The present technology is directed to the nucleic acid molecule encoding novel fluorescent proteins, in particular, green fluorescent proteins (GFPs), such as those that may be isolated from an organism of genus Rhacostoma, as well as compositions comprising the same and methods for analyzing a physiologically active substance in a cell wherein the fluorescent proteins are expressed in the cell.

The invention provides molecular tools to visualize, isolate, and manipulate the glial cells that are necessary for the formation, stability, and function of the synapse. The inventors identified a unique gene expression signature that distinguishes perisynaptic Schwann cells from all other Schwann cells. Using a combinatorial approach and coëxpressing two different fluorescence proteins, each using a different promoter, only those glial cells associated with the neuromuscular synapse are labeled.

The present disclosure relates to an assay for determining drug-target engagement in real-time. Specifically, the present disclosure provides a method for determining drug candidate-target interaction in real-time in living systems using Activity-based Reporter Gene Technology-Bioluminescence Resonance Energy Transfer (AbRGT-BRET) based assay. The said assay is useful for High Throughput Screening of compounds to a specific target.

Described herein are novel fluorescent sensors for Diacyl Glycerol (DAG) and phosphatidylinositol 4,5-bisphosphate (PIP2) that are based on circularly permuted fluorescent proteins. These sensors use less visible spectrum than FRET-based sensors, produce robust changes in fluorescence, and can be combined with one another, or with other sensors, in a multiplex assay on standard fluorescent plate readers or live cell imaging systems.

The invention provides molecular tools to visualize, isolate, and manipulate the glial cells that are necessary for the formation, stability, and function of the synapse. The inventors identified a unique gene expression signature that distinguishes perisynaptic Schwann cells from all other Schwann cells. Using a combinatorial approach and coxpressing two different fluorescence proteins, each using a different promoter, only those glial cells associated with the neuromuscular synapse are labeled.

The present disclosure relates to engineered protein metal ion sensors and methods of measuring metal ions. Disclosed herein are polypeptide metal ion sensors comprising engineered green-fluorescent polypeptides and engineered red-fluorescent polypeptides and methods of detecting metal ions. The polypeptide metal ion sensors disclosed herein can provide for ultrafast kinetics, larger absorption changes, and/or a greater fluorescence dynamic range.

The present disclosure provides systems and methods that may advantageously apply trained algorithms to automatically provide for coral growth, coral health, or coral resiliency, in-situ and ex-situ, for successful outplanting to a coral's native environment. In an aspect, the present disclosure provides an automated, computer-implemented method for growing resilient coral by obtaining one or more images of one or more corals and applying a machine learning-based classifier comprising a multi-class model on the one or more images to determine a resiliency of the one or more corals based at least on a plurality of coral health features and a plurality of coral environmental features.

The present invention is directed generally to eukaryotic cells comprising single-celled organisms that are introduced into the eukaryotic cell through human intervention and which transfer to daughter cells of the eukaryotic cell through at least five cell divisions, and methods of introducing such single-celled organisms into eukaryotic cells. The invention also provides methods of using such eukaryotic cells. The invention further provides single-celled organisms that introduce a phenotype to eukaryotic cells that is maintained in daughter cells. The invention additionally provides eukaryotic cells containing magnetotactic bacteria.

Described herein are novel fluorescent sensors for Diacyl Glycerol (DAG) and phosphatidylinositol 4,5-bisphosphate (PIP2) that are based on circularly permuted fluorescent proteins. These sensors use less visible spectrum than FRET-based sensors, produce robust changes in fluorescence, and can be combined with one another, or with other sensors, in a multiplex assay on standard fluorescent plate readers or live cell imaging systems.

Methods for extending light-emitting time of a solution of a calcium-binding photoprotein that instantaneously emits light by binding to calcium ions are provided. In the light-emitting reaction system of a solution of a calcium-binding photoprotein, a light-emitting reaction is performed in the presence of an anion capable of binding to the calcium ion or the cation that can be substituted for the calcium ion and/or a cation that can bind to the calcium-binding site of the calcium-binding photoprotein with a lower affinity than the calcium ion or the cation that can be substituted for the calcium ion without activating the calcium-binding photoprotein.