The disclosure provides genetically engineered microorganisms capable of producing antigen-binding molecules. Additionally, the disclosure provides engineered microorganisms comprising one or more disrupted genes to strategically divert carbon flux away from undesirable products towards products, and optionally co-products, of interest. Further, the disclosure enables co-production of useful chemicals from gaseous substrates.

Certain booster compositions and methods of using the booster compositions to clean fermentation equipment are disclosed. The booster compositions are useful in environments with variable, changing, unstable, or limited alkalinity sources found within fermentation equipment.

A fermentation process includes contacting a starch hydrolysate with a glucoamylase with agitation. And allowing for settling to form a multi-phase solution. A first phase of the multi-phase solution includes a saccharide component comprising about 30 wt % to about 80 wt % (e.g., 30 wt % to 70 wt %, 30 wt % to 60 wt %) based on a total carbohydrate present. The method further includes draining the first phase to isolate the first phase from a second phase to form a fermentation broth comprising a first portion of the first phase. The method further includes fermenting the fermentation broth until a concentration of glucose in the fermentation broth is 40 g/L or less. The method includes adding a second portion of the first phase to the fermentation broth to maintain a concentration of glucose in a range of from about 1 g/L to about 20 g/L in the fermentation broth.

A method for producing alcoholic beverages from alkaline cooking, using as a basis the increase in pH in the medium to cook cacahuazintle corn grains, other similar mealy corn or any other grain, which offers a different proposal due to the generation of flavors, aromas and sensory characteristics that differentiate the products obtained by the methods herein disclosed from existing alcoholic beverages. A method for producing alcohol for human consumption and/or industrial use from the recovery and treatment of nejayote obtained from alkaline cooking residues, also known as nixtamalization of corn, applied to any alkaline cooking residue in the alimentary field.

The present disclosure generally relates to a pretreatment arrangement (100) for pretreatment of lignocellulosic biomass comprising a reactor vessel (101) extending along a longitudinal center line (102) and having an upstream inlet (103) for receiving biomass and a downstream outlet (104) for discharging biomass. The pretreatment arrangement (100) further comprising a scraping device (108) configured to scrape the interior walls (111) of the reactor vessel (101) and prevent the formation and build-up of deposits.

The technology provided herein relates to novel methods for producing lactic acid (L-lactic acid, D-lactic acid and D/L-Lactic acid) from starch containing material with extreme thermophilic bacterial cells belonging to the genus Caldicellulosiruptor, mutants thereof, isolated strains, microbial cultures, and microbial compositions. The novel methods are in particular suitable for the production of lactic acid from any carbon source, not limited to but especially useful for unmodified starch and/or starch-containing material.

A process for producing a protein product from plants and yeasts comprises providing a thin stillage or a thin stillage concentrate; separating a protein concentrate from the thin stillage; diluting the protein concentrate to a dry substance content of at most 15 wt.%, tempering the diluted protein concentrate to at least 60° C., and adjusting the pH of the diluted and tempered protein concentrate to alkaline; cooling the processed protein concentrate and subsequently separating a liquid phase; adjusting the pH of the separated liquid phase to an acidic pH, and then separating a solid phase; and dispersing the solid phase in a solvent and subsequently separating a product phase, wherein the product phase comprises the protein product from plants and yeasts.

The present invention relates to a process for the production of bio-oil-based renewable streams for composing a diesel or aviation kerosene (AVK) pool by combining the technologies of hydroprocessing esters and fatty acids (HEFA) and thermochemical conversion of lignocellulosic biomass for generating bio-oil. The integration of conventional raw material from the HEFA pathway with a bio-oil fraction has the advantages of the use of a stream originating from the conversion of lignocellulosic material, which is widely available on the market, and also the production of a renewable stream containing aromatics in its composition.

Provided are a method for producing an organic substance and an apparatus for producing an organic substance that are capable of efficiently cooling a synthesis gas and converting the synthesis gas to an organic substance at a high conversion efficiency using a microbial catalyst. A method for producing an organic substance includes a step of passing a synthesis gas G1 discharged from a gasifier 2 through a heat exchanger 20 to cool the synthesis gas G1, a step of passing the synthesis gas G1 cooled with the heat exchanger 20 through a gas cooling tower 21 to cool the synthesis gas G1 with water sprayed in the gas cooling tower 21 and a step of bringing the synthesis gas G1 that has passed through at least the heat exchanger 20 and the gas cooling tower 21 into contact with a microbial catalyst to generate an organic substance.

The present invention addresses to a process for the integrated production of H.sub.2 and aviation kerosene from renewable raw materials aiming at reducing CO.sub.2 emissions and consequently bringing benefits to reduce the impact of global warming on the planet. The process involves a hydrotreatment section to obtain n-paraffins followed by a hydroisomerization section to produce isoparaffins. The water and light hydrocarbons obtained in the isoparaffin production process are used for the production of H.sub.2 by the steam reforming process. An alcohol, such as ethanol or glycerin, with less than 6 carbon atoms, is fed into the hydrotreating section to make up the light hydrocarbon stream used in the production of renewable hydrogen.