The invention provides novel non-invasive in vitro methods for assessing the metabolic condition of oocytes and/or embryos with fluorescence lifetime imaging microscope, that can be used, for example, in assessment of oocytes and embryos in assisted reproductive technologies.

Described and represented is a method for determining the content of at least one plant substance of at least one part of a plant. In order for the content of plant substances, in particular secondary plant substances, of at least one part of a plant to be determined and optimized more expediently, it is provided that the at least one part of the plant is irradiated successively with light of different wavelengths and/or wavelength ranges and that, in response to the irradiation of the at least one part of the plant with light of each wavelength and/or at each wavelength range, the chlorophyll fluorescence at least substantially the same wavelength and/or at least substantially the same wavelength range is measured in each case.

The disclosed embodiments provide a system that images a tissue sample. During operation, the system receives the tissue sample, which has been stained using absorbing and fluorescently emitting stains. Next, the system illuminates the tissue sample with excitation light having a wavelength or wavelengths in a range that covers a portion of an absorption spectrum for both fluorescently emitting and absorbing stains, whereby the excitation light interacts with stained tissue located inside the tissue sample to both limit penetration depth and generate emitted dye fluorescence and tissue autofluorescence that provides a backlight, which is absorbed by features in stained tissue located on or near the surface of the tissue sample. Next, the system uses an imaging device to capture an image of emitted fluorescence that emanates from the surface of the tissue sample.

There are provided a control device of an image reading apparatus, an operation method and an operation program thereof, and an image detection system capable of quickly and easily outputting an image having an appropriate density for analysis from an image reading apparatus. An image receiving unit receives a pre-image output in pre-scanning performed before main scanning for outputting a main image for analysis in an image reading apparatus. A region information receiving unit receives information of a region in the pre-image designated by a user. A calculation unit calculates an appropriate voltage value that is a voltage value of the photomultiplier at which a density of the region becomes an appropriate density for analysis. A scanning conditions setting unit sets the appropriate voltage value as temporary scanning conditions of main scanning.

An oxygen content sensor includes a nanoparticle, a plurality of linkers, and a plurality of fluorescent molecules. The linkers are disposed on the nanoparticle. The fluorescent molecules are arranged on the linkers. The linker has at least a hydrophilic region as well as a hydrophobic region. The linker is linked to the nanoparticle through the hydrophilic region, and the fluorescent molecule is linked to the linker through the hydrophobic region.

An object carrier, a system and a method is disclosed for the back light inspection of transparent or semitransparent objects. The carrier has a carrier base layer with photo luminescent properties which carries the transparent or semitransparent object on top of the layer. The transparent or semitransparent object could be a wafer and the object carrier could be a wafer chuck. At least one light source being arranged above the object carrier such that excitation light emitted from the at least one light source is directed through the transparent or semitransparent object to the layer with photo luminescent properties. The light returned from the layer with photo luminescent properties is collected by an objective and registered by a sensor.

An interferometric system and a method of measurement of refractive index spatial distribution for use in digital holographic microscopy to observe samples in reflected as well as transmitted radiation or to observe luminescent samples comprises a first branch and a second branch with a plurality of optical elements. The first branch comprises a diffraction grating located in a plane optically conjugated with the object plane in order to create an achromatic hologram with spatial carrier frequency in the output image plane.

A gas sensor element includes: a supporting base material; a first light-emitting layer that is provided on the supporting base material and contains a first light-emitting particle which emits light at a first peak wavelength; a sensor layer that is provided on the first light-emitting layer and adsorbs gas molecules; a second light-emitting layer that is provided on the sensor layer and contains a second light-emitting particle which emits light at a second peak wavelength different from the first peak wavelength; and a protective layer that is provided on the second light-emitting layer, in which the gas sensor element has a laminated structure in which the supporting base material, the first light-emitting layer, the sensor layer, the second light-emitting layer, and the protective layer are laminated in this order, and the laminated structure includes an opening that penetrates a part or entirety of the laminated structure.

Techniques are disclosed relating to fluorescence-based flow cytometry. A flow cytometer may include a partially-reflective surface configured to reflect a first portion of fluorescent emissions from a sample to a first optical sensor and direct a second, greater portion of fluorescent emissions from the sample to a second optical sensor and a controller configured to determine a value representing the intensity of the fluorescent emissions based on a first measurement taken by the first optical sensor, a second measurement taken by the second optical sensor, or both. A flow cytometer may include a baseplate with a first side and a second, opposing side with a flow cell, a laser, and a reflective surface disposed above the first side and an optical sensor and isolating material disposed below the second side. The reflective surface receives fluorescent emissions and reflects at least a portion through the baseplate to the optical sensor. A flow cytometer may include a flow cell, a laser, a first optical sensor positioned to measure scattered laser light, a second optical sensor positioned to measure fluorescent emissions, and a controller configured to adjust the measurements taken by the second optical sensor based on a comparison of measurements taken by the first optical sensor with expected measurements based on a known beam profile of the laser beam.

An illumination module may include a laser diode array configured to emit laser radiation; a phosphor illumination unit that is configured to emit phosphor radiation following an exposure to the laser radiation; a multiple-angle illumination unit; and intermediate optics that is configured to convey the phosphor radiation to the multiple-angle illumination unit. The multiple-angle illumination unit is configured to receive the phosphor radiation and to dark field illuminate a region of a sample wafer from multiple angles during inspection of the wafer.