An electrophoresis method, system, and gel recover biological substances from the gel with high efficiency. The method uses an electrophoresis gel having an injection hole into which biological substances are injected and a recovery hole from which the biological substances are recovered. The electrophoresis method includes injecting the biological substances into the injection hole, and applying an electric field penetrating the injection and recovery holes. A vertical axis in a downward direction as a positive direction is set as an X-axis, an axis which is perpendicular to the X-axis is set as a Y-axis, and coordinates of a bottom of the recovery hole are set as (X.sub.C, Y.sub.C). The X coordinate X.sub.C of the bottom of the recovery hole satisfies X.sub.C>X.sub.1 when the biological substance is electrophoresed to coordinates (X.sub.1, Y.sub.C) in the recovery hole from a bottom of the injection hole in the applying the electric field.

A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.

A high-throughput multiplexing electrophoretic gel system is disclosed. The system includes a gel casting device which includes an interior, gel casting chamber by which a polymerized gel layer is formed. The polymerized gel layer includes a plurality of integrally formed sample loading wells. The wells are aligned to be simultaneously loaded with samples via an automated microliter multi-pipette sample loader. The sample-loaded gel layer is adapted to undergo immersed horizontal electrophoresis separation.

Methods, systems and devices that allow independently applied pressures to a BGE reservoir and a sample reservoir for pressure-driven injection that can inject a discrete sample plug into a separation channel that does not require voltage applied to the sample reservoir and can allow for in-channel focusing methods to be used. The methods, systems and devices are particularly suitable for use with a mass spectrometer.

An electrophoresis system includes a plurality of capillaries in which electrophoresis of a sample is performed, a light source that irradiates detection positions of the capillaries with light, a light detector that detects light generated by the irradiation with the light by the light source that depends on components contained in the sample, and a buffer storage unit into which one end of the plurality of capillaries are inserted at a time of the electrophoresis of the sample and in which a buffer is stored. A computer controls an electrophoresis condition of each of the plurality of capillaries such that arrival times for the components that move in the plurality of capillaries to reach the detection positions are shifted from each other. As a result, it is possible to detect fluorescence signals at different timings in each of the plurality of capillaries.

In a method for producing transparent biological preparations for examination by light microscopy biological tissue is electrophoretically clarified in that the tissue is immersed in an aqueous alkaline electrophoresis solution and is exposed to an electric field in the electrophoresis solution. The electrophoresis solution contains a buffer base, the cations of which have a molecular weight of at least 50 Da, at a concentration of 5 to 100 mol/m.sup.3 and a non-ionic detergent at a concentration of 0.1 to 10% (w/v).

A microfluidic chip device for the purification of radiochemical compounds includes a chip having an injection channel and intersecting branch channels with a plurality of valves are located along the injection channel and branch channels and configured to retain a plug of solution containing the radiochemical compound. The chip further includes a serpentine channel segment (for separation) coupled to the output of the injection channel. A high voltage power source advances the plug of solution through the purification region and into the downstream fraction collection channel. The chip includes a downstream fraction collection channel coupled to the serpentine channel segment and having an optical and radiation detection regions. One or more branch fraction channels intersect with the fraction collection channel and include valves located therein so that the radiochemical compound that is detected using a radiation detector is directed into the desired branch fraction channel for subsequent use.

In one general aspect, an electrophoretic measurement method is disclosed that includes providing a vessel that holds a dispersant, providing a first electrode immersed in the dispersant, and providing a second electrode immersed in the dispersant. A sample is placed at a location within the dispersant between the first and second electrodes with the sample being separated from the electrodes, an alternating electric field is applied across the electrodes, and the sample is illuminated with temporally coherent light. A frequency shift is detected in light from the step of illuminating that has interacted with the sample during the step of applying an alternating electric field, and a property of the sample is derived based on results of the step of detecting.

The invention relates to an electrophoresis device (1) for use in a method for producing transparent biological samples (2), comprising a reaction frame (3), the reaction frame (3) having an open top side (4) and a bottom side (5) opposite the top side (4), characterized in that the bottom side (5) has at least partially one opening (6). The invention also relates to a use of a sample cassette (19) for an electrophoresis method.

The invention is an improved ultraviolet absorption-based multiplex capillary electrophoresis instrument with at least four and preferably six user-accessible vertically stacked drawers. An x-z stage moves samples from the user accessible drawers to the capillary array for analysis. A computer program allows users to add capillary electrophoresis jobs to a queue corresponding to the analysis of rows or plates of samples without stopping or interrupting runs in progress. A double-set of enclosures surrounding the light source enables collection of high-quality data. The invention also may include a capillary reservoir with separate flow channels for the capillary tips and electrode.