Described is a mixer for a chromatography system. The mixer includes an inlet manifold channel, an outlet manifold channel and a plurality of transfer channels. The inlet manifold channel has an inlet at a proximal end of the inlet manifold channel for receiving an inlet flow. The transfer channels are fluidly connected between the inlet and outlet manifold channels. The respective fluid connections are distributed along each of the inlet and outlet manifolds channels. The transfer channels have different volumes. The mixer may be formed of a plurality of layer and the layers may be diffusion bonded to each other.

A system for blending solutions and a buffer solution is disclosed. In this system a switch valve is present capable of flowing one or more solutions, a low pressure pump for pumping the one or more solutions through the switch valve and a T-joint capable of receiving the one or more solutions through the low pressure pump and blending the one or more solutions with a buffer solution. A high pressure pump is present for collecting a blended solution.

A chromatography system comprising at least two pumps, a first pump which is connectable or connected with a liquid reservoir for a first fluid, and a second pump which is connectable or connected with a liquid reservoir for a second fluid, wherein the pump outlet lines from the first pump and the second pump are connected with a connection piece and, viewed in the direction of flow, a chromatography column is provided downstream of this connection piece, wherein, viewed in the direction of flow, an addition unit is provided upstream of the connection piece and a mixer switching valve and a mixer switchable by way of the mixer switching valve are provided between the connection piece and chromatography column, wherein the mixer switching valve has at least two switching positions, wherein the mixer is connectable in a first position and the mixer is bypassable in a second position.

A chromatography method in which the system is used and a conversion kit for converting a high-performance liquid chromatography system into a chromatography system for supercritical fluid chromatography are also disclosed.

Method for adapting the concentration of a sample gas in a gas mixture to be analysed by a gas chromatograph assembly (10), the gas chromatograph assembly (10) comprising a sample gas inlet (20) for introducing a sample gas to be analysed, a secondary gas inlet (40), a gas chromatograph infrared sensor (12), a gas chromatograph column (26), and a gas chromatograph bypass (28) parallel to the column (26), characterized by a) introducing an amount of sample gas through the sample gas inlet (20), b) introducing an amount of secondary gas through the secondary gas inlet (40), c) mixing the sample gas and the secondary gas to a gas mixture and conducting the gas mixture via the gas chromatograph bypass (28), d) circulating the gas mixture in a gas conducting loop (52) comprising the gas chromatograph bypass (28), the gas chromatograph infrared sensor (12) and not comprising the gas chromatograph column (26), e) analysing the gas mixture thus obtained by means of gas chromatography employing the gas chromatograph column (26) and the gas chromatograph infrared sensor (12).

Method for adapting the concentration of a sample gas in a gas mixture to be analysed by a gas chromatograph assembly (10), the gas chromatograph assembly (10) comprising a sample gas inlet (20) for introducing a sample gas to be analysed, a secondary gas inlet (40), a gas chromatograph infrared sensor (12), a gas chromatograph column (26), and a gas chromatograph bypass (28) parallel to the column (26), characterized by a) introducing an amount of sample gas through the sample gas inlet (20), b) introducing an amount of secondary gas through the secondary gas inlet (40), c) mixing the sample gas and the secondary gas to a gas mixture and conducting the gas mixture via the gas chromatograph bypass (28), d) circulating the gas mixture in a gas conducting loop (52) comprising the gas chromatograph bypass (28), the gas chromatograph infrared sensor (12) and not comprising the gas chromatograph column (26), e) analysing the gas mixture thus obtained by means of gas chromatography employing the gas chromatograph column (26) and the gas chromatograph infrared sensor (12).

A microfluidic device and a method for mixing liquids by moving the liquids back-and-forth between a chamber of a piston pump and a cavity of a spectrophotometric measuring cell. The disclosure also relates to physicochemical analysis of a mixture directly within the microfluidic device wherein the mixture is obtained using the method described herein. The disclosure also relates to a device and a method for sampling liquids remotely.

A bioprocess fluid mixing system (3; 3′; 3″; 103; 103′), said fluid mixing system (3; 3′; 3″; 103; 103′) comprising: —at least two fluid inlets (5a, 5b, 5c, 5d, 5e), configured for providing a first fluid into the fluid mixing system through a first fluid inlet (5a) and for providing a second fluid into the fluid mixing system through a second fluid inlet (5b); —at least one valve arrangement (13a, 13b, 13c, 13a′), where a first valve arrangement (13a; 13a′) is in fluid communication with at least both the first fluid inlet (5a) and the second fluid inlet (5b); —at least two pumps (11a, 11b, 11c, 11d, 11e), where a first pump (11a) is in selective fluid communication with at least both the first and the second fluid inlets (5a, 5b) via the first valve arrangement (13a; 13a′) and a second pump (11b) is in fluid communication with at least one of the first and second fluid inlet (5b); and —a common fluid outlet (14) which is in fluid communication with both an outlet (15a) of the first pump (11a) and an outlet (15b) of the second pump (11b), wherein pump rates of the at least two pumps (11a, 11b) and valve positions in the at least one valve arrangement (13a; 13a′; 13b, 13c) are configured to be controllable by a control system (21) such that mixing of at least a first fluid from the first fluid inlet (5a) and a second fluid from the second fluid inlet (5b) can be performed to a requested mixing of the at least two fluids and to a requested combined fluid flow rate at the common fluid outlet (14).

When a liquid in the column is replaced by carbon dioxide in a supercritical state in the chromatograph, an operation of a first pump is controlled by a flow rate control unit, and the carbon dioxide in the supercritical state is supplied at a constant pressure. Moreover, when a flow rate of the carbon dioxide in the supercritical state reaches a predetermined flow rate thereafter, the flow rate control unit controls an operation of the first pump so that the carbon dioxide in the supercritical state is supplied at a constant flow rate.

Described is a mixer for a liquid chromatography system. The mixer includes an inlet, an outlet, a first flow channel, and a second flow channel. The inlet receives a fluid flow to be mixed and the outlet provides the mixed fluid flow. Each of the two flow channels is coupled between the inlet and the outlet. The second flow channel includes an offset volume that delays fluid propagation through the second flow channel relative to the first flow channel. The offset volume includes a coiled channel which increases radial dispersion and decreases axial dispersion of a fluid flowing through the offset volume, thereby enabling a further reduction in periodic noise in a detector baseline signal as compared to known split flow mixers for liquid chromatography systems.

A first mixer mixes solvents therein. A second mixer has a capacity different from that of the first mixer, and mixes solvents therein. A first separation column is associated with the first mixer. A second separation column is associated with the second mixer. A first valve enables switchover between a first communication state in which the first mixer and the first separation column communicate with a detector, and a second communication state in which the second mixer and the second separation column communicate with the detector. Only by switching the first valve, it is possible to switch between the first communication state and the second communication state. The internal capacity of a flow channel in the first communication state differs from that in the second communication state. Therefore, it is easy to perform analysis with different internal capacities.