Some embodiments of the invention include virus-like particle (VLP) binding agents, and related polynucleotides, cells, methods of making, and compositions. Other embodiments of the invention include methods of detecting VLPs, parvovirus, erythrovirus or parvovirus B19 using a VLP binding agent and diagnostic methods for parvovirus, erythrovirus or parvovirus B19. Further embodiments include methods for administering VLP binding agents to an animal. Other embodiments include treating parvovirus, erythrovirus or parvovirus B19 infections and other diseases. Additional embodiments of the invention are also discussed.

Some embodiments of the invention include virus-like particle (VLP) binding agents, and related polynucleotides, cells, methods of making, and compositions. Other embodiments of the invention include methods of detecting VLPs, parvovirus, erythrovirus or parvovirus B19 using a VLP binding agent and diagnostic methods for parvovirus, erythrovirus or parvovirus B19. Further embodiments include methods for administering VLP binding agents to an animal. Other embodiments include treating parvovirus, erythrovirus or parvovirus B19 infections and other diseases. Additional embodiments of the invention are also discussed.

The present invention provides for water-soluble mono- and dicationic fluorescent dyes with the latter exhibiting stable fluorescence at elevated temperatures. The present invention also provides for methods for the production of the fluorescent dyes and for using these dyes in biological assays such as multiplexing qPCR and tissue staining.

The present invention provides for water-soluble mono- and dicationic fluorescent dyes with the latter exhibiting stable fluorescence at elevated temperatures. The present invention also provides for methods for the production of the fluorescent dyes and for using these dyes in biological assays such as multiplexing qPCR and tissue staining.

Described herein is a method for detecting the presence of circulating extracellular vesicles in a subject. The method comprises contacting a biological sample from the subject with an antibody mimetic that specifically binds to a cell surface marker on the vesicles, wherein the antibody mimetic is coupled to a detectable label; and detecting presence of extracellular vesicles in the sample by detecting the presence of the detectable label coupled to the antibody mimetic bound to the vesicles.

The present subject matter is directed to a luminogen exhibiting aggregation induced emission, wherein T1, T2, and T3 comprise one or more polyynes as a conjugated bridge. The present subject matter is also directed to an AIEgen comprising a hydrophilic pyridium group as a strong electron-withdrawing group; a piperazine group as an electron-donating group; and a α-Cyanostilbene; wherein the AIEgen exhibits aggregation induced emission. The present subject matter is directed to a method of synthesizing an AIEgen and is further directed to a method of labeling comprising incubating a subject having cells with a conjugate formed by conjugating an AIEgen with an antibody; and selectively labeling desired cells by turn-on imaging, wherein labeling occurs when the desired cells are selectively stained by fluorescent emission of the AIEgen upon degradation of the antibody after cellular internalization of the conjugate through endocytosis.

The present subject matter is directed to a luminogen exhibiting aggregation induced emission, wherein T1, T2, and T3 comprise one or more polyynes as a conjugated bridge. The present subject matter is also directed to an AIEgen comprising a hydrophilic pyridium group as a strong electron-withdrawing group; a piperazine group as an electron-donating group; and a α-Cyanostilbene; wherein the AIEgen exhibits aggregation induced emission. The present subject matter is directed to a method of synthesizing an AIEgen and is further directed to a method of labeling comprising incubating a subject having cells with a conjugate formed by conjugating an AIEgen with an antibody; and selectively labeling desired cells by turn-on imaging, wherein labeling occurs when the desired cells are selectively stained by fluorescent emission of the AIEgen upon degradation of the antibody after cellular internalization of the conjugate through endocytosis.

Provided is an information processing apparatus (100) including: an image acquiring unit (112) that acquires captured image information of a sample (20) dyed with a fluorescent dye reagent (10), an information acquiring unit (111) that acquires information related to the fluorescent dye reagent (10), a correcting unit (131) that corrects the luminance of the captured image information using a fluorescence fading coefficient that represents the rapidness at which the fluorescence intensity of the fluorescent dye reagent (10) drops, the fluorescence fading coefficient being included in the fluorescent dye reagent (10), and a calculating unit (132) that calculates information corresponding to fluorescent molecules in the captured image information, using the corrected luminance.

The invention relates to a fiber-optic wave guide sensor of aptamers having functions of in situ target enrichment and purification, and a method for detection of small molecules to realize the quantitative detection of small molecules targets based on that small molecules targets and the aptamers complementary short strand DNA competitively bind with aptamers tethered on the fiber surface. It synchronously realized specifically binding aptamers with targets and in situ target enrichment and purification of targets by modifying aptamers and solid micro extraction layer with silica fibers of the fiber-optic wave guide sensor, which can achieve the ultrasensitive and ultrahigh specific quick detection for all types of small molecule targets regardless of any signal amplification reaction based on enzyme. The detection limitation is very low with good generalizability.

The invention relates to a fiber-optic wave guide sensor of aptamers having functions of in situ target enrichment and purification, and a method for detection of small molecules to realize the quantitative detection of small molecules targets based on that small molecules targets and the aptamers complementary short strand DNA competitively bind with aptamers tethered on the fiber surface. It synchronously realized specifically binding aptamers with targets and in situ target enrichment and purification of targets by modifying aptamers and solid micro extraction layer with silica fibers of the fiber-optic wave guide sensor, which can achieve the ultrasensitive and ultrahigh specific quick detection for all types of small molecule targets regardless of any signal amplification reaction based on enzyme. The detection limitation is very low with good generalizability.