Methods and systems for generating non-diffracting light sheets for multicolor fluorescence microscopy are disclosed. A method for generating a non-diffracting light patterned Bessel sheet comprises transmitting an input light beam through a Fourier transform lens the input light beam has a spatial intensity pattern at a first plane, and a Fourier plane is formed after the Fourier transform lens to obtain a first light beam; transmitting the first light beam through an annulus mask to obtain a second light beam; and transmitting the second light beam through an excitation objective lens to form a non-diffracting patterned light sheet. A method for generating a non-diffracting light line Bessel sheet comprises transmitting an input light beam at a first lane through an annulus mask to obtain a first light beam; and transmitting the first light beam through an excitation objective lens to form a non-diffracting Bessel light sheet.

The present subject matter described an optical microscopy device (3) for a portable imaging system, such as a smartphone. The optical microscopy device (3) comprises an optical lens assembly with eight to fifteen lens elements. The optical lens assembly has an optical magnification in a range of about 1× to about 7.8×, an airy radius in a range of about 3 micron to about 23.25 micron, a depth of field in a range of about 20 micron to about 338 micron, a numerical aperture in a range of about 0.015 to about 0.115, a half field of view in a range of about 12 degrees to about 30 degrees, and a length in a range of about 6.5 millimeter (mm) to about 57 mm.

A light source unit includes: a housing; a semiconductor laser that is disposed in the housing and that radiates excitation light; a first condenser optical system that condenses the excitation light; a dichroic mirror that selectively reflects the excitation light; a second condenser optical system that condenses the excitation light; a wavelength conversion member that performs wavelength conversion of the excitation light and emits wavelength-converted light; an emission section that outputs the wavelength-converted light transmitted through the second condenser optical system and the dichroic mirror; and a light blocking section that is disposed between an inner surface of the housing, the inner surface being in a traveling direction of the excitation light toward a reflection surface of the dichroic mirror, and a back surface, the back surface being an opposite side of the reflection surface, or is disposed on the inner surface of the housing.

Examples relate to a microscope system comprising a Light-Emitting Diode (LED)-based illumination system and at least one image sensor assembly, and to a corresponding system, method and computer program. The LED-based illumination system is configured to emit radiation power having at least one peak at a wavelength that is tuned to an excitation wavelength of at least one fluorescent material and/or to emit radiation power across a white light spectrum, with the light emitted across the white light spectrum being filtered such that light having a wavelength spectrum that coincides with at least one fluorescence emission wavelength spectrum of the at least one fluorescent material is attenuated or blocked. The at least one image sensor assembly is configured to generate image data, with the image data (at least) representing light reflected by a sample that is illuminated by the LED-based illumination system. The microscope system comprises one or more processors, configured to process the image data to generate processed image data.

The technology disclosed present systems and methods to produce an enhanced resolution image from images of a target using structured illumination microscopy (SIM). The method includes transforming at least three images of the target captured by a sensor in a spatial domain into a Fourier domain to produce at least three frequency domain matrices that each include first blocks of complex coefficients and redundant second blocks of complex coefficients that are conjugates to the first blocks. The method includes reducing computing resources required to produce the enhanced resolution image by using first blocks of complex coefficients to produce at least three phase-separated half-matrices in the Fourier domain. The method includes performing one or more intermediate transformation on the phase-separated half-matrices to produce realigned shifted half-matrices. The method includes calculating complex coefficients of second blocks in the Fourier domain to produce full matrices from half-matrices.

According to the present disclosure, an optical signal emitted from a single molecule is received to measure a central location of the single molecule while changing a phase of a structured illumination having a periodic pattern to measure a phase of a pattern in which a fluorescence intensity is periodically changed in accordance with a distance between the pattern and the single molecule while displacing the periodic pattern by a specific interval to measure the central location of the single molecule, thereby improving an accuracy of the central location of the single molecule with low photons and as a result, the resolution of the image may be enhanced.

A system that includes an interference device including a reference arm on which a reflective surface is arranged, where the interference device produces, at each point of an imaging field when the sample is placed on a target arm of the interference device, interference between a reference wave and a target wave obtained by backscattering of incident light waves by means of a voxel of a slice of the sample at a given depth; an acquisition device suitable for acquiring, at a fixed path length difference between the target arm and the reference arm, a temporal series of N two-dimensional interferometric signals resulting from the interference produced at each point of the imaging field; and a processing unit that calculates an image representing temporal variations in intensity between said N two-dimensional interferometric signals.

A microscope device includes an opening (31) that includes a first slit and a second slit through which a plurality of pieces of light from an observation target resulting from a plurality of pieces of irradiation light emitted to the observation target and having different wavelengths pass, a dispersion element that wavelength-disperses the plurality of pieces of light passing through the opening (31), and an imaging element (32) that receives the plurality of pieces of light wavelength-dispersed by the dispersion element. The imaging element (32) performs light reception so that, as for the plurality of pieces of light wavelength-dispersed, zeroth-order light of light passing through the second slit and first-order light of light passing through the first slit do not overlap with each other.

Various embodiments include reflective-mode Fourier ptychographic microscope (RFPM) apparatuses and methods for using the RFPM. In one example, the RFPM includes a multiple-component light source configured to direct radiation to a surface. The multiple-component light source has a number of individual-light sources, each of which is configured to be activated individually. The RFPM further includes collection optics to receive radiation reflected and scattered or otherwise redirected from the surface, and a sensor element to convert received light-energy from the collection optics into an electrical-signal output. Other apparatuses, designs, and methods are disclosed.

A variable focal length lens apparatus is provided with a variable focal length lens in which a focusing position periodically changes in response to a drive signal that is input; a light source that emits detection light at an object via the variable focal length lens; an photodetector that receives the detection light that is reflected by the object, and outputs a light detection signal; a signal processor that, based on the light detection signal that is input, outputs a light emission signal that is synchronized to a focusing time point where the detection light is focused on a surface of the object; an illuminator that provides pulse illumination to the object with illuminating light, based on the light emission signal that is input; and an image capturer that captures an image of the object through the variable focal length lens.