A system for analyzing a wound fluid. The system includes a transparent layer, a membrane layer, and an indicator layer that contains a colorimetric or fluorescent indicator reagent for detecting pH, a nitrite, an enzyme, a reactive oxygen species, a reactive nitrogen species, a nucleic acid, or a combination thereof. The membrane layer is impermeable to blood clots and cellular debris and is permeable to wound fluid. Also provided are methods for analyzing a wound fluid and for detecting biological fluid on biomedical instruments and waste materials using the system.

Methods and systems for conditioning a gas sample for analysis and measuring, detecting, and/or determining the concentration of at least one analyte in a gas sample. Methods include a combination and/or repetition of dehumidifying and/or humidifying the gas, and/or performing a chemical reaction an analyte, and measuring, detecting, and/or determining the concentration of an analyte or an output analyte resulting from the chemical reaction. Systems to adjust the humidity of a gas sample and/or perform a chemical reaction on an analyte, and measure, detect, and/or determine the concentration of an analyte or an output analyte resulting from the chemical reaction comprise cartridges, capsules, test strips or test strip chambers and one or more sensors. Systems may further comprise a humidity exchange material to further adjust the humidity. Gas samples include exhaled breath. Analytes include nitric oxide. Output analytes include nitrogen dioxide.

An on-diaper body fluid screening device (100), comprising a stack of the following sheets: a surface sheet (110) permeable to body fluid, a collection sheet (120) impervious to body fluid, a testing sheet (130), which is body fluid absorbent and provided with colorimetric assay reaction pads (131), and which is provided with a body fluid barrier network (132) forming body fluid channels (134) between an inlet section (135) and the colorimetric assay reaction pads (131), a protection sheet (140) impervious to body fluid, and a transparent readout sheet (150), through which the assay reaction pads (131) are visible. The protection sheet (140) comprises pad receiving openings (142). The collection sheet (120) comprises an inlet hole (125) which is arranged over the inlet section (135). A swelling component (702) is arranged in association with the inlet hole (125) and an inlet hole closing member (601).

Multiplex polymeric dye devices are provided. Aspects of the devices include a solid support, and first and second dried polymeric dye compositions distinctly positioned relative to a surface of the solid support. Aspects of the invention further include methods of making and using the devices, e.g., in analyte detection applications, as well as kits containing the devices.

A colorimetric sensor has a first material deposited on a surface, and sensing particles on a surface of the first material, wherein the sensing particles comprise sensing species dispersed into porous host structures, such that at least a portion of the sensing particles is exposed to an ambient environment, wherein the first material attaches the sensing particles to surface. A method of forming a colorimetric sensor including depositing a first material onto a substrate, providing porous sensing particles, wherein the sensing particles comprise sensing species dispersed into a porous host structure, and embedding the porous sensing particles onto a surface of the deposited first material, wherein the first material attaches the sensing particles to the substrate such that at least a portion of the sensing particles is exposed to an ambient environment.

This present disclosure provides devices, systems, and methods for performing point-of-care analysis of a target analyte in a biological fluid via a binding assay. The present disclosure includes a cartridge for collecting the target analyte contained in a fluid sample and performing an assay. The cartridge includes an assay stack having a first separation layer, a second separation layer, and a detection membrane. The cartridge also includes a plurality of first complexes comprising a capture molecule and a magnetic bead and a plurality of second complexes comprising a detection molecule and a detection label. Further, the detection membrane includes a substrate that interacts with the detection label to elicit a quantifiable response in the presence of the target analyte. The quantifiable response corresponds to an amount of detection antibody present in the detection membrane, and the amount of detection antibody present corresponds to an amount of the target analyte present.

Medical test cards for detecting analytes are provided including a substantially planar body, an analyte detection means, an opening feature, and an optical code. The analyte detection means is enclosed within the planar body and is configured to provide a colorimetric change when a portion of the analyte detection means is contacted with the analyte. The opening feature is configured to provide access to the analyte detection means and the optical code includes information identifying the analyte detection means. Uses of such medical test cards include accessing the analyte detection means enclosed within the planar body by using the opening feature and contacting the analyte detection means with the sample. The optical code is read using an imaging device to identify the analyte detection means and the analyte detection means is imaged following contact with the sample using the imaging device.

The present invention provides a chemical sensor comprising a substrate, a first colorimetric sensor array exposed and arranged in a first accommodating space of the substrate and a second colorimetric sensor array arranged in a second accommodating space of the substrate. The second accommodating space is covered with an isolating layer to isolates liquid molecules but allows gas molecules to pass through. The first colorimetric sensor array changes from a first initial color to a first indicating color according to a volatile part and a non-volatile part of an analyte, and the second colorimetric sensor array changes from a second initial color to a second indicating color according to the volatile part of the analyte, so that information of the volatile part and the non-volatile part of the analyte can be obtained simultaneously.

A moisture-responsive film can include a complexing substrate and an iodine layer applied to the complexing substrate to form the moisture-responsive film. The moisture-responsive film can include a test area formed on a testing surface of the moisture-responsive film.

A biological sample reaction vessel comprising a reagent storage portion and a push rod movable relative to the reagent storage portion is provided. The reagent storage portion comprises at least one reagent containing cavity, and the reagent containing cavity is sealed by a sealing element; and the push rod is connected to the sealing element, and the push rod is used for cooperation with an external device to separate the sealing element from the reagent storage portion. In reaction, the biological sample reaction vessel cooperates with a test cassette. By inserting the biological sample reaction vessel into the external device, the reagent in the reagent storage portion can be released rapidly.