Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

A single chirality single walled carbon nanotubes (SWNT), and combinations thereof, can be used to detect trace levels of chemical compounds in vivo with high selectivity.

Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

A time measuring apparatus 10 includes a digital counter 20 that outputs a count signal in response to a clock signal, a plurality of TAC circuits 12 (TAC circuits 12a to 12j) to which a detection signal detected by a detector 4 and a clock signal are input and which output measurement signals corresponding to a time between the detection signal and the clock signal, a control unit 14 that derives and outputs time information related to the detection signal based on the count signal output from the digital counter 20 and the measurement signals output from the TAC circuits 12, and a measurement gate 11 that switches between the TAC circuits 12 to which the detection signal is input, in consideration of dead times of the TAC circuits 12.

Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

A femtosecond laser multimodality molecular imaging system includes a near-infrared pulse generation device for providing near-infrared pulses with a central wavelength of 1010 nm to 1100 nm and a spectral width of less than 25 nm. The near-infrared pulses can excite an optical medium with strong nonlinearity to generate the femtosecond laser pulses with ultra-wide spectrum. A pulse measurement compression and control module measures and compensates the accumulated dispersion of the femtosecond laser pulses arriving at the tissue sample, so as to eliminate the “time domain broadening” effect as much as possible. The obtained shortest pulses can interact with the tissue sample to generate spectral signals from different modalities, thus providing a variety of nonlinear molecular image modalities.

A single chirality single walled carbon nanotubes (SWNT), and combinations thereof, can be used to detect trace levels of chemical compounds in vivo with high selectivity.

A femtosecond laser multimodality molecular imaging system includes a near-infrared pulse generation device for providing near-infrared pulses with a central wavelength of 1010 nm to 1100 nm and a spectral width of less than 25 nm. The near-infrared pulses can excite an optical medium with strong nonlinearity to generate the femtosecond laser pulses with ultra-wide spectrum. A pulse measurement compression and control module measures and compensates the accumulated dispersion of the femtosecond laser pulses arriving at the tissue sample, so as to eliminate the time domain broadening effect as much as possible. The obtained shortest pulses can interact with the tissue sample to generate spectral signals from different modalities, thus providing a variety of nonlinear molecular image modalities.

An improved DNA sequencing system comprising a DNA sample holder residing on a stacked BSI global shutter image sensor illuminated by a pulsed laser for fluorescent illumination detection. The pulsed laser has on and off periods wherein during the laser on period a Fluorophore tag attached to a DNA sample is excited to produce fluorescence emission while the imaging system captures no illumination and during the off period the global shutter imaging system captures persistent fluorescent emission from the DNA sample and reads out an imaging signal.

Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.