Described herein are materials and methods of incorporating CLIP peptide into the peptide binding groove of HLA Class II antigens and using such HLA Class II antigens for the detection of alloantibodies.

Described herein are materials and methods of incorporating CLIP peptide into the peptide binding groove of HLA Class II antigens and using such HLA Class II antigens for the detection of alloantibodies.

Provide are a diagnostically useful carrier coated with a recombinant polypeptide comprising SEQ ID NO: 1, an isolated autoantibody binding specifically to a polypeptide having SEQ ID NO: 1, a kit comprising the carrier, a method for the diagnosis of a membranous nephropathy (MN) that includes detecting the presence or absence of an autoantibody binding specifically to a polypeptide having SEQ ID NO: 1 in a liquid sample comprising antibodies from a subject, a use of an autoantibody binding specifically to a polypeptide having SEQ ID NO: 1 or a polypeptide comprising SEQ ID NO: 1, and a aqueous solution comprising an autoantibody binding specifically to a polypeptide having SEQ ID NO: 1.

Disclosed is a method for preparing nanoscale electrodes comprised of electrochemically grown noble metal nanowires, and use of the same for the detection of extremely small concentrations of molecules. Such nanoscale electrodes provide target molecule release information from submicron areas on the cell surface, significantly increasing the spatial resolution of the target molecule mapping of a cell surface to enable localization of target molecules on the cell surface, which can be critical for the detection of certain cells with different properties in a given group of cells, such as circulating tumor cells.

A method for screening secretion-producing cells is provided, in which a cell that produces a secretion of interest is subjected to screening from a plurality of cells. The method includes capturing the cell and at least one detection particle that is allowed to capture the secretion of interest in each of a plurality of wells having a bottom section with a through-hole sized such that the cell does not pass through, causing the cell captured in each of the plurality of wells to produce a secretion, detecting the secretion of interest captured by the at least one detection particle, and identifying, based on a result of the detection as an index, a well in which a cell producing the secretion of interest is captured from the plurality of wells. Each of the wells has a size allowing the cell to be captured in one cell unit in a state in which one or more detection particles are captured.

A method for screening secretion-producing cells is provided, in which a cell that produces a secretion of interest is subjected to screening from a plurality of cells. The method includes capturing the cell and at least one detection particle that is allowed to capture the secretion of interest in each of a plurality of wells having a bottom section with a through-hole sized such that the cell does not pass through, causing the cell captured in each of the plurality of wells to produce a secretion, detecting the secretion of interest captured by the at least one detection particle, and identifying, based on a result of the detection as an index, a well in which a cell producing the secretion of interest is captured from the plurality of wells. Each of the wells has a size allowing the cell to be captured in one cell unit in a state in which one or more detection particles are captured.

Disclosed herein include methods, systems, compositions and kits for determining cell-cell interaction, for example, using nucleic acid sequencing. The method can match a cell barcode sequence associated with a cell of a plurality of cells with a cell barcode sequence associated with a partition of a plurality of partitions to identify the partition the cell has originated from in the plurality of partitions. In some embodiments, a pair of interacting cells within a partition are attached with a common cell barcode. The nucleic acid sequences of the pair of interacting cells having the common cell barcode can be tracked to the partition the pair of interacting cells has originated from, where phenotypic observables of the interacting cells can be obtained, for example, using optical imaging, thereby linking cell nucleic acid sequences such as expression profiles to cell functionality and the nature of the cell-cell interaction.

Disclosed herein include methods, systems, compositions and kits for determining cell-cell interaction, for example, using nucleic acid sequencing. The method can match a cell barcode sequence associated with a cell of a plurality of cells with a cell barcode sequence associated with a partition of a plurality of partitions to identify the partition the cell has originated from in the plurality of partitions. In some embodiments, a pair of interacting cells within a partition are attached with a common cell barcode. The nucleic acid sequences of the pair of interacting cells having the common cell barcode can be tracked to the partition the pair of interacting cells has originated from, where phenotypic observables of the interacting cells can be obtained, for example, using optical imaging, thereby linking cell nucleic acid sequences such as expression profiles to cell functionality and the nature of the cell-cell interaction.

The present invention relates to a method for screening an antibody or antigen-binding fragment thereof by using cells bearing magnetic beads and, more particularly, to a method for screening an antibody binding specifically to an antigen protein or an antigen-binding fragment thereof, in which cells having biotinylated phospholipids in the cell membranes thereof and a streptavidin-magnetic bead complex fused to the surfaces thereof, and a magnetic-based system are utilized.

The present invention relates to a method for screening an antibody or antigen-binding fragment thereof by using cells bearing magnetic beads and, more particularly, to a method for screening an antibody binding specifically to an antigen protein or an antigen-binding fragment thereof, in which cells having biotinylated phospholipids in the cell membranes thereof and a streptavidin-magnetic bead complex fused to the surfaces thereof, and a magnetic-based system are utilized.