A high content imaging system and a method of operating the high content imaging system are disclosed. A microscope has a first objective lens and a second objective lens, and an objective lens database has first and second transformation values associated with the first and the second objective lenses, respectively. A microscope controller operates the microscope with the first objective lens to develop first values of acquisition parameters. A configuration module automatically determines second values of the acquisition parameters using the first values of the acquisition parameters, first transformation values associated with the first objective lens, and second transformation values associated with the second objective lens. The microscope controller operates the microscope using the second objective lens and the second values of the acquisition parameters.

A system for selectively adjusting an objective lens correction collar includes a gear ring sized and shaped to fit about a correction collar of an objective lens. The system also includes an adjustment mechanism that has a motor operably connected to a complementary gear configured to selectively engage and cause movement of the gear ring, thereby adjusting the correction collar.

An adapter which controls rotation of a microscope on which a slide is placed and an imaging unit and which easily performs correction of a rotation shift is provided. The adapter includes a first connection member connected to a microscope, a second connection member connected to an imaging unit, a rotation member arranged between the first and second connection members and configured to rotate the second connection member relative to the first connection member using optical axes of the microscope and the imaging unit at a center, a control member configured to be fixed on one of the first and second connection members and control the rotation of the connection member, and a driving member configured to be engaged with the first connection member or the second connection member and change a position of the second connection member relative to the first connection member around the optical axes.

An observation device includes: a macro observation system; and a micro observation system. The macro observation system and the micro observation system are arranged so as to satisfy a first condition. The first condition is that a distance from a macro optical axis to a micro optical axis is equal to or less than a square root of a sum of squares of a first distance and a second distance. The first distance is a distance between the macro optical axis and a central axis of an outer diameter of the nosepiece. The second distance is a distance in a first direction between the central axis of the outer diameter and a side surface of the nosepiece. The first direction is a direction orthogonal to the macro optical axis and orthogonal to a line segment connecting the macro optical axis and the central axis of the outer diameter.

A method for viewing a microscopy specimen is described. The method includes receiving a request to change a field of view of an optical microscope system that images the microscopy specimen. In response to the request, a current field of view is automatically changed to a new field of view. Parameters of the optical microscope system are automatically adjusted to align an illumination plane of a light sheet of the optical microscope system and a detection plane of the optical microscope system. The adjustment of parameters to align the illumination plane with the detection plane is based at least on precalibrated parameters that correspond to the new field of view, the illumination path objective, and the detection path objective.

An optical system is disposed on an opposite side to an object side with respect to an objective lens, and includes: a rotational movement member that is rotationally movably supported; and a plurality of light transmissive regions that are formed in the rotational movement member, are configured to be able to be disposed at a position intersecting an optical axis of the objective lens, and have different light transmission characteristics, and a rotational movement axis of the rotational movement member is configured to be non-parallel with the optical axis of the objective lens.

A microscope system includes an objective lens, configured to gather a first light of a target sample to enter a first optical path, wherein the first light converges, at a beamsplitter, with a second light generated by an image projection module after entering the first optical path through a lens assembly; a beamsplitter assembly, configured to respectively separate and cast light in different optical paths; a camera assembly, configured to photograph the target sample in a microscope field of view, to photograph a clearly focused image through a first optical path by using the camera assembly; an auxiliary focusing device, configured to determine a focal length matching the camera assembly; and a focusing device, configured to adjust a focal length of image light entering the camera assembly according to a defocus amount of a target sample image determined by the auxiliary focusing device.

Changing device for optical components in a microscope, comprising an optical component (12) having a flat surface (13), a carrier (1) for inserting and/or holding the optical component (12), and a receptacle (26) for holding the carrier (1) in an optical path of the microscope, characterized in that the carrier (1) has bearing surfaces (8) for the flat surface (13) of the optical component (1) and positioning surfaces (10) located in the same plane, which are not covered by the optical component (12), when inserting the latter, and the receptacle (26) has bearing surfaces (28) for contact with the positioning surfaces (10), and first attachment means (11) for attaching the carrier (1) positioned on the receptacle (26) in order for the positioning surfaces (10) to act upon the bearing surfaces (28).

An apparatus for mounting an objective to a microscope structural member, and a method for operating a microscope. The apparatus for mounting an objective to a microscope structural member includes a receptacle, which is mounted or mountable to the microscope structural member, a slide-in part, which is mounted or mountable to the objective and is insertable into the receptacle where it can be brought into a locked position in which there is play between the slide-in part and the receptacle, and a tensioning unit, which, in the locked position, braces the slide-in part and the receptacle against each other in order to eliminate the play. The apparatus furthermore includes a first collision detection device, which has at least one first displacement sensor for detecting a displacement of the slide-in part and/or of the objective, in each case relative to the receptacle.

The present invention provides a technology whereby relative positioning in the horizontal direction between a specimen observation area in a specimen container and an imaging field of view can be reliably performed, even prior to adjusting the focal position in the vertical direction using an auto-focus system. This specimen observation apparatus: obtains a luminance value for an image at a plurality of locations in the specimen container, prior to performing auto-focus; and uses the number of high-luminance regions and the width of those regions and identifies a central position, in the horizontal direction, in the specimen container or uses the number of low-luminance regions and the width of those regions and identifies the central position, in the horizontal direction, in the specimen container.