A method for observing a sample (10), the sample lying in a plane of the sample defining radial coordinates, the method comprising the following steps: a) illuminating the sample using a light source (11), able to emit an incident light wave (12) that propagates toward the sample along a propagation axis (Z); b) acquiring, using an image sensor (16), an image (I.sub.0) of the sample (10), said image being formed in a detection plane (P.sub.0), the sample being placed between the light source (11) and the image sensor (16), such that the incident light wave sees an optical path difference, parallel to the propagation axis (Z), by passing through the sample; c) processing the image acquired by the image sensor;
wherein the processing of the acquired image comprises taking into account vectors of parameters, respectively defined at a plurality of radial coordinates, in the plane of the sample, each vector of parameters being associated with one radial coordinate, and comprising a term representative of an optical parameter of the sample, at least one optical parameter being an optical path difference induced by the sample at the radial coordinate, the vectors of parameters describing the sample.

An apparatus and method to produce a hologram of an object includes an electromagnetic radiation assembly configured to receive a received electromagnetic radiation, such as light, from the object. The electromagnetic radiation assembly is further configured to diffract the received electromagnetic radiation and transmit a diffracted electromagnetic radiation. An image capture assembly is configured to capture an image of the diffracted electromagnetic radiation and produce the hologram of the object from the captured image.

Both a hologram and fluorescence are simultaneously captured in a state in which they can be reconstructed separately. A recording device (10) includes: a laser light source (LS1) which irradiates a subject (13) with object illumination light so that object light is generated; and an image capturing device (12) which captures (i) a hologram formed by interference between reference light and object light and (ii) an image of fluorescence, and the object illumination light further excites a fluorescent material (14) contained in the subject (13).

The present disclosure provides improved systems and methods for automated cell identification/classification. More particularly, the present disclosure provides advantageous systems and methods for automated cell identification/classification using shearing interferometry with a digital holographic microscope. The present disclosure provides for a compact, low-cost, and field-portable 3D printed system for automatic cell identification/classification using a common path shearing interferometry with digital holographic microscopy. This system has demonstrated good results for sickle cell disease identification with human blood cells. The present disclosure provides that a robust, low cost cell identification/classification system based on shearing interferometry can be used for accurate cell identification. For example, by combining both the static features of the cell along with information on the cell motility, classification can be performed to determine the type of cell present in addition to the state of the cell (e.g., diseased vs. healthy).

According to an embodiment, a holographic microscope comprises a light source emitting light to an object, a beam splitter reflecting the light emitted from the light source to the object and transmitting object light reflected from the object, a holographic image sensor sensing information, including a holographic image, by receiving the object light and allowing the object light to coherently interfere with reference light, and an image processor obtaining three-dimensional (3D) information about the object based on the information sensed by the holographic image sensor. The holographic image sensor includes a lens focusing the object light to the holographic image sensor, a filter transmitting a predetermined wavelength band of light of the focused object light, a light receiving unit receiving interference light to sense a holographic image, and a reference light source directly emitting the reference light having the predetermined wavelength band to the light receiving unit.

According to an embodiment, a holographic image sensor comprises a lens focusing object light incident from outside of the holographic image sensor to the holographic image sensor, a filter transmitting a predetermined wavelength band of light of the focused object light, a light receiving unit receiving interference light to sense a holographic image, and a reference light source directly emitting reference light having the predetermined wavelength band to the light receiving unit.

A device and method for measuring a surface of an object, including at least one light source, at least one optical sensor, and an interferometry device having a measurement arm and a reference arm, the former directing light from each light source towards the surface of the object and directing light from the surface towards each optical sensor; the measurement device, in an interferometry configuration, illuminating the reference arm and the measurement arm with each light source and directing the light from the measurement arm and the reference arm towards each optical sensor to form an interference signal; the measurement device, in an imaging configuration illuminating at least the measurement arm and directing the light from the measurement arm towards the optical sensor to form an image of the surface; the measurement device including a digital processor producing, from the interference signal and the image, information on the surface.

The holographic lens system includes a geometric phase lens located on plane of an aperture, a front lens and a rear lens respectively located at the front and behind of the aperture, a polarizer located between the geometric phase lens and the front lens, and an image sensor that is located behind the rear lens and acquires an interference fringe generated by the geometric phase lens.

Disclosed herein a device acquiring holography and system including the same. The device includes: a beam splitter module splitting a light emitted from an object into a first beam and a second beam which have polarizations in different states; and an optical control module equipped with a first reflective optical element, which is disposed at one side of the beam splitter module and receives and emits the first beam to the beam splitter module, and a second reflective optical element which is placed at the other side of the beam splitter module, receives the second beam and emits the second beam to the beam splitter module so as to have differences of optical path and wavefront from the first beam. The beam splitter module, the first reflective optical element and the second reflective optical element are monolithically installed by being fixed to each other.

A method and system for performing incoherent color holographic microscopy imaging using light of various wavelengths, including modulating radiation at each wavelength to form two beams and detecting their intensity at a detector. The two beams include phase information that is retrieved from the phase shifted intensity recorded at the detector and holographic information is determined from the detected modulation of the two beams for each color. A processor is configured to receive the holographic information via a signal generated by the detector and the processor further generates a three-dimensional image of a target.