Methods and systems for identifying a protein within a sample are provided herein. A panel of antibodies are acquired, none of which are specific for a single protein or family of proteins. Additionally, the binding properties of the antibodies in the panel are determined. Further, the protein is iteratively exposed to a panel of antibodies. Additionally, a set of antibodies which bind the protein are determined. The identity of the protein is determined using one or more deconvolution methods based on the known binding properties of the antibodies to match the set of antibodies to a sequence of a protein.

Provided herein is a method of concentrating a tethering complex in a region of an amphiphilic layer, such as a lipid membrane. Also provided herein are methods of assembling a tethering complex; methods of concentrating an analyte in the region of a detector; amphiphilic layers; and arrays and devices for use in the disclosed methods.

The present invention provides molecular biosensors capable of signal amplification, and methods of using the molecular biosensors to detect the presence of a target molecule.

Disclosed herein are embodiments of gold nanoparticles and methods of making and using the gold nanoparticles. The disclosed gold nanoparticles have core sizes and polydispersities controlled by the methods of making the gold nanoparticles. In some embodiments, the methods of making the gold nanoparticles can concern using flow reactors and reaction conditions controlled to make gold nanoparticles having a desired core size. The gold nanoparticles disclosed herein also comprise various ligands that can be used to facilitate the use of the gold nanoparticles in a variety of applications.

Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and using the formulations, substrates, and arrays. Also disclosed are methods for identifying peptide sequences useful for diagnosis and treatment of disorders, and methods for using the peptide sequences for diagnosis and treatment of disorders, e.g., celiac disorder. In certain embodiments, substrates and arrays comprise a porous layer for synthesis and attachment of polymers or biomolecules.

Compositions and methods are described for self-assembled polymer materials having at least one of macro, meso, or micro pores.

Herein is reported a method for the determination of the amount of a bivalent antibody in a serum or plasma sample obtained from a non-human experimental animal, whereby the antibody comprises one or more mutations in the Fc-region compared to the corresponding wild-type Fc-region that has a sequence of SEQ ID NO: 01, 02, or 03, wherein the method comprises the following steps a) immobilizing a non-antibody polypeptide to which more than one copy of the antigen of the antibody is covalently conjugated on a solid surface, b) incubating the immobilized antigen with the sample to form an immobilized antigen-antibody complex, c) incubating the immobilized antigen-antibody complex with the antigen conjugated to a detectable label to form an immobilized ternary complex, and d) determining the amount of the antibody by determining the amount of the detectable label in the immobilized ternary complex.

The present invention relates to a biocompatible device which comprises on its surface an adsorbed layer of a polymer P which is a copolymer of at least one macromonomer selected from an ester E of (meth)acrylic acid and polyethylene oxide or a polyethylene glycol (meth)acrylamide, at least one monomer M selected from alkyl (meth)acrylate, aryloxyalkyl (meth)acrylate, alkyl (meth)acrylamide or aryl (meth)acrylamide, and at least one cationic monomer C selected from cationic ethylenically unsaturated N-containing monomers. It further relates to a process for making a biocompatible device which comprises on its surface an adsorbed layer of the polymer P comprising the following steps: providing a biocompatible device, and applying to the surface of the biocompatible device a solution S of the polymer Pin a solvent L. It further relates to a solution S comprising the polymer P in the solvent L, where the solvent L comprises an alcohol; and to a process for cultivating cells, comprising the following steps: providing the biocompatible device and cultivating the cells in the supernatant medium above the surface of the biocompatible device.

The present disclosure relates in some aspects to methods for preparing biological samples for in situ analysis of one or more analytes, wherein the biological sample has been previously affixed to a substrate, which is not compatible with in situ analysis, for example, due to the absence of positional markers and/or fiducial markers and/or a region suitable for in situ signal detection on the substrate.

The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.