A holographic imaging device is disclosed. In one aspect, the holographic imaging device comprises an imaging unit comprising at least two light sources, wherein the imaging unit is configured to illuminate an object by emitting at least two light beams with the at least two light sources. A first and second light beams have different wave-vectors and wavelengths. The holographic imaging device further comprises a processing unit configured to obtain at least two holograms of the object by controlling the imaging unit to sequentially illuminate the object with respectively the first light beam and the second light beam, construct at least two 2D image slices based on the at least two holograms, wherein each 2D image slice is constructed at a determined depth within the object volume, and generate a three-dimensional image of the object based on a combination of the 2D image slices.

A method of generating three-dimensional (3D) shape information of an object to be measured from an image including intensity information of an object hologram generated by interference between a reference light reflected from an optical mirror and an object light affected by the object includes checking at least one frequency component included in the image and extracting real image components corresponding to a real image from the frequency component. The method also includes generating a correction light and a real image hologram based on the real image components, generating an intermediate hologram based on the correction light, and generating curvature aberration correction information from the intermediate hologram. The method further includes generating a correction hologram based on the curvature aberration correction information and generating the 3D shape information of the object from the correction hologram.

An optical inspection apparatus includes an interferometer module, which is configured to direct a beam of coherent light toward an area under inspection and to produce a first image of interference fringes of the area. The apparatus also includes a triangulation module configured to project a pattern of structured light onto the area, and at least one image sensor configured to capture the first image of interference fringes and a second image of the pattern that is reflected from the area. Beam combiner optics are configured to direct the beam of coherent light and the projected pattern to impinge on the same location on the area. A processor is configured to process the first and second images in order to generate a 3D map of the area.

The invention relates to a device, such as a digital holographic microscope, for detecting and processing a first full image of a measurement object, measured with a first offset, wherein an arrangement is provided for generating at least one further full image with at least one offset that differs from the first offset.

A method of projecting a first image and a second image using one multi-wavelength hologram. The first image is different to the second image. The multi-wavelength hologram is arranged for illumination by light of a first wavelength to project the first image. The multi-wavelength hologram is further arranged for illumination by light of a second, shorter wavelength to project the second image.

An image processing apparatus includes an acquisition unit that acquires a hologram obtained by imaging a plurality of granules contained within an imaging visual field, a generation unit that generates, from the hologram, phase difference images at positions different from each other in an optical axis direction in a case in which the hologram is captured, a specifying unit that specifies a plurality of image ranges in a direction of a plane intersecting the optical axis direction, which correspond to the plurality of granules, in an averaged image obtained by averaging at least some of the phase difference images, and an extraction unit that extracts the phase difference image at a center position of a corresponding granule in the optical axis direction for each of the plurality of image ranges.

This application relates to a method of generating three-dimensional (3D) shape information of an object to be measured from an image including intensity information of an object hologram generated by interference between a reference light reflected from an optical mirror and an object light affected by the object. In one aspect, the method includes checking at least one frequency component included in the image and extracting real image components corresponding to a real image from the frequency component. The method also includes generating a correction light and a real image hologram based on the real image components, generating an intermediate hologram based on the correction light, and generating curvature aberration correction information from the intermediate hologram. The method further includes generating a correction hologram based on the curvature aberration correction information and generating the 3D shape information of the object from the correction hologram.

A method for observing a sample, the sample lying in a sample plane defining radial positions, parameters of the sample being defined at each radial position, the method comprising: a) illuminating the sample using a light source, emitting an incident light wave that propagates toward the sample; b) acquiring, using an image sensor, an image of the sample, said image being formed in a detection plane, the sample being placed between the light source and the image sensor; c) processing the image acquired by the image sensor, so as to obtain an image of the sample, the image of the sample corresponding to a distribution of at least one parameter of the sample describing the sample in the sample plane; wherein the processing of the acquired image comprises implementing an iterative method, followed by applying a supervised machine learning algorithm, so as to obtain an initialization image intended to initialize the iterative method.

There is provided a method including generating a phase contrast image of an aggregate of a pluripotent stem cell from a hologram in which the aggregate is captured; deriving, based on the phase contrast image, an index value indicating a complexity or simplicity of a contour line, the index value being determined according to a relationship between an area of the aggregate and a length of the contour line of the aggregate; and sorting the pluripotent stem cell based on the index value.

There is provided a method including generating a phase contrast image of an aggregate of a pluripotent stem cell from a hologram in which the aggregates is captured; deriving a phase contrast amount density obtained by dividing a total phase contrast amount which is a value obtained by integrating a phase contrast amount of each of a plurality of pixels that constitute the phase contrast image, by a volume of the aggregate; and sorting the pluripotent stem cell based on the phase contrast amount density.