Provided are a spectral calibration apparatus and a spectral calibration method capable of performing spectral calibration with high accuracy even when peaks appear simultaneously between fluorescent dyes. The spectral calibration apparatus for calculating a color conversion matrix used in color conversion processing, includes a spectral signal acquisition unit that acquires a spectral signal of fluorescence detected over time, a candidate calculation unit that calculates a candidate of the color conversion matrix for each value of a parameter, which depends on a frequency at which fluorescence peaks of fluorescent dyes appear at the same time, based on the spectral signal, and a selection unit that selects a color conversion matrix based on an evaluation value calculated for each candidate.

The concentration measurement device 100 includes an electric unit 20 having a light source 22 and a photodetector 24, a fluid unit 10 having a measurement cell 1, optical fibers 11 and 12 for connecting the electric unit 20 and the fluid unit 10 and is configured to measure the concentration of the fluid in the measurement cell by detecting the light incident from the light source 22 to the measurement cell and then emitted from the measurement cell by the photodetector 24, where optical connection parts 32 and 34 connected to the optical fibers 11, 12 and the light source 22 or the photodetector 24 are integrally provided in the electric unit 20.

Devices, systems, and methods described herein relate to determining a concentration of a species of interest in a sample by using a spectrometer. For example, a concentration of a species of interest may be determined by passing a first feed of a sample with a species of interest through a flow-through variable pathlength spectrophotometer and reading a first absorbance value. A change in the concentration of the species of interest may be effected in the sample, and a second feed of the sample may be passed through a flow through variable pathlength spectrophotometer. A second absorbance value may be read. The difference between the first absorbance value and the second absorbance value may be used to determine the concentration of the species of interest.

Biosensor including an array of reaction sites and corresponding light sensors may experience crosstalk in which photons from one reaction site are detected by neighbors of its corresponding light sensor, and such crosstalk may be corrected using sharpening kernels corresponding to the sensors in the array. Such sharpening kernels may be derived from generative matrices, which themselves may be derived from point spread functions representing dispersion of illumination emitted from the reaction sites.

When a measurement sample whose absorbance greatly changes depending on a wavelength range is measured, measurement with a high S/N ratio and accuracy can be efficiently performed in a short time.

For a plurality of wavelength ranges in wavelength scanning measurement of a measurement sample, based on measurement conditions including one of a plurality of dimming plates (16a to 16e) to be disposed in each wavelength range and a scanning speed of a wavelength to be set in each wavelength range, when wavelength scanning measurement in which the entire measurement wavelength range including all of the plurality of wavelength ranges is scanned at once is performed, a spectrophotometer (100) changes one of the plurality of dimming plates (16a to 16e) and the scanning speed according to the measurement conditions for each wavelength range.

A reference imaging system including a planar reference piece. The reference imaging system further includes a three-axis gantry for positioning the planar reference piece at a plurality of points in a 3D coordinate system. Additionally, the reference imaging system includes a yaw actuator for adjusting the yaw angle of the object. Furthermore, the reference imaging system includes a pitch actuator for adjusting the pitch of the object. Moreover, the reference imaging system includes a computer processing unit for controlling the 3D position, pitch and yaw of the planar reference piece.

The method presented here is used to determine the volume dispensed by any liquid handling device (automated or manual) . A reference curve is first generated by spectroscopically reading a fixed-volume set of known, variable-concentration derivatives of a single dye. During testing the liquid handling device dispenses a yet undetermined volume of known-concentration dye into a known volume of diluent which results in a new dye concentration (resultant concentration). The absorbance of the resultant concentration is then compared to the absorbance vs concentration relationship of the earlier generated reference curve to determine the volume of dye (hence volume) dispensed by the liquid handling device. This method is an alternative to the dual dye and gravimetric volume verification methodologies. It considers and corrects for the uncertainties found in a traditional single dye approach as stated in the IWA-15 ISO standard. The system and method is distributed in bundled kits including but not limited to standardized labware, a calibrated reference pipette, a proprietary Validation Reference Plate (VRP) which automates reference curve generation, a spectrophotometer calibration plate for NIST Traceability, an environmental monitor, reference reagents, test reagents, an apparatus for quality assurance and control during manufacturing, and proprietary software for data analysis and reporting.

Spectral analysis system for capturing a spectrum with an optic that forms an optical path. The spectral analysis system is configured to apply a correction function to a captured spectrum so as to obtain a modified spectrum.

Disclosed is a system for analyzing a display device, the system including: an image photographing device configured to photograph an image for inspection output from a display device to be inspected and obtain an RGB value for inspection from the photographed image; and an inspection control unit configured to convert the RGB value for inspection into a CIE XYZ value by using a previously prepared color conversion function, and determine defect of the display device to be inspected by using the converted CIE XYZ value.

A measuring method for measuring dissolved oxygen includes performing a first measurement sequence, including: emitting a first stimulation signal onto a sensor for a first period; detecting a first detection signal; determining a phase shift between the first stimulation signal and the first detection signal; and calculating a first measured value based on the determined phase shift. Performing a second measurement sequence, including a second stimulation signal onto the sensor for a second period, wherein the second stimulation signal is different than the first stimulation signal; detecting a second detection signal; determining a decay time of the second detection signal; calculating a second measured value based on the decay time. The method further includes comparing the first measured value to the second measured value and correcting the first measured value when a difference between the first measured value and the second measured value is greater than a first limit value.