Disclosed are methods and compositions which may be used in human cytochrome P450 (CYP450) enzyme phenotyping. The methods and compositions typically utilize substrate for CYP3A5 comprising eplerenone which may be administered orally to a subject. Subsequently, metabolites of eplereone may be detected in the subject's saliva as well as any non- metabolized eplerenone to calculate a metabolic ratio for CYP3A5 enzyme in order to generate a phenytopic CYP3A5 enzyme profile for the subject.

The invention provides immunochromatographic test strips for detecting digoxin and uses thereof. The immunochromatographic test strips include a bottom plate, a sample pad, a glass fiber mat, a nitrocellulose membrane and a water absorbing paper that are sequentially overlapped on the bottom plate. The glass fiber mat is sprayed with a digoxin-specific antibody-fluorescent microsphere complex. The nitrocellulose membrane is sequentially provided with a detection line coated with digoxin protein conjugate and a quality control line coated with a secondary antibody. The digoxin-specific antibody-fluorescent microsphere complex is obtained by conjugation between a digoxin monoclonal antibody or a digoxin polyclonal antibody and a fluorescent microsphere. The digoxin protein conjugate includes a digoxin-conjugated rabbit albumin or a digoxin-conjugated rabbit ovalbumin. The test strips provided by the invention are low cost, provide simple and rapid use, and have excellent stability and high sensitivity.

Disclosed are methods and compositions which may be used in human cytochrome P450 (CYP450) enzyme phenotyping. The methods and compositions typically utilize substrate for CYP3A5 comprising eplerenone which may be administered orally to a subject. Subsequently, metabolites of eplereone may be detected in the subject's saliva as well as any non- metabolized eplerenone to calculate a metabolic ratio for CYP3A5 enzyme in order to generate a phenytopic CYP3A5 enzyme profile for the subject.

The method of this invention relates a method of diagnosing a patient as having post-traumatic stress disorder (PTSD) and/or treating the patient therapeutically for PTSD. In one embodiment, the treatment involves administering a therapeutically effective amount of bufodienolide antagonist to the patient.

The present invention is directed to a target molecule modified to facilitate detection in a nanopore deice. The present invention further relates to a method of detecting such a modified target molecule using a nanopore device. It also disclose a method of using such a modified target molecule for tracking and verification of pharmaceutical, chemical or biological products and for measuring various conditions of a sample comprising the modified target molecule.

This disclosure provides compositions, including antibodies or fragments or derivatives thereof, and related devices and methods effective for detecting and quantifying olmesartan in a sample. The compositions, devices, and methods can be applied to improve the effectiveness of hypertension therapy by monitoring a subject's compliance by determining one or more pharmacokinetic parameters of the subject with a point-of-care device after antihypertensive drug administration. In one embodiment, the antihypertensive drug is olmesartan and the pharmacokinetic parameter is AUC.

This disclosure provides compositions, including antibodies or fragments or derivatives thereof, and related devices and methods effective for detecting and quantifying olmesartan in a sample. The compositions, devices, and methods can be applied to improve the effectiveness of hypertension therapy by monitoring a subject's compliance by determining one or more pharmacokinetic parameters of the subject with a point-of-care device after antihypertensive drug administration. In one embodiment, the antihypertensive drug is olmesartan and the pharmacokinetic parameter is AUC.