A biological sensing apparatus includes an optical waveguide substrate, a surface plasmon resonance (SPR) layer, and a lossy mode resonance (LMR) layer. The optical waveguide substrate includes a light input end and a light output end opposite to each other, and a biological sensing area is formed on one surface of the optical waveguide substrate between the light input end and the light output end. The SPR layer includes a metal layer and a plurality of biological probes. The metal layer is arranged on part of the biological sensing area, and the plurality of biological probes are evenly arranged on the metal layer. The LMR layer is arranged on part of the biological sensing area, and the LMR layer and the SPR layer are not overlapped. The present disclosure further includes a biological sensing system and a method of using the same.

A method for preparing fluorescent-encoded microspheres coated with metal nanoshells is disclosed herein. By using SPG method, metal nano-material modified with a certain ligand is used as a new surfactant in the emulsification process, and different kinds and different amounts of fluorescent materials are doped into polymer microspheres to prepare fluorescent-encoded microspheres with different fluorescent-encoded signals and uniformly coated metal nanoshells in one step. The prepared fluorescent-encoded microsphere comprises a metal nanoshell, a polymer, and a fluorescent-encoded material. The fluorescent-encoded microsphere has a particle size of 1 μm˜20 μm, CV of less than 10%, which can be used for protein/nucleic acid detection. The preparation method has the advantages of simple process, high surface coating rate, good uniformity and controllable LSPR peaks, which can solve the problems of existing commonly used metal nanoshell coating methods such as low surface coating rate, poor uniformity, complex preparation process and uncontrollable local surface plasmon resonance (LSPR) peaks, etc.

A process for sizing two-dimensional nanostructures includes providing the nanostructures to a liquid-liquid interface, providing probe particles to the liquid-liquid interface, obtaining an image of the nanostructures and the probe particles, and processing the image to ascertain a size property of the nanostructures.

A measurement system for obtaining information about a sample comprises an excitation-beam source configured for irradiating the sample with an excitation-beam. The measurement system comprises a probe unit configured for exposing the sample to a probing radiation or a probing field, and a detection unit configured for obtaining a first information about an interaction of the probing radiation or the probing field with the sample, if a plasmon or plasmon-polariton was excited by the excitation-beam, and obtaining a second information about an interaction of the probing radiation of the probing field with the sample, if a plasmon or plasmon-polariton was not excited by the excitation-beam.

There is provided a microfluidic chip for sensing an analyte in a sample by colorimetry. The microfluidic chip comprises: an inlet adapted to receive the sample; an incubation chamber having an incubation chamber inlet fluidly connected to the inlet downstream thereof, to incubate the analyte in the sample; a filter barrier fluidly connected to the incubation chamber, downstream of the incubation chamber inlet; a sensing chamber fluidly connected to the incubation chamber, downstream of the filter barrier, the sensing chamber having a plasmonic nanosurface, the plasmonic nanosurface including nanostructures protruding from the plasmonic nanosurface, the nanostructures having a size that is smaller than that of the diffraction limit of light, the nanostructures having a metallic layer that is plasmon-supported on top of a back reflector layer; and an outlet fluidly connected to the sensing chamber downstream thereof.

A biological sensing apparatus includes an optical waveguide substrate, a surface plasmon resonance (SPR) layer, and a lossy mode resonance (LMR) layer. The optical waveguide substrate includes a light input end and a light output end opposite to each other, and a biological sensing area is formed on one surface of the optical waveguide substrate between the light input end and the light output end. The SPR layer includes a metal layer and a plurality of biological probes. The metal layer is arranged on part of the biological sensing area, and the plurality of biological probes are evenly arranged on the metal layer. The LMR layer is arranged on part of the biological sensing area, and the LMR layer and the SPR layer are not overlapped. The present disclosure further includes a biological sensing system and a method of using the same.

The invention discloses a biosensor device (100) to detect the presence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection in a biological sample. The device includes an optical fiber probe (104) having a curved portion (104a) with a probe region (105) immobilized with bioreceptor molecules (201) configured to bind to the target molecule V indicative of the presence of the SARS-CoV-2. The probe has a light source (102) and a detector (106) on either end. The device works on the principle of plasmonic fiberoptic absorbance biosensing. Plasmonic gold nanoparticles (120) are used as either sensor substrate over the fiber or labels conjugated with a biorecognition molecule (211). The probe is exposed to a biological sample either directly for label-free detection, or after mixing with labels to realize a sandwich assay. The target biomolecules are detected by a proportional drop in the light intensity passing through the probe.

Disclosed is a method for classifying monitoring results from an analytical sensor system (20) arranged to monitor molecular interactions at a sensing surface, wherein detection curves representing progress of the molecular interactions with time are produced. The method comprises steps of: acquiring (100) a set of detection curves, fitting (101) a first mathemati- cal model to the set of detection curves; calculating (102) a set of features from the set of detection curves and fitted mathematical model; based on the calculated set of features, classifying (103) each detection curve into qual- ity classification group; and based on the classification determining which detection curves to use in kinetic analysis of the monitored molecular inter- actions.

Manufacturing a device may include inspecting a surface of an inspection target device. The inspecting may include forming a metal layer on a surface of the inspection target device on which a minute pattern is formed, directing a beam of light to be incident and normal to the surface of the inspection target device, determining a spectrum of light reflected from the surface of the inspection target device, and generating, via the spectrum, information associated with a structural characteristic of the minute pattern formed on the inspection target device. The inspection target device may be selectively incorporated into the manufactured device based on the generated information.

The present invention provides a method for studying transport of an agent across a membrane comprising the steps a) providing at least one surface with a bilayer structure tethered to the surface, said bilayer structure comprising a detection volume, b) contacting the bilayer with at least one agent to be analyzed, and c) detecting a change in refractive index in the detection volume resulting from transportation of the agent across the membrane. Further there is provided a device comprising a) at least one surface, b) at least one bilayer structure tethered to the surface, and c) at least one sensor capable of detecting a change in refractive index in a detection volume, wherein the bilayer structure encloses a first volume of the detection volume and wherein the volume not enclosed by the bilayer structure but within the detection volume is a second volume and wherein the ratio between the first volume and second volume is above about 0.001.