A method of detecting particles (1), e.g. proteins, after separation of particles based on their specific features, e.g. charge, size, shape, density, as series of single light scattering events created by the individual particles is described. The particles (1) are separated from each other along the separation path (11) and particles have specific arrival times at the target side depending on the particle features. The detecting step comprises an interferometric sensing of the light scattered at individual particles bound or transient in the detection volume (30). Parameters of the scattering light signals e.g. the interferometric contrast are analysed for obtaining specific particle features, e.g. size, mass, shape, charge, or affinity of the particles (1). Furthermore, a detection apparatus (100) being configured for detecting particles (1) is described.

A method of separating and analysing a plurality of components in a heterogeneous sample is provided. The method comprising the steps of: introducing a separation fluid into a separation channel that is elongate in a first direction; introducing the heterogeneous sample into said channel; separating, in the first direction, the components in the sample; introducing an auxiliary fluid into said channel; creating a lateral distribution of the components in a second direction substantially perpendicular to the first direction; and determining, sequentially, a property of each of the components based on the regimen by which the lateral distribution was created. An apparatus for separating and analysing a plurality of components in a heterogeneous sample is also provided.

A system includes a housing, a cartridge retainer disposed within the housing, a detection assembly disposed within the housing, and a reagent tray holder movably disposed in the housing. The cartridge retainer configured to receive a capillary cartridge having a capillary. The detection assembly includes at least one emitter, a first detector, and a second detector. The detection assembly is configured to transition between a first configuration, in which the first detector detects a first output of the at least one emitter, and a second configuration, in which the second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of the capillary cartridge in fluid communication with a reagent volume.

A method of quantifying charge variants within an analyte may include introducing a sample buffer comprising the analyte into a capillary, separating charge variants within the sample buffer along an isoelectric gradient, incubating the capillary in a detection antibody, quantifying a relative abundance of a charge variant based on a signal that corresponds to the detection antibody. The method may further include generating an electropherogram, wherein the electropherogram includes a plot of a strength of a signal generated by a reporter molecule versus an isoelectric point along the isoelectric gradient where the signal was detected.

Methods, devices, and systems for performing isoelectric focusing reactions are described. The systems or devices disclosed herein may comprise fixtures that have a membrane. In some instances, the disclosed devices may be designed to perform isoelectric focusing or other separation reactions followed by further characterization of the separated analytes using mass spectrometry. The disclosed methods, devices, and systems provide for fast, accurate separation and characterization of protein analyte mixtures or other biological molecules by isoelectric point.

Methods for detecting and/or discriminating between variants of an antibody contaminating protein or multiple antibodies in a sample by a physical parameter, in which the method includes: separating protein components of a sample by molecular weight or charge in one or more capillaries using capillary electrophoresis; immobilizing the protein components of the sample within the one or more capillaries; contacting the protein components within the one or more capillaries with one or more primary antibodies that specifically bind to the antibody, the contaminating protein or multiple antibodies in the sample, thereby detecting and/or discriminating between variants in the sample.

The presently claimed and described technology is directed to an acidic high polymer composition comprising about 1.0% (w/v) polymer and about 4% (v/v) carboxylic acid. The acid high polymer composition may be used as a neutral capillary storage or conditioning solution or in a method of improving capillary isoelectric focusing (cIEF) robustness or performance. The technology is also directed to a kit comprising an acidic high polymer composition, at least one stabilizer, an anolyte, and a catholyte.

Described herein is a bias-free high-throughput and high-yield continuous isoelectric fractionation (CIF) nanocarrier fractionation technique based on distinct isoelectric points. The nanocarrier fractionation platform is enabled by a robust and tunable linear pH profile provided by water-splitting at a bipolar membrane and stabilized by flow without ampholytes.

The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.

The present disclosure generally relates to devices and methods for effecting epitachophoresis. Epitachophoresis may be used to effect sample analysis, such as by selective separation, detection, extraction, and/or pre-concentration of target analytes such as, for example, DNA, RNA, and/or other biological molecules. Said target analytes may be collected following epitachophoresis and used for desired downstream applications and further analysis.