A microscope system is provided with a microscope that acquires images at least at a first magnification and a second magnification higher than the first magnification, and a processor. The processor is configured to specify a type of a container in which a specimen is placed, and when starting observation of the specimen placed in the container at the second magnification, the processor is configured to specify an observation start position by performing object detection according to the type of container on a first image that includes the container acquired by the microscope at the first magnification, and control a relative position of the microscope with respect to the specimen such that the observation start position is contained in a field of view at the second magnification of the microscope.

A computational microscope and a method for its operation are disclosed. In some embodiments, the microscope maps points on a sample to point in an intensity pattern on a one-to-many basis. The microscope utilizes illumination angle coding, polarization coding, amplitude coding, and phase coding to capture more information than prior art computational microscopes. Although the resulting intensity patterns are not human-interpretable images of the sample, they contain more information about the sample, by virtue of the aforementioned coding techniques, than is captured by prior-art microscopes. Machine-learning algorithms, such as neural networks, are used to analyze the intensity patterns and extract useful information, such as cellular events or cell behavior.

A computational microscope and a method for its operation are disclosed. In some embodiments, the microscope maps points on a sample to point in an intensity pattern on a one-to-many basis. The microscope utilizes illumination angle coding, polarization coding, amplitude coding, and phase coding to capture more information than prior art computational microscopes. Although the resulting intensity patterns are not human-interpretable images of the sample, they contain more information about the sample, by virtue of the aforementioned coding techniques, than is captured by prior-art microscopes. Machine-learning algorithms, such as neural networks, are used to analyze the intensity patterns and extract useful information, such as cellular events or cell behavior.

Microscopic analysis of a sample includes a system using dark-field illumination. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. A visible light source generates a light illuminating the sample on a substrate and creating a scattered field and a reflected field along a collection path of the system. A pupil mask is positioned along the collection path to block the reflected field while allowing the scattered field to pass therethrough. A camera is positioned at an end of the collection path to collect the scattered field and generate a dark-field image of the sample.

The present subject matter described an optical microscopy device (2) for a portable imaging system, such as a smartphone. The optical microscopy device (2) comprises an optical lens assembly with ten to sixteen lens elements. The optical lens assembly has an optical magnification in a range of about 1X to about 3X, an airy radius in a range of about 3.2 micron to about 15 micron, a depth of field in a range of about 28 micron to about 133 micron, a numerical aperture in a range of about 0.025 to about 0.176, a half field of view in a range of about 10 degrees to about 39 degrees, and a length in a range of about 6.8 millimeter (mm) to about 18 mm.

The present subject matter described an optical microscopy device (3) for a portable imaging system, such as a smartphone. The optical microscopy device (3) comprises an optical lens assembly with eight to fifteen lens elements. The optical lens assembly has an optical magnification in a range of about 1× to about 7.8×, an airy radius in a range of about 3 micron to about 23.25 micron, a depth of field in a range of about 20 micron to about 338 micron, a numerical aperture in a range of about 0.015 to about 0.115, a half field of view in a range of about 12 degrees to about 30 degrees, and a length in a range of about 6.5 millimeter (mm) to about 57 mm.

The present subject matter described an optical microscopy device (3) for a portable imaging system, such as a smartphone. The optical microscopy device (3) comprises an optical lens assembly with eight to fifteen lens elements. The optical lens assembly has an optical magnification in a range of about 1× to about 7.8×, an airy radius in a range of about 3 micron to about 23.25 micron, a depth of field in a range of about 20 micron to about 338 micron, a numerical aperture in a range of about 0.015 to about 0.115, a half field of view in a range of about 12 degrees to about 30 degrees, and a length in a range of about 6.5 millimeter (mm) to about 57 mm.

An immersion objective includes a correction group or correcting a spherical aberration. The displacement of the correction group along the optical axis leads to a substantially negligible defocus aberration.

An immersion objective includes a correction group or correcting a spherical aberration. The displacement of the correction group along the optical axis leads to a substantially negligible defocus aberration.

The present disclosure provides a continuous zoom stereoscopic microscope with an adjustable stereoscopic angle, consisting of a microscope stand, a first eyepiece module, a second eyepiece module, a first objective module, a second objective module, a first Risley prism, a second Risley prism, a first image-rotating prism, a second image-rotating prism, a drive module, a control module and an illumination module. The drive module provides preset drive values for individual liquid lenses in the liquid lens sets according to different magnifications to change the focal lengths of the liquid lenses, thereby changing the effective focal lengths of the objective module and that of the eyepiece module, and finally achieving continuous and fast zooming of the stereoscopic microscope to be adapted to different working scenarios. The control module controls the relative angle between the two wedge-angle prisms in the Risley prism, achieving the continuous adjusting of the stereoscopic angle of the stereoscopic microscope, and then acquiring stereo images with different stereo senses.