Methods, systems and devices that allow independently applied pressures to a BGE reservoir and a sample reservoir for pressure-driven injection that can inject a discrete sample plug into a separation channel that does not require voltage applied to the sample reservoir and can allow for in-channel focusing methods to be used. The methods, systems and devices are particularly suitable for use with a mass spectrometer.

A method for separating diastereomers of pristane. A pristane sample is prepared, and then injected into a chromatographic instrument equipped with a chiral chromatographic column, where a stationary phase of the chiral chromatographic column has a preset pore size. The pristane diastereomers in the pristane sample are separated by the chiral chromatographic column, and the components produced by the separation of the pristane diastereomers sequentially enter a mass spectrometer for detection and analysis.

A method for separating diastereomers of pristane. A pristane sample is prepared, and then injected into a chromatographic instrument equipped with a chiral chromatographic column, where a stationary phase of the chiral chromatographic column has a preset pore size. The pristane diastereomers in the pristane sample are separated by the chiral chromatographic column, and the components produced by the separation of the pristane diastereomers sequentially enter a mass spectrometer for detection and analysis.

A column device for an automatic analyser. The automatic analyzer comprises a high performance liquid chromatography (HPLC) module. The HPLC module comprises a fixation device configured to automatically fix and release a chromatographic column. The column device comprises a column jacket and a capillary. The capillary comprises predetermined dimensions and is disposed within the column jacket. The column device is configured to be installed at the HPLC module using the fixation device. Further, an automatic analyzer is disclosed.

Methods, systems and devices that allow independently applied pressures to a BGE reservoir and a sample reservoir for pressure-driven injection that can inject a discrete sample plug into a separation channel that does not require voltage applied to the sample reservoir and can allow for in-channel focusing methods to be used. The methods, systems and devices are particularly suitable for use with a mass spectrometer.

In gas chromatography (GC), a sample is introduced into a flow of carrier gas and the mixture is driven through a heated GC column to acquire chromatographic data from the sample. During this time, the column is heated from an initial temperature to a final temperature. Subsequently, the column is cooled according to a cooling program. The cooling program may include a first cooling ramp, a subsequent isothermal hold, and a subsequent second cooling ramp. Alternatively, while the column is cooled down the flow of carrier gas through the column may be slowed down or ceased for a period of time, after which the flow of carrier gas through the column may be resumed at the original flow rate in preparation for processing another sample. Controlling column temperature and/or flow in this manner may be effective for reducing column bleed carryover and/or the effects thereof.

A method comprising: introducing a sample volume into an inlet end of a liquid chromatography column, wherein the liquid chromatography column includes a focusing segment proximal to the inlet end of the liquid chromatography column and a separation segment proximal to an elute outlet of the liquid chromatography column; maintaining only the focusing segment at a first temperature as the sample is introduced into the focusing segment; and subsequently heating the focusing segment to a second temperature that is higher than the first temperature after the entire sample volume has been introduced into the focusing segment.

A method of comprising: introducing a sample volume into an inlet end of a liquid chromatography column, wherein the liquid chromatography column includes a focusing segment proximal to the inlet end of the liquid chromatography column and a separation segment proximal to an elute outlet of the liquid chromatography column; maintaining only the focusing segment at a first temperature as the sample is introduced into the focusing segment; and subsequently heating the focusing segment to a second temperature that is higher than the first temperature after the entire sample volume has been introduced into the focusing segment.

Methods that allow independently applied pressures to a BGE reservoir and a sample reservoir for pressure-driven injection that can inject a discrete sample plug into a separation channel that does not require voltage applied to the sample reservoir and can allow for in-channel focusing methods to be used. The methods are particularly suitable for use with a mass spectrometer.

In gas chromatography (GC), a sample is introduced into a flow of carrier gas and the mixture is driven through a heated GC column to acquire chromatographic data from the sample. During this time, the column is heated from an initial temperature to a final temperature. Subsequently, the column is cooled according to a cooling program. The cooling program may include a first cooling ramp, a subsequent isothermal hold, and a subsequent second cooling ramp. Alternatively, while the column is cooled down the flow of carrier gas through the column may be slowed down or ceased for a period of time, after which the flow of carrier gas through the column may be resumed at the original flow rate in preparation for processing another sample. Controlling column temperature and/or flow in this manner may be effective for reducing column bleed carryover and/or the effects thereof.