Quantitative methods for optimizing the permeabilization of cellular tissues for spatial transcriptomics are provided. Also provided is an instrument for quantitatively optimizing the permeabilization of cellular tissues used for spatial transcriptomics.

Diagnosis is assisted by acquiring microscopical observation image data while specifying the position, classifying the image data into histological types with the use of AI, and reconstructing the classification result in a whole lesion. There is provided a pathological diagnosis assisting method that can provide an assistance technology which performs a pathological diagnosis efficiently with satisfactory accuracy by HE staining which is usually used by pathologists. Furthermore, there are provided a pathological diagnosis assisting system, a pathological diagnosis assisting program, and a pre-trained model.

A method for measuring distorted illumination patterns and correcting image artifacts in structured illumination microscopy. The method includes the steps of generating an illumination pattern by interfering multiple beams, modulating a scanning speed or an intensity of a scanning laser, or projecting a mask onto an object; taking multiple exposures of the object with the illumination pattern shifting in phase; and applying Fourier transform to the multiple exposures to produce multiple raw images. Thereafter, the multiple raw images are used to form and then solve a linear equation set to obtain multiple portions of a Fourier space image of the object. A circular 2-D low pass filter and a Fourier Transform are then applied to the portions. A pattern distortion phase map is calculated and then corrected by making a coefficient matrix of the linear equation set varying in phase, which is solved in the spatial domain.

This disclosure relates to a model training method and apparatus and an image processing method and apparatus. The model training method includes: obtaining a first sample image and a first standard region proportion corresponding to a first object in the first sample image; obtaining a standard region segmentation result corresponding to the first sample image based on the first standard region proportion; and training a first initial segmentation model based on the first sample image and the standard region segmentation result, to obtain a first target segmentation model.

The present disclosure relates to a method and an apparatus for measuring motility of ciliated cells in a respiratory tract. The method includes the operations of: acquiring image data including a plurality of frames of respiratory tract organoids; identifying positions of ciliated cells by performing motion-contrast imaging on the image data; when a region of interest (ROI) related to the position of the ciliated cells is selected, measuring a ciliary beat frequency (CBF) related to motility of cilia included in the selected region of interest using cross-correlation between the plurality of frames; and expressing the cilia included in the region of interest in a preset display method on the basis of the range of the measured ciliary beat frequency.

A coating analyzer is configured to receive electronic image data of a physical coating and to generate information regarding the pigments of the physical coating. The coating analyzer applies a computer vision model trained on baseline image data to the electronic image data. The coating analyzer assigns color values to the pigments forming the electronic image data and generates pigment groups based on the assigned color values. The pigment groups provide color palette data regarding the pigments forming the coating.

In one embodiment, a method is provided. The method includes obtaining a sequence of images of a three-dimensional volume of a material. The method also includes determining a set of features based on the sequence of images and a first neural network. The set of features indicate microstructure features of the material. The method further includes determining a set of material properties of the three-dimensional volume of the material based on the set of features and a first transformer network.

In a method for processing microscope images, at least one microscope image is provided as input image for an image processing algorithm. An output image is created from the input image by means of the image processing algorithm. The creation of the output image comprises adding low-frequency components for representing solidity of image structures of the input image to the input image, wherein the low-frequency components at least depend on high-frequency components of these image structures and wherein high-frequency components are defined by a higher spatial frequency than low-frequency components. A corresponding computer program and microscope system are likewise described.

Methods and systems for performing diagnostic functions for a deep learning model are provided. One system includes one or more components executed by one or more computer subsystems. The one or more components include a deep learning model configured for determining information from an image generated for a specimen by an imaging tool. The one or more components also include a diagnostic component configured for determining one or more causal portions of the image that resulted in the information being determined and for performing one or more functions based on the determined one or more causal portions of the image.

A system for performing adaptive focusing of a microscopy device comprises a microscopy device configured to acquire microscopy images depicting cells and one or more processors executing instructions for performing a method that includes extracting pixels from the microscopy images. Each set of pixels corresponds to an independent cell. The method further includes using a trained classifier to assign one of a plurality of image quality labels to each set of pixels indicating the degree to which the independent cell is in focus. If the image quality labels corresponding to the sets of pixels indicate that the cells are out of focus, a focal length adjustment for adjusting focus of the microscopy device is determined using a trained machine learning model. Then, executable instructions are sent to the microscopy device to perform the focal length adjustment.