A method and a device for performing are qualitative and quantitative assay of biofilm-forming bacteria of at least one bacterial species contained in an aquatic system by use of a biochemical reaction of the bacteria with at least one substance which initiates a bacterium-specific metabolic reaction, as well as a colorimetric reagent which can be influenced by the metabolic reaction involving measurement of the spectrometric properties thereof forming the basis of the assay.

The presently disclosed fluidic sensor system and method comprise multifunctional nanoprobe-enabled capture for early detection of chemical and/or biological pathogens in a liquid sample. This sensor system and method can be used for food and environmental monitoring.

The present invention provides a method and probe for determining colibactin and a colibactin-producing bacterium. According to the present invention, there is provided a fluorescent probe for detecting myristoyl asparagine using, for example, a tissue sample and a fecal sample and detecting enzyme activity of ClbP.

The present invention relates to recombinant Gram-negative bacterial strains and the use thereof for delivery of heterologous proteins into eukaryotic cells.

A device (100) for detecting an analyte (132) includes a polymer material (112) over a substrate (110); a heat transfer element (114) thermally coupled to the substrate; a temperature modification device (118) thermally coupled to the heat transfer element; a controller (121) to produce a thermal (202) wave emanating from the heat transfer element; a flow cell (122) located and configured to pass a liquid (124) over the polymer material; a temperature sensor (134) to detect a temperature (T.sub.2) of the liquid passing over the polymer material; and a processor (123) to calculate a concentration of an analyte (132) in the liquid based at least in part on a phase shift between the thermal wave at the heat transfer element and an attenuated thermal wave (204) in the liquid. Related methods of forming such a device and detecting analytes are also disclosed.

The present disclosure provides an evaluation method of a pathogen inactivation effect, including: evaluating an inactivation effect with a maximum value of effective pathogen inactivation (MVEPI) as a standard reference index; where the MVEPI refers to a maximum of a log reduction factor after pathogen solutions of different concentrations before inactivation are treated according to a same inactivation method. The MVEPI can be obtained through the following steps: inactivating a sample containing pathogens with different initial concentrations through a pathogen inactivation method of tested pathogens; detecting changes in the concentration of pathogens with different initial concentrations before and after pathogen inactivation; and analyzing a relationship between the concentration of pathogens before inactivation and the concentration of pathogens after inactivation and the concentration before inactivation by linear fitting and other methods.

The disclosure relates to wireless detection of analytes (such as bacteria) labeled by electrically, dielectrically, or magnetically active nanoparticles, for example in quality control monitoring for food supply chain management. The disclosed apparatus includes a detection vessel or vial, a resonant sensor tag, an inductively coupled reader to induce and detect resonance in the sensor tag, and an active nanoparticle for labeling. The disclosed wireless detection apparatus and methods use active nanoparticles for the development of wireless sensor systems which can be designed to be compatible with the existing RFID technology infrastructure. For example, commercial RFID readers (e.g., hand-held) used for tracking and tracing operation can be used with the disclosed apparatus to induce and measure a corresponding resonance frequency.

Compositions for the treatment of colorectal cancer target bacterial biofilms in the gastrointestinal tract. Methods of treatment include one or more agents which target bacteria and the bacterial biofilms.

A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: ##STR00001## wherein the variables are described throughout the application.

The invention provides methods and kits for the rapid confirmation of an initial analyte test result. In a preferred embodiment, the process confirms the presence of a given microbial target in a mixed culture, or a mixed enrichment media, even when the competing organisms in the mix belong to related species, or are various biotypes of the same species.