Match-paired monoclonal antibodies against major royal jelly protein 4 (MRJP4) are secreted by hybridoma cell lines having microbial deposit numbers of CGMCC No. 17294 and CGMCC No. 17295, which are used in an ELISA kit and a colloidal gold immunoassay strip for detecting the MRJP4. The positive and MRJP4-specific cell lines are obtained by cell fusion using an antigen of MRJP4 recombinant protein and a cross-reaction with other major royal jelly proteins. The MRJP4 recombinant protein is used as an antigen to obtain several positive cell lines by cell fusion and two MRJP4-specific fusion cell lines are obtained by a cross-reaction with other major royal jelly proteins. Primary screening of a matched antibody pair is performed according to antibody pairing for recognizing different epitopes.

The present invention provides a method for the detection of American foulbrood (AFB) in a beehive, said method comprising the steps of taking a sample from the beehive and testing the sample for a range of compounds, wherein the presence of one or more of the compounds in a relative amount of at least three times that of a non-infected hive indicates the presence of AFB in the beehive.

The present invention provides a method for studying transport of an agent across a membrane comprising the steps a) providing at least one surface with a bilayer structure tethered to the surface, said bilayer structure comprising a detection volume, b) contacting the bilayer with at least one agent to be analyzed, and c) detecting a change in refractive index in the detection volume resulting from transportation of the agent across the membrane. Further there is provided a device comprising a) at least one surface, b) at least one bilayer structure tethered to the surface, and c) at least one sensor capable of detecting a change in refractive index in a detection volume, wherein the bilayer structure encloses a first volume of the detection volume and wherein the volume not enclosed by the bilayer structure but within the detection volume is a second volume and wherein the ratio between the first volume and second volume is above about 0.001.

This disclosure relates generally to novel kits for measuring insect health, and to methods of making and using such compositions. More specifically, the invention relates to novel kits for a rapid and high-throughput measurement of lipase activity levels in insects, and the correlation of the measured lipase activity levels with insect stress.

Described herein are anti-inflammatory fractions and compounds, medicaments, methods and uses thereof. The fraction may be obtained from honey. The fraction shows a generalised anti-inflammatory response. Also described are method f producing the fraction as well as a method of testing the anti-inflammatory potential of a honey by completing the fractionation and a cell test.

Match-paired monoclonal antibodies against major royal jelly protein 4 (MRJP4) are secreted by hybridoma cell lines having microbial deposit numbers of CGMCC No. 17294 and CGMCC No. 17295, which are used in an ELISA kit and a colloidal gold immunoassay strip for detecting the MRJP4. The positive and MRJP4-sepcific cell lines are obtained by cell fusion using an antigen of MRJP4 recombinant protein and a cross-reaction with other major royal jelly proteins. The MRJP4 recombinant protein is used as an antigen to obtain several positive and cell lines by cell fusion and twoMRJP4-specific fusion cell lines are obtained by a cross-reaction with other major royal jelly proteins. Primary screening of a matched antibody pair is performed according to antibody pairing for recognizing different epitopes.

The present disclose relates to anti-inflammatory proteins/peptides, their uses, methods of preparation and methods of their detection. In particular, the invention relates to major royal jelly proteins modified by methyglyoxal and fragments thereof from a Leptospermum derived honey and royal jelly.

The present disclosure relates to anti-inflammatory proteins, their uses, methods of preparation and methods of their detection. In particular, the invention relates to major royal jelly proteins modified by methyglyoxal and fragments thereof from manuka honey.

Anti-inflammatory proteins/peptides are described, as well as their uses, methods of preparation, and methods of their detection. Specifically described are major royal jelly proteins modified by methylglyoxal and fragments thereof from a Leptospermum derived honey and royal jelly.