PREPARATION METHOD AND SECONDARY DISPERSION OF MONODISPERSE AMINATED MANODIAMOND COLLOID SOLUTION AND ITS APPLICATION IN CELLULAR BIOMARKING

Abstract

A preparation method and secondary dispersion of monodisperse aminated Nanodiamond colloid solution and its application in cellular biomarking are provided. Preparation method comprise: mixing purified Nanodiamond powder with ammonium chloride and sodium chloride, placing the mixture in a ball mill for dry ball milling, washing the ball-milled mixture with deionized water, and performing ultrasonic dispersion and centrifuging to obtain the monodispersed aminated Nanodiamond colloid solution. Secondary dispersion process comprising: drying aminated Nanodiamond colloid solution to obtain aminated Nanodiamond powder, re-dispersing the powder in DMSO (dimethyl sulphoxide), deionized water, ethanol, DMF (dimethylformamide) or other solvents with ultrasonic or shearing processing. The aminated Nanodiamond has high yield and good monodispersity. The preparation method is simple to operate, no special requirements on reaction equipment, no inert gas atmosphere is required in the whole reaction process and it is easy to be industrialized. The aminated Nanodiamond can be applied to cellular biomarking.

Claims

1. A method for preparing a monodisperse ammoniated Nanodiamond colloid solution, comprising steps of: mixing a purified Nanodiamond powder of appropriate particle size with ammonium chloride and sodium chloride in a reasonable mass ratio at room temperature, placing in a ball mill for dry ball milling, washing with deionized water, dispersing with ultrasonic, and centrifuging to obtain monodisperse amine Nanodiamond black colloid solution.

2. The method, as recited in claim 1, wherein the appropriate particle size of the purified Nanodiamond powder is in a range of 30 nm-100 m.

3. The method, as recited in claim 1, wherein the reasonable mass ratio of the purified Nanodiamond to deionized water is in a range of 1:20-2000, the reasonable mass ratio of ammonium chloride and sodium chloride is in a range of 1:2-100.

4. The method, as recited in claim 1, wherein the reasonable mass ratio of the purified Nanodiamond powder to the ammonium chloride is in a range of 1:0.1-100.

5. The method, as recited in claim 1, wherein adding ball milling beads in a ball milling process, the ball milling beads are non-metallic ball milling beads, including but not limited to agate, corundum, zirconia, and silica.

6. A method for secondary dispersion of monodisperse aminated Nanodiamond colloid solution prepared by the method according to claim 1, comprising steps of: (i) drying an aminated Nanodiamond black colloid solution prepared by the method according to claim 1 to obtain aminated Nanodiamond powder; (ii) dispersing the aminated Nanodiamond powder prepared in step (i) in solvent once again with an ultrasonic or shearing processing, shaking to obtain clear and transparent black colloid solution.

7. The method, as recited in claim 6, wherein a drying process in step (i) comprise any one of rotary evaporator drying, spray drying or freeze drying.

8. The method, as recited in claim 6, wherein the solvent in step (ii) comprise at least one of deionized water, dimethyl sulfoxide, ethanol, ethylene glycol, or dimethylformamide, or other composite solvents containing any of the solvents with a volume ratio over 20%.

9. Use of aminated Nanodiamonds in cellular biomarking, wherein preparing the according to claim 1.

10. The use, as recited in claim 9, wherein a concentration of aminated Nanodiamonds is in a range of 0.1-2 mg/ml.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0031] FIG. 1 shows a XRD patterns of Nanodiamond raw material (a), purified Nanodiamond (b), and aminated Nanodiamond (c) of example 3;

[0032] FIG. 2 shows a FTIR spectra of purified Nanodiamond (a), and aminated Nanodiamond (b) of example 3;

[0033] FIG. 3 shows a photo of Nanodiamond colloid solution in different amination reagent ratio conditions of example 1 to 5;

[0034] FIG. 4 shows a particle size distribution of Nanodiamond colloid solution in different amination reagent ratio conditions of example 1 to 5.

[0035] FIG. 5 shows a Zeta potential-pH graph of Nanodiamond colloid solution in different amination reagent ratio conditions of example 1 to 5;

[0036] FIG. 6 shows a photo of colloid solution of aminated Nanodiamond prepared in example 3 for secondary dispersion in different solvents;

[0037] FIG. 7 shows a particle size distribution of colloid solution of aminated Nanodiamond prepared in example 3 for secondary dispersion in different solvents;

[0038] FIG. 8 shows a TEM images of aminated Nanodiamond prepared in example 3;

[0039] FIG. 9 shows the images of different cells cultured in aminated Nanodiamond mixture for 30 minutes: FIG. 9A HepG2, FIG. 9B HeLa, FIG. 9C L02, FIG. 9D H3B, FIG. 9E NIH 3T3; a bright field picture is on the left, and a fluorescence field picture is on the right.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0040] In order to make objects, technical solutions and advantages of the present disclosure clearer, the present disclosure is further described in detail with examples. The devices and reagents used in the examples are commercially available unless otherwise noted.

[0041] The examples are only for explaining the present disclosure, not for limiting the present disclosure, since various modifications and substitutions can be made without departing from the present disclosure. As such, further modifications and equivalents of the present disclosure herein disclosed may occur to persons having ordinary skills in the art using no more than routine experimentation.

[0042] For the present disclosure to be better understood and not limited by scope, all numbers in this application that express amounts, percentages, and other values should be understood as modified by the word about in all cases. Therefore, unless otherwise specified, the numerical parameters listed in the specification and the appended claims are approximate values, which may be changed according to the desired properties that are attempted to be obtained. Each numerical parameter should at least be regarded as obtained based on the reported significant figures and through conventional rounding methods.

[0043] In order to increase the yield of aminated Nanodiamonds, the Nanodiamonds in the following examples are all purified, and the process flow are described as follows.

[0044] 10 g Nanodiamond gray powder is used to mix with 30 g50 g concentrated sulfuric acid and 10 g potassium permanganate in a reactor, 220 C. for 8 h. Nanodiamond gray powder is oxidized and purified in concentrated sulfuric acid medium by potassium permanganate in high temperature environment. After that, it is continuously cleaned with deionized water by ultrasonic to neutrality. Purified Nanodiamond powder is isolated by centrifugation and drying. A diamond content in Nanodiamond gray powder is greater than 90%; a mass fraction of concentrated sulfuric acid is 98%, both of which can be purchased in commercial.

Example 1

[0045] In this example, a preparation method of monodisperse aminated Nanodiamond colloid solution are described as follows.

[0046] (1) Ball milling beads (a large agate bead diameter of 6.8 mm and a small bead diameter of 4.2 mm, mass ratio of 1:5) were put into a ball milling tank with the volume of 100 ml, to mix with 0.5 g purified Nanodiamond and 2.5 g chlorinated Sodium, no ammonium chloride. The ball mill (model: QM-1SP2, Nanjing University Instrument Factory) with a linear speed of 512 m/min works for 2 h. Take off the ball mill tank after turning off the ball mill for 30 minutes.

[0047] (2) The ball milling beads in the ball milling tank were washed with deionized water (250300 ml, the volume of deionized water can be appropriately increased), and dispersed with ultrasonic (8000 W ultrasound generally did not exceed 1 min, 360 W ultrasound generally 15 min), and then centrifuged for 5 min with a speed of 10000 r/min, repeating 4 to 5 times to obtain clear and transparent monodisperse aminated Nanodiamond black colloid solution, which was characterized by dynamic light scattering (DLS), and the results were shown in FIG. 4.

[0048] In this example, a secondary dispersion technology of monodisperse ammoniated Nanodiamond colloid solution are described as follows.

[0049] (1) Water solvent in the clear and transparent Nanodiamond black colloid solution was removed with a rotary evaporator (model: N-1001, Shanghai Ailang Instrument Co., Ltd.), and the temperature of water bath (model: SB-2000, Shanghai Ailang Instrument Co., Ltd.) was 80 C. In this way, aminated Nanodiamond powder was obtained.

[0050] (2) The aminated Nanodiamond powder was dissolved in DMF by ultrasonic for 15 minutes to obtain clear and transparent black colloid solution of Nanodiamond with higher concentration, which was characterized by dynamic light scattering, and the result was shown in FIG. 7. The median size of the aminated Nanodiamonds in the colloid solution prepared in this example was about 50 nm, and the average particle size of the aminated Nanodiamonds in the colloid solution obtained by the secondary dispersion was about 60 nm.

Example 2

[0051] In this example, a preparation method of monodisperse aminated Nanodiamond colloid solution are described as follows.

[0052] (1) The ball milling beads (a large agate bead diameter of 6.8 mm and a small bead diameter of 4.2 mm, mass ratio of 1:5) were put into a ball milling tank with the volume of 100 ml, to mix with 0.5 g purified Nanodiamond and 2.5 g chlorinated Sodium, 2.5 g ammonium chloride. The ball mill (model: QM-1SP2, Nanjing University Instrument Factory) with a linear speed of 512 m/min works for 2 h. Take off the ball mill tank after turning off the ball mill for 30 minutes.

[0053] (2) The ball milling beads in the ball milling tank were washed with deionized water (250300 ml, the volume of deionized water can be appropriately increased), and dispersed with ultrasonic (8000 W ultrasound generally did not exceed 1 min, 360 W ultrasound generally 15 min), and then centrifuged for 5 min with a speed of 10000 r/min, repeating 4 to 5 times to obtain clear and transparent monodisperse aminated Nanodiamond black colloid solution, which was characterized by dynamic light scattering (DLS), and the results were shown in FIG. 4; analyzed by transmission electron microscope, and its morphology was shown in FIG. 8.

[0054] In this example, a secondary dispersion technology of a monodisperse ammoniated Nanodiamond colloid solution are described as follows.

[0055] (1) Water solvent in the clear and transparent Nanodiamond black colloid solution was removed with a rotary evaporator (model: N-1001, Shanghai Ailang Instrument Co., Ltd.), and the temperature of the water bath (model: SB-2000, Shanghai Ailang Instrument Co., Ltd.) was 80 C. In this way, aminated Nanodiamond powder was obtained. The yield of Nanodiamond after amination modification cab be calculated using the following Eq.

Yield=m.sub.1/m.sub.0100%

[0056] Where, m.sub.0the mass of Nanodiamond raw material, in g;

[0057] m.sub.1the mass of aminated nanodiamond after drying, in g;

[0058] In this example, the mass of the dispersed Nanodiamond after amination modification was 0.473 g, and the yield was 94.6%.

[0059] (2) The aminated Nanodiamond powder was dissolved in deionized water by ultrasonic for 15 minutes to obtain clear and transparent black colloid solution of Nanodiamond with higher concentration, which was characterized by dynamic light scattering, and the result was shown in FIG. 7. The median size of the aminated Nanodiamonds in the colloid solution prepared in this example was about 50 nm, and the average particle size of the aminated Nanodiamonds in the colloid solution obtained by the secondary dispersion was about 50 nm.

Example 3

[0060] In this example, a preparation method of monodisperse aminated Nanodiamond colloid solution are described as follows.

[0061] (1) The ball milling beads (a large agate bead diameter of 6.8 mm and a small bead diameter of 4.2 mm, mass ratio of 1:5) were put into a ball milling tank with the volume of 100 ml, to mix with 0.5 g purified Nanodiamond and 2.5 g chlorinated Sodium, 5 g ammonium chloride. The ball mill (model: QM-1SP2, Nanjing University Instrument Factory) with a linear speed of 512 m/min works for 2 h. Take off the ball mill tank after turning off the ball mill for 30 minutes.

[0062] (2) The ball milling beads in the ball milling tank were washed with deionized water (250300 ml, the volume of deionized water can be appropriately increased), and dispersed with ultrasonic (8000 W ultrasound generally does not exceed 1 min, 360 W ultrasound generally 15 min), and then centrifuged for 5 min with a speed of 10000 r/min, repeating 4 to 5 times to obtain clear and transparent monodisperse aminated Nanodiamond black colloid solution, which was characterized by dynamic light scattering (DLS), and the results were shown in FIG. 4.

[0063] In this example, a secondary dispersion technology of monodisperse ammoniated Nanodiamond colloid solution are described as follows.

[0064] (1) Water solvent in the clear and transparent Nanodiamond black colloid solution was removed with a rotary evaporator (model: N-1001, Shanghai Ailang Instrument Co., Ltd.), and the temperature of water bath (model: SB-2000, Shanghai Ailang Instrument Co., Ltd.) was 80 C. In this way, aminated Nanodiamond powder was obtained.

[0065] (2) The aminated Nanodiamond powder was dissolved in DMSO by ultrasonic for 15 minutes to obtain clear and transparent black colloid solution of Nanodiamond with higher concentration.

[0066] The aminated Nanodiamond powder was tested by XRD. The test result was shown in FIG. 1. The XRD diffraction peaks of the aminated Nanodiamond powder and the Nanodiamond raw material and purified Nanodiamond were basically the same, which proved that the structure of the Nanodiamond after amination modification was basically unchanged.

[0067] The colloid solution after secondary dispersion was characterized by dynamic light scattering, and the result was shown in FIG. 7. The median size of the aminated Nanodiamonds in the colloid solution prepared in this example was about 50 nm, and the average particle size of the aminated Nanodiamonds in the colloid solution obtained by the secondary dispersion was about 60 nm.

Example 4

[0068] In this example, a preparation method of monodisperse aminated Nanodiamond colloid solution are described as follows.

[0069] (1) The ball milling beads (a large agate bead diameter of 6.8 mm and a small bead diameter of 4.2 mm, mass ratio of 1:5) were put into a ball milling tank with the volume of 100 ml, to mix with 0.5 g purified Nanodiamond and 2.5 g chlorinated Sodium, 7.5 g ammonium chloride. The ball mill (model: QM-1SP2, Nanjing University Instrument Factory) with a linear speed of 512 m/min works for 2 h. Take off the ball mill tank after turning off the ball mill for 30 minutes.

[0070] (2) The ball milling beads in the ball milling tank were washed with deionized water (250300 ml, the volume of deionized water can be appropriately increased), and dispersed with ultrasonic (8000 W ultrasound generally did not exceed 1 min, 360 W ultrasound generally 15 min), and then centrifuged for 5 min with a speed of 10000 r/min, repeating 4 to 5 times to obtain clear and transparent monodisperse aminated Nanodiamond black colloid solution, which was characterized by dynamic light scattering (DLS), and the results were shown in FIG. 4.

[0071] In this example, a secondary dispersion technology of monodisperse ammoniated Nanodiamond colloid solution are described as follows.

[0072] (1) Water solvent in the clear and transparent Nanodiamond black colloid solution was removed with a rotary evaporator (model: N-1001, Shanghai Ailang Instrument Co., Ltd.), and the temperature of water bath (model: SB-2000, Shanghai Ailang Instrument Co., Ltd.) was 80 C. In this way, aminated Nanodiamond powder was obtained.

[0073] (2) The aminated Nanodiamond powder was dissolved in ethanol by ultrasonic for 15 minutes to obtain clear and transparent black colloid solution of Nanodiamond with higher concentration, which was characterized by dynamic light scattering, and the result was shown in FIG. 7. The median size of the aminated Nanodiamonds in the colloid solution prepared in this example was about 50 nm, and the average particle size of the aminated Nanodiamonds in the colloid solution obtained by the secondary dispersion was about 60 nm.

Example 5

[0074] In this example, a preparation method of monodisperse aminated Nanodiamond colloid solution are described as follows.

[0075] (1) The ball milling beads (a large agate bead diameter of 6.8 mm and a small bead diameter of 4.2 mm, mass ratio of 1:5) were put into a ball milling tank with the volume of 100 ml, to mix with 0.5 g purified Nanodiamond and 2.5 g chlorinated Sodium, 10.0 g ammonium chloride. The ball mill (model: QM-1SP2, Nanjing University Instrument Factory) with a linear speed of 512 m/min works for 2 h. Take off the ball mill tank after turning off the ball mill for 30 minutes.

[0076] (2) The ball milling beads in the ball milling tank were washed with deionized water (250300 ml, the volume of deionized water can be appropriately increased), and dispersed with ultrasonic (8000 W ultrasound generally did not exceed 1 min, 360 W ultrasound generally 15 min), and then centrifuged for 5 min with a speed of 10000 r/min, repeating 4 to 5 times to obtain clear and transparent monodisperse aminated Nanodiamond black colloid solution, which was characterized by dynamic light scattering (DLS), and the results were shown in FIG. 4.

[0077] In this example, a secondary dispersion technology of monodisperse ammoniated Nanodiamond colloid solution are described as follows.

[0078] (1) Water solvent in the clear and transparent Nanodiamond black colloid solution was removed with a rotary evaporator (model: N-1001, Shanghai Ailang Instrument Co., Ltd.), and the temperature of water bath (model: SB-2000, Shanghai Ailang Instrument Co., Ltd.) was 80 C. In this way, aminated Nanodiamond powder was obtained.

[0079] (2) The aminated Nanodiamond powder was dissolved in ethanol by ultrasonic for 15 minutes to obtain clear and transparent black colloid solution of Nanodiamond with higher concentration.

[0080] FIG. 1 shows a XRD patterns of Nanodiamond gray powder and purified Nanodiamond. From the XRD patterns of unpurified raw material of Nanodiamond, it can be observed that the peak at 2030 degree appearing in (a) is showing a peak of impurities, such as graphite (100) and amorphous carbon, etc., and the noise is relatively high. This shows that the unpurified Nanodiamond crystal form is defective and contains certain content of non-diamond carbon. From the XRD patterns of Nanodiamond oxidized by potassium permanganate (b), it can be observed that the peak at 2030 degree is disappeared, indicating that potassium permanganate has good impurity removal effect on amorphous carbon and graphite. Nanodiamond powder with high purity and perfect crystal shape is obtained through oxidation treatment. In addition, due to the strong oxidation of potassium permanganate, Nanodiamonds are etched, which reduces the carbon content of (111) and (220) crystal forms. From the XRD patterns of Nanodiamond modified by ball milling and amination in example 1 (c), it can be observed that the (111) crystal face and (220) crystal face of the nanodiamond modified by amination are complete, and the strength does not change much from that of the purified Nanodiamond. This shows that ball mill amination modification has no significant effect on the Nanodiamond crystal form, and the integrity of the Nanodiamond crystal form is maintained in the amination process.

[0081] FIG. 2 shows a FTIR spectrum of the Nanodiamond sample prepared in example 1 purified (a) and after amination (b).

[0082] It can be seen from the FTIR spectrogram that carboxyl groups are attached to the surface of the purified Nanodiamond. The broad absorption peak near 3432 cm.sup.1 in the figure is OH stretching vibration absorption peak, and the absorption peak near 1629 cm.sup.1 is OH bending vibration absorption peak, COOH stretching vibration absorption peak near 1791 cm.sup.1, which indicates that carboxyl and hydroxyl groups are attached to the surface of the oxidized Nanodiamond. The COC antisymmetric stretching vibration peak appears near 1259 cm.sup.1. In the spectrum after amination modification, the broad absorption peak near 3413 cm.sup.1 is a stretching vibration peak of NH, and the new absorption peak appearing at 1401 cm.sup.1 is a stretching vibration peak of CN. This characteristic peak indicates that the surface of Nanodiamond modified by ball milling is bonded with amino groups. Comparing the graphs of a and b, the carbonyl stretching vibration peak of Nanodiamonds after amination modification is red-shifted from 1791 cm.sup.1 to around 1779 cm.sup.1, which also indicates that Nanodiamonds can be efficiently functionalized by ball milling amine. The modified Nanodiamond is successfully obtained.

TABLE-US-00001 TABLE 1 Infrared spectrum analysis FTIR PEAK WAVE NUMBER/CM.sup.1 CORRESPONDING VIBRATION PEAK 3413 a stretching vibration peak of NH 1791 a stretching vibration absorption peak of CO 1629 a bending vibration absorption peak of OH 1401 a stretching vibration peak of CN

[0083] FIG. 3 shows a photo of Nanodiamond colloid solution in different amination reagent ratio conditions of example 1 to 5. FIG. 4 shows a particle size distribution of Nanodiamond colloid solution in different amination reagent ratio (The mass ratio of Nanodiamond to ammonium chloride) conditions of example 1 to 5. It can be observed that while the mass ratio of amination reagent to Nanodiamond is 1:0, the dispersion effect of Nanodiamond is poor, and the aqueous solution is an emulsion with an average particle size of 516 nm. After adding aminated modified particles, the Nanodiamond dispersion effect is better. While the amount of amination reagent increased from 1:5 to 1:10, the smallest particle size of Nanodiamonds reached 43.4 nm. The surface of the aminated Nanodiamond has more hydrophilic groups, which improves the dispersibility of the Nanodiamond in deionized water; the immobilization of the amino group on the surface of the Nanodiamond can enhance the stability of the system. Therefore, the modified Nanodiamond can be stored stably for a long time.

[0084] FIG. 5 shows a Zeta potential-pH graph of Nanodiamond colloid solution in different amination reagent ratio conditions of example 1 to 5. It can be observed that while the ratio of amination reagent is 1:10, the degree of potential decrease is the most significant. The isoelectric point appears around pH=3. The potential value corresponding to different pH is the smallest. While pH=10, the minimum zeta potential is 49.9 mV. While the ratio of aminating reagent increases from 1:0 to 1:10, the corresponding lower potential value of each pH decreases, and while the ratio of aminating reagent increases to 1:20, the corresponding potential value of each pH value increases. These indicate that more hydrophilic groups are attached to the surface of Nanodiamonds after amination modification, and the particles have greater electrostatic repulsion, so as to achieve the dispersion effect.

[0085] FIG. 6 shows a photo of the colloid solution of aminated nanodiamond prepared in example 1 for secondary dispersion in different solvents. It can be seen that after amination modification, Nanodiamonds are dispersed in aqueous phase to obtain colloid solution, and Nanodiamond particles are obtained after the colloid solution is dried. The particles can be dispersed again in the solvent to form colloids with an ultrasonic, such as deionized water, DMSO, ethanol, DMF, etc. DLS test results show that the average particle size of Nanodiamonds has little change and can be stored stably for a long time, so it can be widely used in drug carriers, thermal interface materials, photoelectric materials, and lubricating materials. Experimental conditions: grinding for 2 hours, grinding media ratio of 1:5, grinding material ratio of 1:10, grinding aid ratio (NaCl) of 1:5.

[0086] FIG. 7 shows a particle size distribution of the colloid solution of aminated Nanodiamond prepared in example 3 for secondary dispersion in different solvents. FIG. 8 shows a TEM images of aminated Nanodiamond prepared in example 3. It can be seen that the Nanodiamond modified by amination can be dispersed in deionized water, ethanol, DMSO, and DMF again, and present colloid state. The analysis of the DLS results show that a average particle size of the re-dispersed water is 48.9 nm, 53.6 nm in ethanol, 58.4 nm in DMSO, and 68.7 nm in DMF. All particle sizes are below 100 nm, indicating that the aminated modified Nanodiamonds have better re-dispersion effect.

Example 6 Application of the Aminated Nanodiamonds Prepared in Example 1 as Fluorescent Probes in Cellular Biomarking

[0087] 1, Cell culture and labeling

[0088] (1) Five types of cells were respectively seeded on a six-well cell culture plate, the cell density in each well: 510.sup.50.0510.sup.5 cells, cultured in an incubator with 37 C., 5% CO.sub.2 for 24 hours. According to three different dyeing times required by the experiment, two parallel experiments were set up for each dyeing time.

[0089] (2) A mixed dye solution of aminated Nanodiamond and culture medium was prepared, and a concentration of aminated Nanodiamond was 1 mg/ml.

[0090] (3) The cultured six-well cell culture plate was taken out, the medium in it was aspirated and discarded, and 3 ml of the mixed solution was added to each well for cell staining. Continue to cultivate.

[0091] 2, Collect cell marker pictures

[0092] (1) After adding the aminated Nanodiamond mixture, the mixture was sucked out at 5 min, 15 min, and 30 min.

[0093] (2) After that, the cells were washed with PBS for three times, and 1 ml of fresh medium was added to each well of the six-well cell culture plate.

[0094] (3) Observed with microscope, the pictures of bright field and fluorescence field in the same field of view were collected separately.

[0095] 3, FIG. 9A-E shows a real-time photographs of aminated Nanodiamond entering living cells. Comparing the fluorescence intensity of the real-time taken pictures of aminated Nanodiamond staining for 30 minutes with the previous outside of the cell, it can be found that the fluorescence intensity of the aminated Nanodiamond particles is relatively weak before entering the cell, but the intensity is increased in the real-time taken picture. The reason may be that the density of aminated Nanodiamonds increased after entering the cells, and the aggregation effect is achieved in the cells, the intracellular vibration is restricted, the radiation attenuation ratio increased, and the fluorescence intensity increased. Therefore, aminated Nanodiamonds can be used as fluorescent probes to label cells.

[0096] Although the aminated Nanodiamond have fluorescence intensity, its intensity is not obvious. After the aminated Nanodiamond enters the cell, since the surface of the aminated Nanodiamond particle is attached with many amino functional groups, as the cell absorbs the particles, the distance between the particles in the cell will gradually decrease to form many hydrogen bonds. Because the structure of aminated Nanodiamonds is relatively stable, it did not perform intramolecular motion and rotation like ordinary organic molecules to release energy to return the molecules to steady state, but may release energy in weak vibration mode. However, the higher energy released by the vibration mode is suppressed due to the formation of hydrogen bonds, making it return to steady state. Energy release in the form of light energy is increased, and fluorescence intensity is increased.