A dual dielectropheretic article for monitoring cell migration includes: a membrane to selectively migrate a plurality of cells across the membrane, the membrane including: a first surface to receive the cells; a second surface opposed to the first surface; and a plurality of communication paths disposed in the membrane to provide the selective migration of the cells across the membrane from the first surface to the second surface; a first electrode disposed on the first surface to: provide an electric field for dielectrophoresis of the cells at the first surface; and provide a first potential for monitoring an impedance at the first surface; and a third electrode disposed on the second surface to: provide an electric field for dielectrophoresis of the cells at the second surface; and provide a third potential for monitoring an impedance at the second surface.

According to one embodiment, an analyzing apparatus is disclosed. The analyzing apparatus includes a generation device to generate an electromagnetic force for drawing a particle in a first liquid that is to be supplied onto a substrate, wherein the electromagnetic force draws the particle to the substrate side, and a measurement device to measure an image the first liquid on the substrate. The analyzing apparatus further includes a control device to control the generation device, and a liquid channel structure that includes a first supply channel for supplying a liquid onto the substrate and a first drain channel for draining a liquid on the substrate.

A microfluidic transport system for transporting microdroplets in three spatial dimensions among layers of a layered microfluidic system. In an example arrangement, a first microfluidic layer for transporting microdroplets in two spatial dimensions responsive to electric fields created by electrical operation of electrodes is fluidically connected by one or more conduits to other microfluidic layers. Microdroplets can be transported through the one or more conduits so as to be moved among a plurality of layered microfluidic arrangements. The resulting layered system can be used for heat transfer, fluidic transfer, and other uses, and can be implemented using materials such as metal, glass, polymer, plastic, layered materials, fibrous materials, etc. In some applications the layered system can be implemented within a printed circuit board, integrated circuit housing. Example applications include integrated circuit cooling, energy harvesting, microfluidic processing systems, chemical reactors, biochemical reactors, chemical analysis arrangements, biochemical analysis arrangements, and other apparatus.

A separation unit is a configuration that is arranged vertically, and thus bubbles generating from the electrode will not negatively influence the contact location between the transfer membrane and separation unit. An anode (32) is arranged at a position separated by a certain distance in the conveying direction (X) of the transfer membrane (1) from the dispensing part (50a) of an electrophoresis gel chip (50). An insulating electrode cover (35) for setting free bubbles generating from the anode (32) is arranged at an upper part of the anode (32).

Devices and methods for characterization of analyte mixtures are provided. Some methods described herein include performing enrichment steps on a device before expelling enriched analyte fractions from the device for subsequent analysis. Also included are devices for performing these enrichment steps.

Method, apparatus, and computer program product for a microfluidic channel having a cover opposite its bottom and having electrodes with patterned two-dimensional conducting materials, such as graphene sheets integrated into the top of its bottom. Using the two-dimensional conducting materials, once a fluid sample is applied into the channel, highly localized modulated electric field distributions are generated inside the channel and the fluid sample. This generated field causes the inducing of dielectrophoretic (DEP) forces. These DEP forces are the same or greater than DEP forces that would result using metallic electrodes because of the sharp edges enabled by the two-dimension geometry of the two-dimensional conducting materials. Because of the induced forces, micro/nano-particles in the fluid sample are separated into particles that respond to a negative DEP force and particles that respond to a positive DEP. Microfluidic chips with microfluidic channels can be made using standard semiconductor manufacturing technology.

Devices and methods for characterization of samples are provided. Samples may comprise one or more analytes. Some methods described herein include performing enrichment steps on a device. Some methods described herein include performing mobilization of analytes. Analytes may then be further processed and characterized.

Microfluidic chips that can comprise thin substrates and/or a high density of vias are described herein. An apparatus comprises: a silicon device layer comprising a plurality of vias, the plurality of vias comprising greater than or equal to about 100 vias per square centimeter of a surface of the silicon device layer and less than or equal to about 100,000 vias per square centimeter of the surface of the silicon device layer, and the plurality of vias extending through the silicon device layer; and a sealing layer bonded to the silicon device layer, wherein the sealing layer has greater rigidity than the silicon device layer. In some embodiments, the silicon device layer has a thickness between about 7 micrometers and about 500 micrometers while a via of the plurality of vias has a diameter between about 5 micrometers and about 5 millimeters.

A sorting device is provided. The sorting device includes: a carrier substrate; an input unit disposed on the carrier substrate for inputting a biological sample into the sorting device; a porous material disposed on the carrier substrate and adjacent to the input unit, wherein the porous material contains antigen molecules having specificity to a target biological analyte; a driving module generating at least one driving force in the porous material so as to sort the biological sample based on the affinity for the antigen and the driving force; and an output unit disposed on the carrier substrate and adjacent to the porous material for collecting the sorted target biological analyte. A sorting method is also provided.

The present invention relates to a method for identifying a target protein using a thermal stability shift-based fluorescence difference in two-dimensional gel electrophoresis, and more specifically, a method for identifying a protein, which is a target of a specific drug, by analyzing, by means of a fluorescence difference in two-dimensional gel electrophoresis, a thermal stability shift in the protein when a specific drug, preferably a bioactive molecule, binds to the target protein.