The invention describes an iterative reconstructing method allowing a spatial distribution of fluorescence in an object to be obtained. The method comprises acquiring images of fluorescence in various planes at various depths in the object, so as to form a three-dimensional acquired image. It comprises an iterative reconstructing algorithm with, in each iteration, an initial fluorescence distribution or a fluorescence distribution resulting from a preceding iteration being taken into account, and the fluorescence light wave propagating through the object being simulated, so as to obtain a reconstruction of the acquired image. The acquired image, or a differential image corresponding to a comparison between the acquired image and the reconstructed image, is then back-propagated through the object, so as to update the fluorescence distribution. FIG. 5B.

Systems and methods for producing background-reduced fluorescence imaging signals include an illumination system that provides illumination light from an illumination source to a targeted area on the sample platform, a sensor adapted to detect light and having an array of sensing locations, and collection optics arranged and configured to project light emanating from the sample platform onto the sensor. In typical operation, light from the targeted area is projected onto a first portion of the sensor comprising a first plurality of the sensing locations and light from proximal to the targeted area on the platform is projected onto a second portion of the sensor comprising a second plurality of the sensing locations, and a second signal detected by the second portion of the sensor is subtracted from a first signal detected by the first portion of the sensor to produce a background-reduced signal, e.g., a signal with reduced background related noise.

An inspection method, correction method, and inspection device that include measuring a first spatial position where laser light is emitted at a first region and measuring a first strike position where the inspection device is struck by the laser light in the first region, the measurements being performed by emitting the laser light at the first region of the inspection device; measuring a second spatial position where the laser light is emitted at a second region and measuring a second strike position where the inspection device is struck by the laser light in the second region, the measurements being performed by emitting the laser light at the second region of the inspection device; and comparing measurement results for the first spatial position and the second spatial position with measurement results for the first strike position and the second strike position.

The invention relates to a method for determining an averaged color value of a transparent bulk material, which allows an online measurement of the averaged color value in transmission. Also disclosed is a sample of a transparent bulk material having an averaged color value with small standard deviation and a molded body which comprises such a sample.

A microscopic technique for generating high-clarity, large volume 3D images of fluorescent tissue structure at subcellular resolution and capture transient activities. The technique includes capturing two orthogonal 2D projection of the sample volume by performing a projection scan with an excitation laser sweeping through the volume at up to 100 vps, tracking the scan depth using an electrically tuned lens to keep the emission image in focus and generate an xy plane volume projection image at the camera; and placing a PMT behind the excitation lens to collect emission passed through the excitation lens, wherein signals from the PMT form a focus scan projection at the yz plane; and then merging the xy and yz projections.

A standoff chemical detection system that includes a source and detector are provided. The source includes: a controller, memory communicatively connected to the controller, optical sources each constructed to operate over different wavelength ranges, and a power supply. The controller controls the plurality of optical sources to emit respective infrared beams towards a target detection area in a sequential order. The detector includes: an image sensor and a controller that is communicatively connected to the image sensor. Memory and the notification device are also communicatively connected to the controller. The image sensor receives attenuated infrared beams emitted by the optical sources sequentially and at least partially attenuated by chemicals in the target detection area. The controller is constructed to calculate stimulus value signals from the recorded image data and determine whether a hazard chemical is located within the target detection area based on the calculated stimulus value signals.

A metrology three-dimensional (3D) scanning system includes a metrology 3D scanning application (app) comprising computing instructions that, when executed by one or more processors, causing the one or more processors to: record human-robot interaction (HRI) data as a human operator operates the HRI device; generate a preliminary scan path based on the HRI data for operating a robotic element within an operating environment; move the robotic element along at least a portion of the preliminary scan path and record preliminary scan data comprising at least a subset of dimension data defining at least a target object; generate a metrology scanning path plan and a motion plan for the robotic element based on the preliminary scan data; and execute instructions to move the robotic element within the operating environment according to the metrology scanning path plan and the motion plan for scanning the target object.

An imaging system may include an imaging metrology tool with an illumination source, one or more illumination optics to direct illumination from the illumination source to a sample, a detector, one or more collection optics to image the sample onto the detector; and one or more aberration-controlling components. The one or more aberration-controlling components may provide aberration correction for imaging the sample onto the detector according to one or more degrees of freedom, where the one or more degrees of freedom include at least a defocus of the imaging system, and where the one or more aberration-controlling components are integrated with at least one of the one or more illumination optics, the one or more collection optics, or the detector.

Near InfraRed NIR technology, including NIR cameras and detectors, is used to accurately identify surface irregularities on a veneer surface. A grade is then assigned to the veneer based, at least in part, on the detected irregularities. In one embodiment, the veneer is then provided to an improved veneer stacking system that produces more consistently graded veneer stacks and safer veneer stacks, is less expensive to operate, and is far safer than currently available methods and systems for full veneer sheet, veneer strip, and partial veneer sheet stacking.

Near InfraRed NIR technology, including NIR cameras and detectors, is used to accurately identify surface irregularities on a veneer surface. A grade is then assigned to the veneer based, at least in part, on the detected irregularities. In one embodiment, the veneer is then provided to an improved veneer stacking system that produces more consistently graded veneer stacks and safer veneer stacks, is less expensive to operate, and is far safer than currently available methods and systems for full veneer sheet, veneer strip, and partial veneer sheet stacking.