A porous transparent electrode is formed where a film comprising of semiconducting nanoparticles is decorated with polyoxometalates (POMs) bonded to their surfaces. The semiconducting nanoparticles are transparent metal oxide. The semiconducting nanoparticles include tin-doped indium oxide (ITO), fluorine-doped tin oxide (FTO), or titanium dioxide (TiO.sub.2). In an embodiment, the POM is [SiW.sub.12O.sub.40].sup.4−; [α-P.sub.2W.sub.18O.sub.62].sup.6−; or [α.sub.2-P.sub.2W.sub.17O.sub.61].sup.10−. The semiconducting nanoparticles bond to the POM through a combination of electrostatic interactions and hydrogen bonds. The porous transparent electrode can be placed in a protonated form or ion-paired with alkali metal cations or tetraalkylammonium cations.

This invention provides a sensing electrode for detecting at least one target gas in a gas mixture having at least one interference gas. In one embodiment, the sensing electrode has: (a) a layer of sensing nanoparticles; (b) a reaction interface; and (c) a solid state electrolyte; each of the sensing nanoparticles has a catalytic core and a photoactive porous shell, the catalytic core breaks down said at least one interference gas, the photoactive porous shell enhances electrochemical reaction at said reaction interface when illuminated with light of a specific wavelength.

The present disclosure belongs to the technical field of biosensors and particularly provides a construction method for a photocathode indirect competition sensor and an evaluation method. The construction method includes: using Z-type Bi.sub.2O.sub.3/CuBi.sub.2O.sub.4 as a sensing platform; calculating a photoinduced electron Z-type transfer path and an energy band structure of Bi.sub.2O.sub.3 and CuBi.sub.2O.sub.4 using a density functional theory (DFT); and constructing a Bi.sub.2O.sub.3/CuBi.sub.2O.sub.4-based biosensor. A photoelectrochemical (PEC) photocathode biosensor based on a Bi.sub.2O.sub.3/CuBi.sub.2O.sub.4 heterojunction prepared through the solution has good repeatability, reproducibility, stability, and specificity for detecting a target. The PEC biosensor constructed in the solution of the present disclosure has a broad application prospect in the fields of healthcare, environment, and food.

This disclosure relates to a biosensor, and methods of use thereof, for detecting a target in a sample comprising a photoelectrode comprising a conductive substrate and a photoactive material; a population of capture probes functionalized on the photoelectrode wherein the capture probes are capable of binding to the target and a reporter moiety; and the reporter moiety comprising a detectable label and a capture probe binding portion; wherein exposure of the target to the population of the capture probes results in binding of the target to a fraction of the population which results in a decrease in detection signal intensity compared to the intensity in the absence of the target, and subsequent binding of the reporter moiety to the remaining unbound capture probes results in an increase in detection signal intensity that is less than an increase from the reporter moiety binding to capture probes not exposed to the target.

This disclosure provides systems, methods, and apparatus related to electrochemistry. In one aspect, an apparatus includes a light source and a potentiostat. The potentiostat is operable to be connected to an electrochemical cell. A counter electrode connection of the potentiostat is operable to be connected to a photoelectrode of the electrochemical cell. A working electrode connection of the potentiostat is operable to be connected to the working electrode of the electrochemical cell. The light source positioned to illuminate the photoelectrode when the light source is operating. When the apparatus is in operation, the potentiostat is used to bias the photoelectrode to allow for control of the voltage applied to the electrochemical cell, and an intensity of light incident upon the photoelectrode is varied to allow for control of the current applied to the electrochemical cell.

This disclosure relates to a biosensor for detecting a target analyte in a sample comprising a first and second photoelectrode each comprising conductive substrate and photoactive material, a first and second capture probe functionalized on the first and second photoelectrode, respectively, and optionally one or more reporter moieties comprising a detectable label, wherein the first and second capture probe each, independently, provides a distance between the detectable label and the photoactive material in the presence of the target analyte, wherein intensity of detection signal dictated by the distance is generated from the first and second photoelectrode by transfer of electrons between the detectable label and the photoactive material, wherein a higher, or higher increase than in absence of sample, in the intensity of the detection signal from the first as compared to the second photoelectrode in the presence of the sample, is indicative of the presence of the target analyte.

A biocompatible electrochemical flow cell (eFC) for high resolution imaging of anode and cathode biofilms using laser scanning confocal microscopy employs optically transparent indium tin oxide (ITO)-coated electrode configured to allow observation of the flow chamber. This enables correlation of electrochemical signatures with biofilm development in real-time.

Provided is a microelectrode having a layered structure, including a layer containing a polymer compound having an aromatic ring (polymer compound layer) and a layer containing a conductive material (conductive layer), wherein a thickness of the polymer compound layer is 10 to 900 nm, a thickness of the conductive layer is 0.3 to 10 nm, and the microelectrode has a three-dimensional curved shape.

Immunosensors according to present embodiments combine a sandwich bioassay with an electrochemical surface plasmon resonance device for electrochemical detection of analytes from a sample, whereby a coated substrate for receiving an electroactive probe may be located in a flow cell, and the coated substrate comprises a first layer which is a silver (Ag) layer and a second layer which is a gold (Au) layer arranged so that the gold layer isolates the silver layer from an operating environment.

A porous transparent electrode is formed where a film comprising of semiconducting nanoparticles is decorated with polyoxometalates (POMs) bonded to their surfaces. The semiconducting nanoparticles are transparent metal oxide. The semiconducting nanoparticles include tin-doped indium oxide (ITO), fluorine-doped tin oxide (FTO), or titanium dioxide (TiO.sub.2). In an embodiment, the POM is [SiW.sub.12O.sub.40].sup.4−; [α-P.sub.2W.sub.18O.sub.62].sup.6−; or [α.sub.2-P.sub.2W.sub.17O.sub.61].sup.10−. The semiconducting nanoparticles bond to the POM through a combination of electrostatic interactions and hydrogen bonds. The porous transparent electrode can be placed in a protonated form or ion-paired with alkali metal cations or tetraalkylammonium cations.