Method of determining therapeutic drug antibodies in a sample of bodily fluid of a subject receiving a medication containing a therapeutic drug antibodies against tumor necrosis factor alpha. The method is used in an lateral flow immunochromatographic test wherein the immunochromatographic bridging and binding in the test line comprises the use of an anti-idiotypic scFv fragment or Fab fragment fused to a carrier protein which is not involved in nor plays a role in the inherent or developed immune system. The fusion protein may contain human serum albumin, chicken ovalbumin, human haptoglobin or human alpha-1- antitrypsin. The immunological reaction is therefore not impaired, augmented or interfered by members of the complement system or by autoantibodies such as the rheumatoid factor. This is of particular importance and favorable when determining the concentration of tumor necrosis alpha blockers such as adalimumab or in-fliximab in serum or blood of patients.

The invention relates to a method for producing a device for the electrochemical detection of molecules by way of redox cycling, to a device therefor, and to the use thereof. A porous dielectric layer is present between two electrode layers, which is able to receive redox-active molecules and may be biofunctionalized. The individual layers are preferably applied by way of an ink jet printing method.

The disclosure provides methods of detecting a cannabinoid or metabolite thereof in a sample and test kits for the same. For example, the cannabinoid such as tetrahydrocannabinol (THC) bound to bovine serum albumin (BSA) may induce changes in optical properties of an optical biosensor employed for the said detection wherein the optical biosensor is immobilized with a suitable bioreceptor or through interactions with a bioreceptor-nanoparticle conjugate.

Methods and systems for detecting allergens using mass spectrometry are provided herein. In some aspects, a sample can be screened for the presence or quantity of ovalbumin, lysozyme, casein (isoform S1 and S2), lactoglobulin, high and low glutens, wheat, rye, oats, barley, mustard, sesame, and various types of nuts including macadamia, pistachio, brazil, walnuts, peanuts and hazelnuts by detecting one or more peptides specific to the allergen of interest using selected MRM transitions.

The invention relates to a method for producing a device for the electrochemical detection of molecules by way of redox cycling, to a device therefor, and to the use thereof. A porous dielectric layer is present between two electrode layers, which is able to receive redox-active molecules and may be biofunctionalized. The individual layers are preferably applied by way of an ink jet printing method.

The invention relates to methods of screening biological samples from a subject for the presence of one or more microorganisms, such as the presence in the subject of one or more pathogenic or potentially pathogenic microorganisms, and diagnostic kits and devices suitable for carrying out said methods. More particularly, the present invention relates to sensitive and specific methods for determining the presence of gram-negative bacteria or analytes therefrom in a sample from a subject using immunoassays, and devices and kits for carrying out such methods.

The present invention provides substance which can specifically interact with sugar chains. Further, the present invention provides a method for separating, concentrating, or purifying sugar chains or a sugar chain-containing substance in a sample, comprising the steps of: a) contacting a sugar chain-trapping carrier comprising a substance which can specifically interact with sugar chains with the sample in a fluid phase under conditions that the sugar chain-trapping carrier can react with the sugar chains or sugar chain-containing substance; b) isolating a composite of the sugar chain-trapping carrier and the sugar chains or sugar chain-containing substance from the fluid phase; and c) exposing the composite to the conditions that the interaction between the sugar chain-trapping carrier and the sugar chains or sugar chain-containing substance is at least partially eliminated.

The present invention is an immunosensor electrode based on a nanocomposite comprising a nanocellulose, a two-dimensional conductive nanomaterial, and a metal oxide nanoparticles to be deposited on a conductive substrate for detecting allergen. The nanocomposite with a large surface area was used as electrochemical mediator and immobilization surface for the antibody to bind with the allergen. The fabricated immunosensor exhibited high selectivity, reproducibility, and interference resistance and achieved excellent recoveries of detecting allergen in real food samples, indicating its potential applicability in food safety monitoring.