A holographic storage optical system includes a storage medium, a recording unit, an imaging unit and a servo unit. The recording unit comprises a movable Fourier lens, by which the positions and irradiation angles of a signal light spot and a reference light spot are adjusted. The servo unit comprises a calibration lens for adjusting the positions of a servo light spot in the horizontal and vertical directions so that the servo light spot is located at an optimal position relative to signal light beam and reference light beam. The beam calibrating method comprises (1) before recording a data hologram, burning a calibration hologram at a calibration holographic positioning mark on an optical track of a storage medium; (2) before reproducing the data hologram, using the calibration hologram to optimize the signal-to-noise ratio of the hologram reproduced by adjusting the calibration lens and the Fourier lens.

A display system comprising a first plurality of pixels, a second plurality of pixels, a first Fourier transform lens and a second Fourier transform lens. The first plurality of pixels is arranged ranged to display first holographic data corresponding to a first holographic reconstruction and receive light of a first wavelength. The a second plurality of pixels is arranged to display second holographic data corresponding to a second holographic reconstruction and receive light of a second wavelength. The first Fourier transform lens is arranged to receive spatially modulated light having a first wavelength from the first plurality of pixels and perform an optical Fourier transform of the received light to form the first holographic reconstruction at a replay plane, wherein the first holographic reconstruction is formed of light at the first wavelength. The second Fourier transform lens is arranged to receive spatially modulated light having a second wavelength from the second plurality of pixels and perform an optical Fourier transform of the received light to form the second holographic reconstruction at the replay plane, wherein the second holographic reconstruction is formed of light at the second wavelength. The optical path length from the first Fourier transform lens to the replay plane is not equal to the optical path length from the second Fourier transform lens to the replay plane.

The present invention provides a surface shape measuring device and a surface shape measuring method which do not require a physical reference plane and can improve measurement accuracy without using a mechanical adjustment mechanism. The illumination light condensing point P.sub.Q and the reference light condensing point P.sub.L are arranged as mirror images of each other with respect to the virtual plane VP, and each data of the object light O, being a reflected light of the spherical wave illumination light Q, and the inline spherical wave reference light L is recorded on each hologram. On the virtual plane VP, the reconstructed object light hologram h.sup.V for measurement is generated, and the spherical wave optical hologram s.sup.V representing a spherical wave light emitted from the reference light condensing point P.sub.L is analytically generated. The height distribution of the surface to be measured of the object 4 is obtained from the phase distribution obtained by dividing the reconstructed object light hologram h.sup.V by the spherical wave light hologram s.sup.V. High-accuracy surface shape measurement without requiring a reference plane such as a glass substrate is realized by comparing the phase data of the reflected light acquired from the surface to be measured and the phase distribution on the plane cut surface of the spherical wave obtained analytically.

The illumination light condensing point P.sub.Q and the reference light condensing point P.sub.L are arranged as mirror images of each other with respect to the virtual plane VP, and each data of the object light O, being a reflected light of the spherical wave illumination light Q, and the inline spherical wave reference light L is recorded on each hologram. On the virtual plane VP, the reconstructed object light hologram h.sup.V for measurement is generated, and the spherical wave optical hologram s.sup.V representing a spherical wave light emitted from the reference light condensing point P.sub.L is analytically generated. The height distribution of the surface to be measured of the object 4 is obtained from the phase distribution obtained by dividing the reconstructed object light hologram h.sup.V by the spherical wave light hologram s.sup.V.

A holographic imaging device and method realizes both a transmission type and a reflection type, and also realizes a long working distance wide field of view or ultra-high resolution. Object light emitted from an object, sequentially illuminated with parallel illumination light whose incident direction is changed, is recorded on a plurality of object light holograms for each incident direction using off-axis spherical wave reference light. The reference light is recorded on a reference light hologram using in-line spherical wave reference light being in-line with the object light. An object light wave hologram and its spatial frequency spectrum at the object position are generated for each incident direction using each hologram. A synthetic spectrum which occupies a wider frequency space is generated by matching each spectrum in the overlapping area, and a synthetic object light wave hologram with increased numerical aperture is obtained thereby.

When the optical system is illuminated with an illumination light flux emitted from one extant input image point, an interference image generated by superimposing an extant output light flux output from the optical system and a reference light flux coherent with the extant output light flux is imaged to acquire interference image data, and thus to acquire measured phase distribution, and this acquisition operation is applied to each extant input image point. Thus, each measured phase distribution is expanded by expanding functions μn(u, v) having coordinates (u, v) on a phase defining plane as a variable to be represented as a sum with coefficients Σn{Ajn.Math.μn(u, v)}. When the optical system is illuminated with a virtual illumination light flux, a phase Ψ(u, v) of a virtual output light flux is determined by performing interpolation calculation based on coordinates of a virtual light emitting point.

The invention relates to a system (1) for observing objects, including: a light source (3), a holder (12) able to receive a translucent or opaque substrate, a detector (7) able to collect the backscattered light from the interaction between the light emitted by light source (3) and the objects, a polarization splitter (9), and a quarter-wave plate (10), the splitter (9) and the quarter-wave plate (10) being arranged so that the splitter (9) directs the light emitted by the light source (3) toward the solid substrate and directs the backscattered light from the interaction between the light emitted by the light source (3) and the objects toward the detector (7).

Among other things, a method comprises imaging a sample displaced between a sensor surface and a surface of a microscopy sample chamber to produce an image of at least a part of the sample. The image is produced using lensless optical microscopy, and the sample contains at least blood from a subject. The method also comprises automatically differentiating cells of different types in the image, generating a count of one or more cell types based on the automatic differentiation, and deriving a radiation dose the subject has absorbed based on the count.

A method for regulating the relative position of an analyte of a sample (16) in relation to a light beam (F) includes the illumination of the analyte of the sample (16) with the light beam (F), capturing by an imaging device (38) a transmission image of the beams scattered by the analyte of the sample (16) in order to establish a diffraction pattern, and modifying the relative position of the analyte of the sample (16) in relation to the light beam (F) according to at least one property of the diffraction pattern.

An apparatus for manufacturing a hologram includes a holographic optical element on which a first interference pattern of a first signal beam and a first reference beam is recorded and a second interference pattern of a second signal beam modulated by a Fourier lens and a second reference beam is recorded. Also, an apparatus for reconstructing a hologram by using the holographic optical element is provided.