A method of optical analysis comprises receiving light at an optical spectrometer module from a light source, distributing the received light into two or more light beams with a light distribution component of the optical spectrometer module, concurrently exposing each of a reference and one or more test samples to one of the two or more light beams, and concurrently measuring a property of the light associated with each of the reference sample and one or more test samples with a corresponding detector.

An apparatus and method of use are provided; the apparatus having at least a degasser, a hollow core fiber HCF, an optical mechanism, a detector, and circuitry. The degasser enables gasses to permeate out of a liquid into the degasser interior. The propagator establishes a low-pressure area that helps to pull the gas from the degasser interior into the HCF interior, where the optical mechanism delivers electromagnetic radiation EMR that interacts with the gas. The detector determines EMR absorption, producing output signals which are sent to the circuitry. Circuitry controls the optical mechanism and analyzes the output signals to quantify the concentration of gas in the HCF and in the liquid.

An apparatus for measuring the absorbance of a substance in a solution, includes at least one sample cell arranged to contain the solution that is at least partially transparent to light of a predefined wavelength spectrum, at least two light passages through the at least one sample cell, each of the light passages having a known path length, an LED light source arrangement including at least two LEDs, each arranged to emit a light output with a wavelength within the predefined wavelength spectrum. A plurality of optical fibers, one for each light passage, is arranged at each LED for receiving the light output and guiding it to the light passages. A method for measuring the absorbance of a substance in a solution includes providing the LED light source arrangement with an associate fiber bundle for each LED.

Systems for measuring a concentration of a target species include a first and second tunable diode laser generating laser light at a respective first and second wavelength each corresponding to respective absorption lines of the target species. A first optical fiber is optically coupled to the first tunable diode laser, and does not support a fundamental mode at the second wavelength. A second optical fiber is coupled to the second tunable diode laser and does not support a fundamental mode at the first wavelength. A fiber bundle includes respective distal ends of the first and second optical fibers, which are stripped of their respective coatings and arranged with their claddings adjacent to each other. A pitch head is configured to project respective optical beams from the fiber bundle through a measurement zone. A catch head located across the measurement zone receives the projected beams and directs them to a sensor.

An optical measurement system is provided, which includes a light source device, a fiber module, an optical detection device and a processing circuit. The light source device is configured to generate light to illuminate a target tissue area and a reference tissue area of a human body. The fiber module is configured to direct and transmit the light to illuminate the target tissue area and the reference tissue area and receive response beams from the target tissue area and the reference tissue area. The optical detection device is configured to detect the response beams from the target tissue area to obtain the target spectrum signal and detect the response beams from the reference tissue area to obtain a reference spectrum signal. The processing circuit configured to calculate a health status parameter of the target tissue area according to the target spectrum signal and reference spectrum signal.

One aspect relates to a bioreactor and/or mixing container that includes an outer wall and a spectroscopy cell arranged in and/or on the outer wall. The spectroscopy cell includes a first optical area and a second optical area arranged opposite the first optical area. The first optical area and the second optical area can be set at at least two different distances from one another. A specimen-receiving area is located between the first optical area and the second optical area.

Hardware and control software for use in the field of digital imaging and spectroscopy. More particularly, a hardware and software system that simultaneously measures electromagnetic energy as quantities of photons in distinct wavelength regions across the ultraviolet, visible, and infrared spectrum. The system records the measurements as digital data and employs a processor (preferably a programmable processor) that executes processing steps to enhance the spatial and spectral fidelity of the recorded signals. More specifically, the electro-optical sensor hardware is engineered to maximize the light collection efficiency, especially for low light intensities, by using multiple detectors, each of which is optimized individually to maximize its sensitivity to specific wavelength regions of interest. The detector system also employs a variable amplification process that is dependent on the signal intensity so that low signals can be increased for better detection while high signals are amplified less to stay within the dynamic range of the optical sensor that is used to convert the analog signal to a digital value. Solutions to existing problems of low light detection are provided as are new capabilities for data collection and analysis in previously undetectable low signal regimes. The systems and methods are applicable to a broad array of imaging applications in diverse fields from biomedical imaging to astronomy and remote sensing.

An apparatus for measuring the absorbance of a substance in a solution includes at least one sample cell arranged to contain the solution that is at least partially transparent to light of a predefined wavelength spectrum, at least two light passages through the at least one sample cell, each of the light passages having a known path length, an LED light source arrangement including at least two LEDs, each arranged to emit a light output with a wavelength within the predefined wavelength spectrum. A plurality of optical fibers, one for each light passage, is arranged at each LED for receiving the light output and guiding it to the light passages. A method for measuring the absorbance of a substance in a solution includes providing the LED light source arrangement with an associate fiber bundle for each LED.

A Raman spectroscopy system, including an optical excitation source, an objective lens, a mirror for redirecting optical excitation to the objective lens, an optical switch, an excitation beam optical fiber operationally connected to the objective lens and to the optical switch, a plurality of Raman probes, a plurality of probe optical fibers, each respective probe optical fiber operationally connected to the optical switch and a respective Raman probe, and a spectrometer. Each respective Raman probe is operationally connected to the spectrometer.

Measuring a concentration of at least one target species is described. A first and second tunable diode laser are configured to generate laser light at a respective wavelength different from one another. A pitch head comprising a transmitting optic is optically coupled to the first and second tunable diode lasers via distal ends of the first and second optical fibers, and is oriented to project respective beams from each of the first and second distal ends through a measurement zone. A photodetector is configured to detect an optical power of light in the first and second wavelengths. A catch head located across the measurement zone from the pitch head is in optical communication with the pitch head to receive the respective beams from the first and second distal ends and direct the respective beams to the photodetector.