Synthesis of human milk oligosaccharides by a Beta-N-acetylhexosaminidase from Haloferula sp.

Abstract

The invention discloses the application of a β-N-acetylhexosaminidase (HaHex74) from Haloferula sp. in the synthesis of human milk oligosaccharides. The invention provides the use of HaHex74 protein or related biological materials thereof in any one of the following: synthesizing human milk oligosaccharides; synthesizing Lacto-N-triose II and/or Lacto-N-neotetraose; the HaHex74 protein having the amino acid sequence shown in SEQ ID No. 2 is derived from Haloferula sp. The β-N-acetylhexosaminidase HaHex74 disclosed by the invention possesses high-level expression, excellent hydrolysis properties and transglycosylation activity, which may make it potentially useful in the production of human milk oligosaccharides.

Claims

1-31. (canceled)

32. A method for the synthesis of Lacto-N-triose II comprise the following steps (b1) or (b2) or (b3): (b1) synthesizing Lacto-N-triose II (LNT2) from chitin hydrolysate and β-lactose under the catalysis of a HaHex74 protein as a biological enzyme; (b2) performing chitin hydrolysis catalyzed by a chitinase to obtain the chitin hydrolysate; then synthesizing Lacto-N-triose II (LNT2) from the chitin hydrolysate and β-lactose under the catalysis of the HaHex74 protein; and (b3) synthesizing Lacto-N-triose II (LNT2) from the chitin hydrolysate and β-lactose under the catalysis of the chitinase and HaHex74 protein as biological enzymes; the HaHex74 protein is any one of the following: (A1) a protein comprising an amino acid sequence at positions 94-745 of SEQ ID No.2 or shown in SEQ ID No.2; (A2) a protein comprising an amino acid sequence including substitution and/or deletion and/or addition of one or several amino acid residues in the amino acid sequence at positions 94-745 of SEQ ID No.2 or shown in SEQ ID No.2 and having the same function; (A3) a protein having 99% or more, 95% or more, 90% or more, 85% or more or 80% or more homology with the amino acid sequence defined in any one of (A1)-(A2) and having the same function; and (A4) a fusion protein obtained by attaching a tag to the N-terminus and/or the C-terminus of the protein defined in any one of (A1)-(A3).

33. A method for the synthesis of Lacto-N-neotetraose comprise the following steps (c1) or (c2): (c1) preparing Lacto-N-triose II (LNT2) according to the method of the second aspect; then synthesizing Lacto-N-neotetraose (LNnT) from Lacto-N-triose II (LNT2) and β-lactose under the catalysis of a β-galactosidase; and (c2) synthesizing Lacto-N-triose II (LNT2) and Lacto-N-neotetraose (LNnT) from chitin and β-lactose under the catalysis of a chitinase, HaHex74 protein and β-galactosidase as biological enzymes, the HaHex74 protein is any one of the following: (A1) a protein comprising an amino acid sequence at positions 94-745 of SEQ ID No.2 or shown in SEQ ID No.2; (A2) a protein comprising an amino acid sequence including substitution and/or deletion and/or addition of one or several amino acid residues in the amino acid sequence at positions 94-745 of SEQ ID No.2 or shown in SEQ ID No.2 and having the same function; (A3) a protein having 99% or more, 95% or more, 90% or more, 85% or more or 80% or more homology with the amino acid sequence defined in any one of (A1)-(A2) and having the same function; and (A4) a fusion protein obtained by attaching a tag to the N-terminus and/or the C-terminus of the protein defined in any one of (A1)-(A3).

34. The method according to claim 32, wherein, in the (b1) and the (b2), the chitin hydrolysate is a mixture of N-acetyl chitobiose and N-acetylglucosamine.

35. The method according to claim 32, wherein, Lacto-N-triose II (LNT2) is synthesized from chitin hydrolysate and β-lactose catalyzed by the HaHex74 protein as a biological enzyme under conditions of pH of 7.5, and/or a temperature of 40° C., and/or a content of β-lactose in the reaction system of 0.8 M; and/or a content of the HaHex74 protein in the reaction system of 3-5 U/mL (such as 4 U/mL), and/or a reaction time of 10 h.

36. The method according to claim 32, wherein, chitin hydrolysate is obtained from the hydrolysis of chitin catalyzed by the chitinase under conditions of pH 5.5, and/or a temperature of 55° C., and/or a content of the chitinase in a reaction system of 5 U/mL, and/or a reaction time of 24 h.

37. The method according to claim 32, wherein, Lacto-N-neotetraose (LNnT) is synthesized from Lacto-N-triose II (LNT2) and β-lactose catalyzed by a β-galactosidase under conditions of natural pH (pH 7.0), and/or a temperature of 50° C., and/or a content of β-galactosidase in the reaction system of 0.1 U/mL, and/or a reaction time of 10 h.

38. The method according to claim 32, wherein, the HaHex74 protein is prepared according to a method comprising the steps of introducing a nucleic acid molecule encoding the HaHex74 protein into a yeast receptor to obtain recombinant yeast; performing fermentation culture on the recombinant yeast according to the following steps to obtain the HaHex74 protein from a fermentation product: (d1) a basic culture stage: inoculating the recombinant yeast into BSM medium supplemented with 50 g/L glycerol for culture, adjusting a temperature to 30° C. and pH to 4.0,and starting the glycerol fed-batch culture stage when the glycerol concentration is below 10 g/L; (d2) a glycerol fed-batch culture stage: feeding 500 g/L glycerol, maintaining the glycerol concentration at 10-25 g/L, adjusting a temperature to 28° C., pH to 5.0, and the dissolved oxygen content to 10-20% until the end of fermentation.

39. The method according to claim 38, wherein, obtaining the HaHex74 protein from the fermentation product is performed according to a process comprising the steps of centrifuging the fermentation product to collect supernatant; dialyzing the supernatant in a Tris-HCl buffer solution, and centrifuging to obtain crude enzyme; purifying the crude enzyme using a (diethylamino)ethyl DE52 column (DEAE Sepharose Fast Flow) to obtain the HaHex74 protein

40. The method according to claim 38, wherein, the nucleic acid molecule encoding the HaHex74 protein may be a DNA molecule as described in any one of the following: (B1) a DNA molecule represented by positions 280-2238 of SEQ ID No. 3 or SEQ ID No. 3; (B2) a DNA molecule hybridizing with the DNA molecule defined by b1) or b2) under stringent conditions and encoding the HaHex74 protein; (B3) a DNA molecule having 99% or more, 95% or more, 90% or more, 85% or more or 80% or more homology with the DNA sequence defined by (B1) or (B2) and encoding the HaHex74 protein.

41. The method according to claim 38, wherein, the nucleic acid molecule may be introduced into the yeast receptor in the form of a recombinant vector; wherein, the promoter in the recombinant vector that initiates transcription of the nucleic acid molecule may be a GAP promoter.

42. The method according to claim 41, wherein, the sequence of the GAP promoter is shown in SEQ ID No.1.

43. The method according to claim 38, wherein, the yeast is Pichia pastoris.

44. The method according to claim 43, wherein the Pichia pastoris is Pichia pastoris GS115.

45. A preparation method of HaHex74 protein, may comprise the following steps: introducing a nucleic acid molecule encoding the HaHex74 protein into a yeast receptor to obtain recombinant yeast; performing fermentation culture on the recombinant yeast according to the following steps to obtain the HaHex74 protein from a fermentation product: (d1) a basic culture stage: inoculating the recombinant yeast into BSM medium supplemented with 50 g/L glycerol for culture, adjusting the temperature to 30° C. and pH to 4.0, and starting the glycerol fed-batch culture stage when the glycerol concentration is below 10 g/L. (d2) a glycerol fed-batch culture stage: feeding 500 g/L glycerol, maintaining the glycerol concentration at 10-25 g/L, adjusting a temperature to 28° C., pH to 5.0, and the dissolved oxygen content to 10-20% until the end of fermentation; the HaHex74 protein is any one of the following: (A1) a protein comprising an amino acid sequence at positions 94-745 of SEQ ID No.2 or shown in SEQ ID No.2; (A2) a protein comprising an amino acid sequence including substitution and/or deletion and/or addition of one or several amino acid residues in the amino acid sequence at positions 94-745 of SEQ ID No.2 or shown in SEQ ID No.2 and having the same function; (A3) a protein having 99% or more, 95% or more, 90% or more, 85% or more or 80% or more homology with the amino acid sequence defined in any one of (A1)-(A2) and having the same function; and (A4) a fusion protein obtained by attaching a tag to the N-terminus and/or the C-terminus of the protein defined in any one of (A1)-(A3).

46. The preparation method according to claim 45, wherein, obtaining the HaHex74 protein from the fermentation product may be performed according to a process comprising the steps of centrifuging the fermentation product to collect supernatant; dialyzing the supernatant in a Tris-HCl buffer solution, and centrifuging to obtain crude enzyme; purifying the crude enzyme using a (diethylamino)ethyl DE52 column (DEAE Sepharose Fast Flow) to obtain the HaHex74 protein.

47. The preparation method according to claim 45, wherein, the nucleic acid molecule encoding the HaHex74 protein may be a DNA molecule as described in any one of the following: (B1) a DNA molecule represented by positions 280-2238 of SEQ ID No. 3 or SEQ ID No. 3; (B2) a DNA molecule hybridizing with the DNA molecule defined by b1) or b2) under stringent conditions and encoding the HaHex74 protein; (B3) a DNA molecule having 99% or more, 95% or more, 90% or more, 85% or more or 80% or more homology with the DNA sequence defined by (B1) or (B2) and encoding the HaHex74 protein.

48. The preparation method according to claim 45, wherein, the nucleic acid molecule may be introduced into the yeast receptor in the form of a recombinant vector; wherein, the promoter in the recombinant vector that initiates transcription of the nucleic acid molecule may be a GAP promoter.

49. The preparation method according to claim 48, wherein, the sequence of the GAP promoter is shown in SEQ ID No.1.

50. The preparation method according to claim 45, wherein, the yeast is Pichia pastoris.

51. The preparation method according to claim 50, wherein the Pichia pastoris is Pichia pastoris GS115.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0083] FIG. 1 Time course of HaHex74 expressed in P. pastoris (A) and SDS-PAGE analysis of extracellular proteins (B). ((.square-solid.): enzyme activity; (.box-tangle-solidup.): protein concentration; (.circle-solid.): wet weight).

[0084] FIG. 2 SDS-PAGE analysis of HaHex74 before and after purification.

[0085] FIG. 3 Optimal pH (A), pH stability (B), optimal temperature (C) and thermostability (D) of HaHex74. Wherein, (.square-solid.) citric acid buffer (pH 5.0-6.0), (.circle-solid.) acetic acid-sodium acetate buffer (pH 4.0-6.0), (.box-tangle-solidup.) MES buffer (pH 5.5-6.5), (.Math.) MOPS buffer (pH 6.5-7.5), (.diamond-solid.) phosphate buffer (pH 6.0-8.0), () Tris-HCl buffer (pH 7.0-9.0), () glycine-sodium hydroxide buffer (pH 8.5-10.5).

[0086] FIG. 4 Optimal pH (A), optimal temperature (B), optimal reaction time (C), optimal β-lactose concentration (D) and optimal enzyme dosage (E) for the synthesis of LNT2 by HaHex74. Wherein, (.square-solid.) MES buffer (pH 5.5-6.5), (.box-tangle-solidup.) phosphate buffer (pH 6.0-8.0) and (.Math.) Tris-HCl buffer (pH 7.0-9.0).

[0087] FIG. 5 MS spectrum of purified product synthesized by HaHex74.

[0088] FIG. 6 .sup.1H spectrum of purified product synthesized by HaHex74.

[0089] FIG. 7 .sup.13C spectrum of purified product synthesized by HaHex74.

[0090] FIG. 8 Heteronuclear singular quantum correlation (HSQC) spectrum of purified product synthesized by HaHex74.

[0091] FIG. 9 Time course of LNT2 production using chitin hydeolysate and lactose by HaHex74 (.square-solid.): conversion ratio; (.circle-solid.): LNT2 concentration).

[0092] FIG. 10 Time course of LNnT production (.square-solid.): conversion ratio; (.circle-solid.): LNnT concentration).

EMBODIMENTS

[0093] The following examples facilitate a better understanding of the present invention but do not limit the present invention. The experimental methods in the following examples are conventional unless otherwise specified. The experimental materials used in the following examples were purchased from conventional biochemical reagent stores unless otherwise specified. In the quantitative experiments of the following examples, triplicate experiments are set up, and the results are averaged.

[0094] The enzyme activity of β-N-acetylhexosaminidase in the following examples was determined as follows: 0.1 mL of appropriately diluted enzyme solution was added to 0.1 mL of a 2 mM pNP GlcNAc substrate solution (prepared in 50 mM phosphate buffer, pH 6.5), and the mixture was reacted at 45° C. for 10 min to determine the absorbance at 410 nm (OD.sub.410) (Yang et al. Biochemical characterization of the first fungal glycoside hydrolyase family 3 β-N-acetylglucosaminidase from Rhizomucor michei. J. Agric. Food Chem., 2014, 62, 5181-5190).

[0095] A standard enzyme-activity unit of β-N-acetylhexosaminidase is given below: one unit (1 U) is the amount of enzyme required to produce 1 μmol ofpNP per minute under the above reaction conditions.

[0096] Specific enzyme activity is defined as the unit of enzyme activity per 1 mg of protein, expressed as U.Math.mg.sup.−1.

[0097] One unit of β-N-acetylhexosaminidase activity is defined as: under the conditions of pH 6.5, 45° C., the amount of enzyme required to release 1 μmol of pNP per minute by decomposing 2 mM pNP-GlcNAc substrate solution (formulated in 50 mM phosphate buffer, pH 6.5) and is calculated as follows: H=Cx×n/(T×V), wherein H represents the enzyme activity (U/mL), Cx represents the amount of pNP produced (μmol), n represents the dilution multiple of the enzyme solution, T represents the reaction time (min), and V represents the volume of enzyme solution (mL) after addition of the dilution.

EXAMPLE 1

Identification of β-N-acetylhexosaminidase Gene and Construction of Engineered P. pastoris GS115

[0098] 1. Identification of β-N-acetylhexosaminidase Gene

[0099] The reported protein sequence of β-N-acetylhexosaminidase was searched by CAZy database, and the homologous sequence alignment analysis was performed to obtain a GH family 20 β-N-acetylhexosaminidase gene (named HaHex74) from Haloferula sp. The nucleotide sequence of the HaHex74 gene is “ATG+positions 280-2238 of SEQ ID No.3”, and encodes the amino acid sequence shown as “Met+positions 94-745 of SEQ ID No.2”. HaHex74 shared the highest (61%) homology with a soil metagenomic β-N-acetylhexosaminidase (AKC34129). The gene was synthesized by Sangon Biotech (Shanghai) Co., Ltd.

[0100] 2. Construction of Engineered P. pastoris GS115 for the β-N-acetylhexosaminidase Expression

[0101] 2.1 Primers were Designed According to a Glyceraldehyde-3-phosphate Dehydrogenase (GAP) Promoter Gene Sequence in the Pichia pastoris GS115 Genome:

TABLE-US-00001 GAP1: 5′-GCAGCGAGCTCATCCTTTTTTGTAGAAATGTCTTGG-3′; GAP2: 5′-CGCGGATCCTGTGTTTTGATAGTTGTTCAATTGA-3′.

[0102] The GAP promoter was amplified from the Pichia pastoris GS115 genome. The GAP promoter fragment and pPIC9K vector were subjected to double digestion with Sac I and BamHI and ligated by T4 DNA ligase to obtain the modified expression vector, named pGAP9K. That is, the AOX1 promoter in the pPIC9K vector was replaced with the GAP promoter. Wherein, the GAP promoter sequence is shown in SEQ ID No.1.

[0103] 2.2 Expression Primers were Designed According to the Sequence of β-N-acetylhexosaminidase, EcoRI and NotI Restriction Sites were Respectively Added to the Following Upstream Primer and the Downstream Primer:

TABLE-US-00002 Upstream primer: 5′-CCGGAATTCGAACCAACCATTATTCCATTGCC-3′; Downstream primer: 5′-AGAATGCGGCCGCTTACTCAACGGTGATTTCGTGGATA-3′

[0104] The PCR amplification was performed with the above primers, and the conditions were as follows: 95° C. for 30 s; 95° C. for 20 s, 58° C. for 20 s, 72° C. for 1 min followed by 34 cycles; and final extension at 72° C. for 5 min. PCR products were recovered by 1% agarose gel electrophoresis and subjected to double digestion with EcoRI and NotI. The double restriction enzyme digested products were ligated with a vector backbone fragment of a yeast expression vector pGAP9K which was subjected to the same double digestion by T4 DNA ligase, and was then transformed into Escherichia coli DH5α. The recombinant expression vector verified by sequencing was designated as pGAP9K-HaHex74. The obtained recombinant P pastoris expression vector pGAP9K-HaHex74 was subjected to linearization by a restriction endonuclease XbaI, and transformed into P. pastoris GS115 by electroporation.

[0105] The GAP promoter sequence in the recombinant expression vector pGAP9K-HaHex74 is the DNA molecule in SEQ ID No.1 (i.e. constructing a new expression vector pGAP9K by replacing the AOX1 promoter in the pPIC9K vector with the GAP promoter through double digestion with Sac I and BamH I); the complete ORF sequence of the recombinant expression vector pGAP9K-HaHex74 is a DNA molecule in SEQ ID No.3, wherein positions 1-279 are sequences on the pGAP9K vector, and positions 280-2238 are HaHex74 gene sequences.

EXAMPLE 2

Preparation of the β-N-acetylhexosaminidase (HaHex74) and its Enzymatic Properties

[0106] 1. Expression of the β-N-acetylhexosaminidase in P. pastoris GS115

[0107] The recombinant strains obtained in Example 1 were spread on MD plates (1.34% YNB, 4×10.sup.−5% biotin, 2% glucose), His.sup.+ transformants obtained from MD plates were scraped with sterile water, and 100 μL of transformants was spread on YPD-G418 plates (1% yeast extract, 2% tryptone, 2% glucose, G418 concentrations of 2, 3, 4, 6 mg/mL, respectively) at different concentrations. After incubation for 3-5 d at 30° C., transformants were picked out and cultured in BMGY medium for 16-18 h, followed by centrifuging at 3000 rpm for 5 min. Cells were collected, then resuspended with the BMGY medium until the OD.sub.600 was about 1.0, and glycerol was fed in batches to express the target protein. The above mixture was cultured in 20 mL medium in a 100 mL triangular flask at 30° C. and 200 rpm. Glycerol was added every 24 h to a final concentration of 1% for 3 d, the enzyme activity of the fermentation broth was determined, and the expression of HaHex74 recombinant protein was analyzed by SDS-PAGE.

[0108] 2. High Cell Density Fermentation

[0109] The strain with the highest enzyme activity obtained in step 1 of Example 2 was subjected to high-density fermentation in a 5-L fermenter. The medium used during the fermentation (seed medium BMGY, fermentation minimal medium BSM and glycerol fed-batch medium) was performed according to Pichia Fermentation Process Guidelines (Version B, 053002, Invitrogen). The whole fermentation process adopted two stages of basic culture and glycerol fed-batch culture.

[0110] (1) Seed culture: the strain with the highest enzyme activity in flask shaking fermentation was inoculated into 150 mL BMGY medium and cultured at 30° C. and 200 rpm until the OD.sub.600 was about 10.0.

[0111] (2) Basic culture: the seed solution obtained in step (1) was inoculated into a 5-L fermenter (containing 1.35 L of a fermentation basic medium BSM and 5% (50 g/L) of glycerol, the fermenter was sterilized, the pH was adjusted to 4.0 by 28% concentrated ammonia water, and an initial fermentation broth with 4.35 mL/L of PTM1 (trace elements known in the art for promoting yeast expression) was added, with an inoculum volume of 10% (v/v), a rotation speed of 600 rpm, and a temperature of 30° C. When the glycerol concentration was below 1% (10 g/L), 50% (w/v) glycerol (500 g/L) feed medium was fed in batches.

[0112] (3) Fed-batch culture with glycerol: 50% (w/v) glycerol (500 g/L) was fed in batches under conditions of 28° C., pH 5.0, and a rotation speed of 800 rpm, a glycerol concentration was maintained at 1%-2.5% (10 g/L-25 g/L), the dissolved oxygen was constantly monitored, and the rotation speed and the ventilation volume were timely adjusted to maintain the DO at 10%-20% until the fermentation end-point.

[0113] The wet weight, protein content and enzyme activity were determined during fermentation. The results showed that the highest enzyme activity and protein concentration were 3500 U/mL and 9.7 g/L, respectively after 168-h fermentation (FIG. 1).

[0114] 3. Purification of β-N-acetylhexosaminidase

[0115] After the high-density fermentation, the fermentation broth was centrifuged at 10,000 g for 5 min, the supernatant was collected, and 50 mL of the supernatant was dialyzed in Tris-HCl buffer solution (20 mM, pH 8.0) overnight. The dialysate was centrifuged at 10,000 g for 5 min to obtain a crude enzyme solution. The crude enzyme solution was purified by a (diethylamino)ethyl DE52 column (DEAE Sepharose Fast Flow) to obtain purified HaHex74. Specific steps were as follows:

[0116] The DE52 column was equilibrated with Buffer A for 5-10 column volumes, the collected enzyme solution was loaded at a flow rate of 0.5 mL/min. Buffer A was eluted linearly with Buffer B to an OD 280 <0.05 using a protein purification system (ÄKTA, GE Healthcare, USA) (elution procedure: 0-30 min, A 100%; 30-120 min, A 100%-0, B 0-100%; 120-135 min, B 100%; % denotes volume percent). Fractions with β-N-acetylhexosaminidase activity were collected, checked for purity by SDS-PAGE, and dialyzed to obtain purified product.

[0117] Wherein buffer A was a buffer solution containing 20 mM Tris-HCl (pH 8.0); buffer B was 20 mM Tris-HCl buffer (pH 8.0) containing NaCl (500 mM).

[0118] The purified product obtained from the crude enzyme solution of recombinant strain was the recombinant protein HaHex74.

[0119] The SDS-PAGE of the crude enzyme solution of and the resulting purified product (recombinant protein HaHex74) were shown in FIG. 2, in which lane M represented a molecular weight protein standard, lane 1 represented the recombinant protein HaHex74 crude enzyme, and lane 2 represented the HaHex74 pure enzyme. FIG. 2 showed that the molecular weight of the recombinant protein HaHex74 was approximately 74 kDa, consistent with the expected size. The enzyme activity of β-N-acetylhexosaminidase was detected, with the crude enzyme solution and the purified enzyme solution as enzyme solutions to be detected respectively, and the corresponding inactivated protein as a control. The total enzyme activity of the crude enzyme solution shown in Table 1 was 3500 U, and the specific enzyme activity was 360.8 U mg.sup.−1; the total enzyme activity of purified enzyme solution was 2890 U, and its specific enzyme activity was 385.3 U mg.sup.−1; the purification fold was 1.1.

TABLE-US-00003 TABLE 1 Purification of β-N-acetylhexosaminidase HaHex74 Total Specific enzyme enzyme Recovery Purification activity Total activity Purification rate Step (U) Protein (U mg.sup.−1) fold (%) Crude 3500 9.7 360.8 1 100 enzyme solution DE52 2890 7.5 385.3 1.1 82.6

[0120] 4. Enzymatic Properties of β-N-acetylhexosaminidase

[0121] (1) Determination of Optimal pH

[0122] The purified HaHex74 was used to be detected, and enzyme activity was determined under 40° C. in different buffer solution systems respectively, and the relative enzyme activity was calculated by taking the highest enzyme activity as reference (100%). The various buffers were as follows:

[0123] 1) Citric acid buffer (pH 5.0-6.0)

[0124] 2) Acetic acid-sodium acetate buffer (pH 4.0-6.0)

[0125] 3) MES buffer (pH 5.5-6.5)

[0126] 4) MOPS buffer (pH 6.5-7.5)

[0127] 5) Phosphate buffer (pH 6.0-8.0)

[0128] 6) Tris-HCl buffer (pH 7.0-9.0)

[0129] 7) Glycine-sodium hydroxide buffer (pH 8.5-10.5).

[0130] The results were shown in FIG. 3A: the optimal pH of HaHex74 was pH 6.5.

[0131] (2) Determination of pH Stability

[0132] HaHex74 was diluted with the above buffer, incubated at 35° C. for 30 min, rapidly cooled in ice water for 30 min, and then assayed for the enzyme activity. The relative enzyme activity of HaHex74 processed under different pH was calculated, taking the enzyme activity of untreated β-N-acetylhexosaminidase as reference (100%).

[0133] The results were shown in FIG. 3B: HaHex74 had good pH stability, and more than 80% enzyme activity remained after 30 min incubation at pH 5.5-9.0.

[0134] (3) Determination of Optimal Temperature

[0135] HaHex74 was appropriately diluted with 50 mM phosphate buffer (pH 6.5), and enzyme activity was measured at different temperatures (20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70° C.), respectively. The relative enzyme activity was calculated, taking the highest enzyme activity as reference (100%).

[0136] The results were shown in FIG. 3C: the optimal temperature of HaHex74 was 45° C.

[0137] (4) Determination of Thermostability

[0138] HaHex74 was appropriately diluted with 50 mM phosphate buffer (pH 6.5), incubated at different temperatures (20, 25, 30, 35, 40, 45, 50, 55, 60° C.) for 30 min, rapidly cooled in ice water for 30 min, and then assayed for enzyme activity. The relative enzyme activity of HaHex74 untreated under different pH was calculated, taking the enzyme activity of untreated β-N-acetylhexosaminidase as reference (100%).

[0139] The results were shown in FIG. 3D: HaHex74 had good stability below 45° C.

[0140] (4) Substrate Specificity

[0141] Colloidal chitin, ethylene glycol chitin, carboxymethyl cellulose (CMC), chitosan solution, p-nitrophenyl-β-N-acetylglucosaminide (pNP-GlcNAc) and p-nitrophenyl-β-N-acetylgalactosamine (pNP-GalNAc) were used as substrates for the determination of enzyme activity and investigation of substrate specificity under the standard enzyme activity assay conditions. The results were shown in Table 2.

TABLE-US-00004 TABLE 2 Substrate specificity of the HaHex74 Specific enzyme Relative activity activity Substrates (U mg.sup.−1) (%) pNP-GlcNAc 385.3 ± 3.0 100 pNP-GalNAc  51.8 ± 1.8 13.4 (GlcNAc).sub.2   73.4 ± 1.0.sup.a 19.1 (GlcNAc).sub.3 112.8 ± 2.2 29.3 (GlcNAc).sub.4  54.3 ± 1.2 14.1 (GlcNAc).sub.5  44.2 ± 2.3 11.5 Colloidal chitin  0.14 ± 0.06 0.04 Ethylene glycol — — chitin Chitosan — — CMC — — Note: .sup.amethod for measuring enzyme activity towards N-acetyl chitooligosaccharides: 0.1 mL of appropriately diluted enzyme solution was added to 0.1 mL of 1% (w/v, 10 g/L) N-acetyl chitooligosaccharides solution (prepared in 50 mM phosphate buffer, pH 6.5) and reacted at 45° C. for 10 min. The amount of β-N-acetylglucosamine released was determined by high-performance liquid chromatography (HPLC) with β-N-acetylglucosamine as a standard. HPLC conditions were: HPLC-RID detection system (Agilent 1260 infinity II, Agilent Technologies, USA) BP-800 Pb++ chromatography column (Benson Polymeric, Reno, NE, 7.8 × 300 mm, USA), distilled water as mobile phase, column temperature 80° C., flow rate 1 mL .Math. min.sup.−1. A standard enzyme-activity unit of β-N-acetylaminoglucosidase is given below: one unit (1 U) is the amount of enzyme required to produce 1 μmol of β-N-acetylglucosamine per minute under the above reaction conditions.

[0142] The results showed that: HaHex74 showed the highest activity (385.3 U/mg) towards pNP GlcNAc; followed by N-acetyl chitotriose, N-acetyl chitobiose and N-acetyl chitotetraose. It showed weak activity towards colloidal chitin (0.14 U.Math.mg.sup.−1) and no activity towards glycol chitin, chitosan and CMC.

EXAMPLE 3

Optimization of Conditions for the Synthesis of LNT2

[0143] The amount of synthesized LNT2 was determined by HPLC. HPLC assay conditions were: Waters XBridge BEH Amide 5 μm column (250×4.6 mm), 72% acetonitrile, column temperature 45° C., flow rate 0.5 mL/min, 45 min, differential refraction detector (RID).

Transglycosylation product yield (%)=concentration of synthesized product (mM)/initial concentration of glycoside donor (mM)×100

[0144] 1. Determination of Optimal pH

[0145] The purified HaHex74 solution prepared in Example 2 was used as an enzyme solution to be detected and placed in different buffers respectively to determine a content of LNT2. The various buffers were as follows:

[0146] 1) MES buffer (pH 5.5-6.5)

[0147] 2) Phosphate buffer (pH 6.0-8.0)

[0148] 3) Tris-HCl buffer (pH 7.0-9.0)

[0149] The results were shown in FIG. 4A: the optimal pH for the synthesis of LNT2 by HaHex74 was pH 7.5.

[0150] 2. Determination of Optimal Temperature

[0151] The purified HaHex74 solution prepared in Example 2 was used as an enzyme solution to be tested, and the reaction system was subjected to water bath reaction at different temperatures (20-55° C.) for 1.5 h to determine a content of LNT2.

[0152] The results were shown in FIG.4B: the optimal temperature for the synthesis of LNT2 by HaHex74 was 40° C.

[0153] 3. Determination of Optimal Reaction Time

[0154] The purified HaHex74 solution prepared in Example 2 was used as an enzyme solution to be detected, and the reaction system was subjected to a water bath reaction at 40° C. for different times to determine a content of LNT2.

[0155] The results were shown shown in FIG. 4C: the optimal reaction time for the synthesis of LNT2 by HaHex74 was 2.5 h.

[0156] 4. Determination of Optimal Concentration of Lactose

[0157] The purified HaHex74 solution prepared in Example 2 was used as an enzyme solution to be tested, the reaction system was added with different concentrations of β-lactose, and was subjected to water bath reaction at 40° C. for 2.5 h to determine a content of LNT2.

[0158] The results were shown in FIG. 4D: the optimal lactose concentration for the synthesis of LNT2 by HaHex74 was 0.8 M.

[0159] 5. Determination of Optimal Enzyme Dosage

[0160] The purified HaHex74 solution prepared in Example 2 was used as an enzyme solution to be tested, the reaction system was added with different concentrations of HaHex74, and was subjected to water bath reaction at 40° C. for 2.5 h to determine a content of LNT2.

[0161] The results were shown in FIG. 4E: the optimal enzyme dosage for the synthesis of LNT2 by HaHex74 was 3-5 U.Math.mL.sup.−1.

EXAMPLE 4

Purification and Structural Identification of Synthesized LNT2

[0162] The product after the optimization of conditions in Example 3 was purified by HPLC. The collected product was subjected to TLC analysis for validation of purity (developing agent being n-butanol:ethanol:water=2:1:1 (v/v/v), and the color developer being methanol:sulfuric acid=95:5 (v/v)), and subjected to freeze drying to yield a white yellow powder sample.

[0163] The sample was dissolved in pure water and a high resolution primary mass spectrum of the sample was collected using a Thermo Scientific™ Q Exactive™ mass spectrometer in ESI ion source positive-ion mode.

[0164] The sample was dissolved in D.sub.2O and transferred to a nuclear magnetic dedicated tube, DSS (sodium 3-(trimethylsilyl)-1-propanesulfonate) was added as an internal standard, and the signal was collected at 298 K using a 500 MHz Varian V NMR SYSTEM™ instrument. All samples were analyzed for one-dimensional .sup.1H and .sup.13C spectra using an Agilent DD2 500 MHz NMR spectrometer, and for two-dimensional .sup.1H-.sup.13C heteronuclear single quantum coherence spectroscopy (HSQC) using standard pulse sequences and related parameters provided by Agilent.

[0165] The results showed that the purified product exhibited a single ion peak at a mass to charge ratio (m/z) [M+Na].sup.+ of 568.3 in the primary high-resolution mass spectrum (FIG. 5), indicating that the molecular weight of the product was 545.3, which was consistent with the molecular weight of LNT2 (545). Previous HPLC analysis of the HaHex74 synthesized product showed one major peak and one minor peak at the target product, which was shown to be an isomer by mass spectral analysis.

[0166] The structure of the transglycosylation product was further determined by NMR spectrum. .sup.1H chemical shift (FIG. 6) and .sup.13C chemical shift (FIG. 7) of the compound were obtained from the one-dimensional .sup.1H and .sup.13C spectra. The relationship between the carbon and hydrogen atoms of the compounds could be obtained from a two-dimensional heteronuclear single quantum coherence spectroscopy (HSQC) spectrum (FIG. 8). By data comparison, the HaHex74 product was consistent with the transglycosylation product of Bifidobacterium bifidum-derived β-N-acetylhexosaminidase mutant (Schmolzer et al. Glycosynthase principle transformed into biocatalytic process technology: Lacto-N-triose II production with engineered exo-hexosaminidase. ACS Catal. 2019, 9, 5503-5514), indicating that the compound is LNT2.

EXAMPLE 5

LNT2 Production

[0167] 1. Using Chitin as Starting Material

[0168] 120 g of ball-milled chitin powder (3%, w/v) (Jiang Zhengqiang et al., ZL 201811105536.4) was dissolved in 4 L of 20 mM citric acid buffer (pH 5.5), 5 U.Math.mL.sup.−1 of chitinase was added (Yang et al. Cloning, expression, purification and application of a novel chitinase from a thermophilic marine bacterium Paenibacillus barengoltzii. Food Chem., 2016, 192, 1041-1048), and hydrolyzed at 55° C. for 24 h.

[0169] 2. Preparation of LNT2

[0170] The hydrolysate (a mixture of chitobiose and N-acetylglucosamine) obtained in step 1 was centrifuged, the supernatant was collected and concentrated by 10 times, NaH.sub.2PO.sub.4 was added to adjust the pH to 7.5, 0.8 M of β-lactose and 4 U.Math.mL.sup.−1 HaHex74 (HaHex74 purified enzyme solution obtained in Example 2) were added, the mixture was reacted at 40° C., sampled at intervals, and content of LNT2 was determined by HPLC (see Example 3 for assay conditions).

LNT2 conversion ratio (%)=amount of synthesized LNT2 (M)/amount of chitobiose (M)×100%; wherein M represents the amount of a substance.

[0171] The results showed that the amount of LNT2 reached the maximum at 10 h with a conversion ratio of 13-20% and a concentration of 8-12 g.Math.L.sup.−1 (FIG. 9).

EXAMPLE 6

LNnT Production

[0172] The redundant β-lactose in the product of Example 5 described above was removed using an activated carbon column (75×1.5 cm), the product of Example 5 described above was subjected to gradient elution using a gradient mixer (TH-2000, Shanghai Qingpu Huxi Instrument Factory), and 0-25% (v/v) ethanol solution at a flow rate of 1 mL min.sup.−1, the eluent was collected and detected by TLC (see Example 4 for conditions) and HPLC (see Example 3 for conditions). The collected LNT2 solution was concentrated by 10 times with natural pH (pH 7.0) and 0.1 U mL.sup.−1 of β-galactosidase was added (Zeuner et al. Thermostable β-galactosidases for the synthesis of human milk oligosaccharides. New Biotechnol., 2016, 33, 355-360), the mixture was reacted at 50° C. and sampled at intervals, all samples were inactivated in a boiling water bath for 10 min and subjected to HPLC analysis (see Example 3 for conditions).

LNnT conversion ratio (%)=amount of synthesized LNnT (M)/amount of lactose (M)−100%; wherein M represents the amount of a substance.

[0173] The results showed that the collected LNT2 solution contained 20 mM β-lactose and 90 mM LNT2, and the reaction was conducted for 10 h, with the highest LNnT conversion ratio of 7-21%, and LNnT concentration of 1-3 g.Math.L.sup.−1 (FIG. 10).

[0174] The above are only preferred specific implementations of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily conceive modifications or replacements within the technical scope of the present invention, and these modifications or replacements shall fall within the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.

[0175] The content not described in detail in the specification belongs to the prior art known to those skilled in the art.

INDUSTRIAL APPLICATION

[0176] According to the invention, a glycoside hydrolase family 20 β-N-acetylhexosaminidase gene from Haloferula sp. (HaHex74) is efficiently expressed in P. pastoris. After high density fermentation in a 5-L fermentor, the enzyme activity of the fermentation broth can reach 3500 U.Math.mL.sup.−1, and the protein content is 9.7 g.Math.L.sup.−1, which is by far the highest expression level of β-N-acetylhexosaminidase. The newly invented β-N-acetylhexosaminidase HaHex74 displays excellent transglycosylation activity and can efficiently synthesize LNT2 (an important backbone precursor of human milk oligosaccharides) from natural donors chitobiose ((GIcNAc)2) and β-lactose, with a conversion ratio of 10-20%, which is by far the highest level for the synthesis of LNT2 by β-N-acetylhexosaminidase using the natural donor. Powdery chitin can be efficiently converted into LNT2 by chitinase and HaHex74, and LNnT can be efficiently produced by further cooperating with a β-galactosidase, with LNT2 and LNnT concentrations of 8-12 g.Math.L.sup.−1 and 1-3 g.Math.L.sup.−1, respectively. The HaHex74 disclosed by the invention possesses high-level expression, excellent hydrolysis properties and transglycosylation activity, which may make it potentially useful in the production of human milk oligosaccharides.