The invention is a multiplex, pulsed-field capillary electrophoresis instrument with the ability to analyze DNA fragments with sizes greater than 150,000 base pairs. The parallel capillary electrophoresis system allows for the simultaneous analysis of at least 12 samples while applying a pulse or varying electric field for separation. Sequences of pulse-field electric fields are iterated to achieve accurate separation of DNA smears.

Techniques described herein can apply AC signals with different phases to different groups of nanopore cells in a nanopore sensor chip. When a first group of nanopore cells is in a dark period and is not sampled or minimally sampled by an analog-to-digital converter (ADC) to capture useful data, a second group of nanopore cells is in a bright period during which output signals from the second group of nanopore cells are sampled by the analog-to-digital converter. The reference level setting of the ADC is dynamically changed based on the applied AC signals to fully utilize the dynamic range of the ADC.

A system for molecular mapping includes a semiconductor substrate defining a reservoir to receive a sample of molecules and a nanofluidic channel in fluid communication with the reservoir. The system also includes a plurality of electrodes, in electrical communication with the nanofluidic channel, to electrophoretically trap the sample of molecules in the nanofluidic channel. At least one avalanche photodiode is fabricated in the semiconductor substrate and disposed within an optical near-field of the nanofluidic channel to detect fluorescence emission from at least one molecule in the sample of molecules.

An electrochemical microarray chip to detect specific sequences of single-stranded DNA (ssDNA) or single-stranded RNA (ssRNA) target molecules in solution using a microarray of microspots of probe molecules immobilized on an electrode. The chip pertains to both regulating the immunospecific binding to the array of probes on the electrode and their subsequent detection on the microarray spots on the monolith electrode by electrochemical methods. The device can quantitatively measure the concentration of target molecules of specific sequence at high specificity and high sensitivity.

A pore forming method in which a pore is formed in such a way that a first voltage is applied between electrodes that are disposed with a film in an electrolytic solution therebetween; a second voltage, which is lower than the first voltage, is applied between the electrodes; a current that flows between the electrodes owing to the application of the second voltage is measured; it is judged whether a value of a current is equal to or larger than a predefined threshold; and if the value of the current is smaller than the threshold, the above sequence is repeated until a pore is formed. In this case, the second voltage is a voltage that makes the value (I.sub.PF) of the current flowing through the film practically 0. With the use of the above method, a nanopore is formed in the film simply, easily, and accurately.

A wireless circuit including an electrode array with a nanoscale dielectric disposed between two electrodes allows for wirelessly powered manipulation of particles in a liquid solution, air, or gaseous media via dielectrophoretic forces. The electrode array includes a first electrode, a second electrode, and a nanoscale dielectric layer between the first and second electrode. An inductive coupler is operatively coupled to the electrode array and configured to receive wireless power or wireless signals.

The present invention provides an integrated type microfluidic electrochemical biosensor system for rapid biochemical analysis and the usage of the system. The system comprising: a continuous feeding unit for sequentially conveying lead eluent, sample solution, sample eluent, signal probe solution, signal probe eluent and electrochemical detection buffer solution; a microfluidic chip consists of one or more micro-channel network, the microfluidic chip covers the electrode array to form a channel system, capture probes which have interaction with the said sample solution fixed on the surface of the electrode array, said channel system is connected with the continuous feed unit; and a power system for providing power to said continuous feeding unit. The invention innovatively combine three technologies of planar electrode arrays, microfluidic chip technology and continuous feeding unit together, and the integrated type microfluidic electrochemical biosensing system which is small in size and low in cost and has a wide application prospect is provided.

There is provided a method for distributing power in an electrophoresis system from a power supply to each channel of an interfaced cassette connected to a pedestal located on the electrophoresis system, the method comprising: receiving a power signal from the power supply at the pedestal; receiving at least one externally generated control signal; and, modulating the power signal at a processor electrically coupled to the pedestal in dependence upon the control signal to generate a modulated power signal defined for each said cassette channel of the pedestal.

The present disclosure generally relates to devices for effecting epitachophoresis. Epitachophoresis may be used to effect sample analysis, such as by selective separation, detection, extraction, and/or pre-concentration of target analytes such as, for example, DNA, RNA, and/or other biological molecules. Said target analytes may be collected following epitachophoresis and used for desired downstream applications and further analysis.

A particle manipulation device includes a substrate and a microchannel included in the substrate and configured to receive a fluid including particles therein. A biasing structure is formed on the substrate adjacent to, but outside the microchannel. The biasing structure is configured to dispense radiation at a frequency to bias movement of the particles within the microchannel from outside the microchannel.