A method of analyzing a glycated protein, including reacting a color developer represented by the following Formula (1) with a biological sample; and measuring a color reaction of the color developer,

##STR00001## wherein, in Formula (1), X is an alkali metal ion. The biological sample may be a serum sample or a plasma sample. The biological sample in a liquid state may be added to the color developer in a dry state.

The present relates, in general terms, to a device for monitoring oxidative stress in a sample, a method of making the device and a method of monitoring oxidative stress in a sample thereof.

Disclosed herein are methods of producing a test element for studying a body fluid sample. In the methods, a detection layer is covered with a polymeric spreading layer and applied to a support. The spreading layer can be produced by being sprayed onto the detection layer such that the spreading layer has a thickness of at most about 20 μm. Also disclosed are test elements produced by the methods, as well as tape cassettes incorporating such test elements.

A method of forming a colorimetric sensor includes depositing a first material onto a substrate, providing porous sensing particles, wherein the sensing particles comprise sensing species dispersed into a porous host structure, and embedding the porous sensing particles onto a surface of the deposited first material, the first material attaching the sensing particles to the substrate with at least a portion of the sensing particles is exposed to an ambient environment.

This present disclosure provides devices, systems, and methods for performing point-of-care analysis of a target analyte in a biological fluid via a binding assay. The present disclosure includes a cartridge for collecting the target analyte contained in a fluid sample and performing an assay. The cartridge includes an assay stack having a first separation layer, a second separation layer, and a detection membrane. The cartridge also includes a plurality of first complexes comprising a capture molecule and a magnetic bead and a plurality of second complexes comprising a detection molecule and a detection label. Further, the detection membrane includes a substrate that interacts with the detection label to elicit a quantifiable response in the presence of the target analyte. The quantifiable response corresponds to an amount of detection antibody present in the detection membrane, and the amount of detection antibody present corresponds to an amount of the target analyte present.

A layered dressing includes: a permeable wound contact layer for placing in contact with a wound; a breathable barrier layer; a biosensor sensing array configured to detect one or more markers in wound fluid; and a fluid collection layer disposed between the wound contact layer and breathable barrier layer and configured to deliver wound fluid by capillary action from a wound in contact with the wound contact layer to the biosensor sensing array.

Embodiments of various aspects described herein are directed to assays and devices for detecting a target molecule in a sample. In particular, there is described a lateral assay comprising a plurality of serially oriented capture zones, where each capture zone independently comprises an immobilized competitive molecule on a lateral flow matrix. The immobilized competitive molecule and the analyte competitively bind with a capture agent capable of binding the analyte.

Methods of assessing an analyte in a blood sample are provided according to aspects of the present disclosure which include: extracting the analyte from a biological sample dried on a treated support, producing an extracted sample, the treated support comprising a protein denaturant, wherein the analyte is a substrate for an enzyme present, or suspected of being present, in the biological sample, wherein the protein denaturant inhibits enzymatic activity of the enzyme on the analyte; and subjecting the extracted sample to liquid chromatography tandem mass spectrometry (LC/MS/MS), thereby assessing the analyte in the biological sample.

The present invention relates generally to an assay for detecting and differentiating single or multiple analytes, if present, in a fluid sample, including devices and methods of use of the same.

A test strip (12) includes a flow path (26) formed in a main body portion (20); a reagent portion (22b) provided in the flow path (26); and an intake portion (24) which is provided at a starting end of the flow path (26) and through which a sample is introduced into the flow path (26). The main body portion (20) is provided with a buffer space (28) communicating with a terminal end of the flow path (26), and a vent hole (30) opened at an outer surface of the main body portion (20) and communicating with the buffer space (28), and in a region where the buffer space (28) and the flow path (26) are connected, a cross-sectional area (Sb) of the buffer space (28) is larger than a cross-sectional area (S) of the flow path (26).