A sensor module includes a sensor configured to detect a specific substance in a sample, a first channel, and a second channel The first channel supplies a first fluid as the sample to the sensor. The second channel supplies a second fluid different from the first fluid to the sensor. The second channel includes a second fluid buffer tank for holding the second fluid for a fixed time interval.

The exemplary embodiments may provide liquid chromatography systems that are smaller in size and with reduced extra-column volume than conventional liquid chromatography systems. The exemplary embodiments may reduce the size of the liquid chromatography systems enough that the liquid chromatography systems of the exemplary embodiments may be deployed adjacent to automated sample preparation robotics, adjacent to a process stream, or adjacent to the inlet of a mass spectrometer. In addition, the exemplary embodiments may reduce the extra-column volume of the liquid chromatography system by eliminating many of the connection tubes found in conventional liquid chromatography systems and by situating components of the liquid chromatography system in closer proximity.

Provided is a gas chromatograph device capable of appropriately determining that a temperature of an entirety of a heating target has stabilized after cooling the heating target in a column oven to a target temperature after a temperature-rising analysis. A gas chromatograph device capable of performing a temperature-rising analysis cools an inside of a column oven by a cooling mechanism. When a detection temperature by a temperature sensor for detecting a temperature of the heating target has reached a target temperature after the inside of the column oven has been cooled, the heating target is heated by a heater. Upon reaching of the detection temperature of the temperature sensor to the target temperature, it is determined whether or not the temperature of the entirety of the heating target has stabilized based on power consumption of the heater.

Apparatus and methods for performing chromatography may include a chromatography column and a vacuum insulated jacket having an inner wall and an outer wall. A vacuum area may be formed between the inner wall and the outer wall. The inner wall of the vacuum insulated jacket may surround the chromatography column. A gap may be formed between an outer wall of the chromatography column and the inner wall of the vacuum insulated jacket. The vacuum insulated jacket may extend beyond one or more end frits of the column. The gap may be filled with one or more materials so as to form an insulating or thermal barrier.

A thermal impact assembly for a sample separation apparatus for separating a fluidic sample in a mobile phase by a sample separation unit includes a thermal impact device configured for thermally impacting the fluidic sample and/or the mobile phase and the sample separation unit, and a control unit configured for controlling the thermal impact device for thermally impacting the fluidic sample and/or the mobile phase on the one hand and for thermally impacting the sample separation unit on the other hand independently from each other.

Exemplary embodiments eliminate the need for column ovens in serial column chromatography arrangements and systems by using insulated sleeves. The insulated sleeves may encase individual chromatography columns or clusters of chromatography columns. The use of the insulated sleeves allows the chromatography columns to be positioned in close proximity to each other. This may decrease the overall size of a serial column chromatography arrangement and may reduce costs by not requiring the column ovens.

Exemplary embodiments may compensate for expected frictional heating or Joule-Thomson cooling in chromatography columns. Frictional heating or Joule Thomson cooling are the same thing for a fluid decompressing along a porous material. Either heat is absorbed from or released to the external environment. The exemplary embodiments may cool the mobile phase to a sub-ambient temperature before the mobile phase passes through a chromatography column to compensate for the frictional heating or heat the mobile phase to a super-ambient temperature to compensate for Joule-Thomson cooling. The amount of temperature increase expected from the frictional heating or the amount of temperature decrease expected from the Joule-Thomson cooling may be calculated or estimated. Based on the amount of temperature increase or decrease expected, the set point for the heater/cooler may be determined and applied to the mobile phase. The analyte may be injected solely into a central portion of the chromatography column to further compensate for thermal gradients.

Techniques are described for accelerating thermal equilibrium in a chromatographic column. An apparatus comprises a chromatography column, and a plurality of temperature control units in thermal contact with the chromatography column. A method of performing liquid chromatography comprises setting an inlet of a chromatography column to a first temperature using a first temperature control unit in thermal contact with said inlet, setting an outlet of the chromatography column to a second temperature using a second temperature control unit in thermal contact with the outlet, wherein the first temperature is less than the second temperature; and injecting a sample into a liquid stream that flows through the chromatography column after the inlet is set at the first temperature and the outlet is at the second temperature.

Apparatus and methods for performing chromatography may include a chromatography column and a vacuum insulated jacket having an inner wall and an outer wall. A vacuum area may be formed between the inner wall and the outer wall. The inner wall of the vacuum insulated jacket may surround the chromatography column. A gap may be formed between an outer wall of the chromatography column and the inner wall of the vacuum insulated jacket. The vacuum insulated jacket may extend beyond one or more end frits of the column. The gap may be filled with one or more materials so as to form an insulating or thermal barrier.

Provided herein, inter alia, are compositions, systems, and methods for the purification of granulocyte colony-stimulating factor (GCSF). Also provided are GCSF obtained by the disclosed methods and systems, pharmaceutical compositions containing the same, as well as methods for the treatment and/or prevention of a disease or health condition in an individual in need thereof.