at least one reagent containing cavity, and the reagent containing cavity is sealed by a sealing element; and the push rod is connected to the sealing element, and the push rod is used for cooperation with an external device to separate the sealing element from the reagent storage portion. In reaction, the biological sample reaction vessel cooperates with a test cassette. By inserting the biological sample reaction vessel into the external device, the reagent in the reagent storage portion can be released rapidly.

The present invention concerns a multilayer structure for a biosensor, comprising a base layer, a biocompatible layer comprising a reagent on the base layer, a self-adhesive layer on the biocompatible layer, such that the reagent is at least partially aligned with a channel formed in the self-adhesive layer, and a top layer on the self-adhesive layer. According to the present invention, the biocompatible layer is deposited directly onto the base layer and is adhesive. The present invention also concerns a biosensor and a method for the manufacture of such a multilayer structure.

The present disclosure provides a microfluidic chip, including: first base substrate and a second base substrate opposite to each other; first electrode and second electrode between the first base substrate and the second base substrate and configured to control droplet to move between the first base substrate and the second base substrate according to voltages applied on the first electrode and the second electrode; light guide component configured to guide light propagating in the first base substrate to the droplet; shading component and detection component, shading component having light transmission regions spaced from each other, light transmission regions being configured to transmit light passing through the droplet to the detection component, wherein detection component is on second base substrate and is configured to obtain property of the droplet according to an intensity of the light passing through droplet and received from the light transmission regions.

An optical cell for performing light spectroscopy (including absorbance, fluorescence and scattering measurements) on a liquid sample in microfluidic devices is disclosed. The optical cell comprises an inlaid sheet having an opaque material inlaid in a clear material, and a sensing channel that crosses the clear material and the opaque material provides a fluidic path for the liquid sample and an optical path for probe light. Integral optical windows crossing a clear-opaque material interface permit light coupling into and out of the sensing channel, and thus light transmission through the sensing channel is almost entirely isolated from background light interference. A microfluidic chip comprising one or more optical cells is also disclosed. The optical cells may have different lengths of sensing channels, and may be optically and fluidly coupled. A method of manufacturing an optical cell in a microfluidic chip is also disclosed.

A microfluidic channel structure and a fabrication method thereof, a microfluidic detecting device and a detecting method thereof are disclosed. The microfluidic channel structure includes a support portion; a foundation portion, provided on the support portion and including a first foundation and a second foundation spaced apart from each other; and a channel defining portion, provided on a side of the foundation portion that is away from the support portion and including a first channel layer and a second channel layer, the first channel layer covering the first foundation and the second channel layer covering the second foundation have a gap therebetween to define a microfluidic channel; and the first channel layer and the second channel layer are made of a same material.

Flowcell devices configured for reversible assembly, and methods of assembly, disassembly, and use thereof are provided. The methods and devices allow the interior of the flowcell device to be accessed without damaging, or otherwise disturbing sensitive samples and surfaces.

An optical cell for performing light spectroscopy (including absorbance, fluorescence and scattering measurements) on a liquid sample in microfluidic devices is disclosed. The optical cell comprises an inlaid sheet having an opaque material inlaid in a clear material, and a sensing channel that crosses the clear material and the opaque material provides a fluidic path for the liquid sample and an optical path for probe light. Integral optical windows crossing a clear-opaque material interface permit light coupling into and out of the sensing channel, and thus light transmission through the sensing channel is almost entirely isolated from background light interference. A microfluidic chip comprising one or more optical cells is also disclosed. The optical cells may have different lengths of sensing channels, and may be optically and fluidly coupled. A method of manufacturing an optical cell in a microfluidic chip is also disclosed.

An assay device as well as a method of use thereof is described. The assay device includes a planar substrate having a top surface and a bottom surface. The assay device further includes one or more flow channels disposed within the planar substrate and extending along a dimension of the planar substrate between the top surface and the bottom surface. The assay device further includes an inlet fluidly coupled to the one or more flow channels and one or more vents fluidly coupled to the one or more channels which are operable to facilitate flow of a liquid sample, such as whole blood through the one or more channels. The one or more flow channels are configured to receive a liquid sample from the inlet and allow flow of the liquid sample.

An optical cell for performing light spectroscopy (including absorbance, fluorescence and scattering measurements) on a liquid sample in microfluidic devices is disclosed. The optical cell comprises an inlaid sheet having an opaque material inlaid in a clear material, and a sensing channel that crosses the clear material and the opaque material provides a fluidic path for the liquid sample and an optical path for probe light. Integral optical windows crossing a clear-opaque material interface permit light coupling into and out of the sensing channel, and thus light transmission through the sensing channel is almost entirely isolated from background light interference. A microfluidic chip comprising one or more optical cells is also disclosed. The optical cells may have different lengths of sensing channels, and may be optically and fluidly coupled. A method of manufacturing an optical cell in a microfluidic chip is also disclosed.