Methods and system for identification of dimer species using online chromatography and electrospray ionization mass spectrometry are provided. Also provided are methods and system for quantitation of heterodimer species using immunoprecipitation and liquid chromatography-mass spectrometry.

A method for detecting a trace protein in a sample system, comprising: providing a primary antibody-modified immunomagnetic bead and a secondary antibody-modified nanoparticle for a to-be-detected protein; mixing and incubating the primary antibody-modified immunomagnetic bead and the secondary antibody-modified nanoparticle with a sample system containing the to-be-detected protein, so as to form a complex having a double-antibody sandwich structure; and allowing the complex to pass through a micro-nano pore device in the form of a single particle and trigger an electrical pulse signal, analyzing the electrical pulse signal to obtain a charge state or a volume/mass state of the complex, and calculating, according to the charge state or the volume/mass state, the amount of the to-be-detected protein in the sample system.

Disclosed is a device including a substrate having a plurality of detection areas to receive at least a portion of a sample having an analyte or suspected of having an analyte and at least one reference marker adjacent to at least one of the detection areas, wherein each of the detection areas detects a specific analyte and the at least one reference marker defines a scale mark, a shape mark, a color mark, or a combination thereof. Also disclosed is an imaging system and method for using the device and imaging system.

Disclosed are methods and systems for detecting SARS-CoV-2 analytes in dried samples, as for example, dried blood spots. For example, disclosed is a method for measuring an analyte of interest in a dried sample comprising: (a) obtaining a dried sample from a subject; (b) extracting the analyte of interest from the dried sample; and (c) detecting the analyte of interest extracted from the dried sample. In certain embodiments, the analyte of interest is an analyte specific to SARS-CoV-2. Also, the method may include a step of determining a cutoff index (COI) indicative of whether the subject has a detectable amount of the analyte of interest and so is defined as positive, or does not contain a detected amount of the analyte of interest and so is defined as negative, or is defined as indeterminate.

The present invention relates generally to an assay for detecting and differentiating single or multiple analytes, if present, in a fluid sample, including devices and methods of use of the same.

A method, device, computer program and related immunoassay are disclosed for assessing the efficacy of a statin selected from, for example, selected from RvT1 (7,13,20-trihydroxy-8,10,14,16Z,18-docosapentaenoic acid), RvT2 (7,12,13-trihydroxy-8,10,14,16Z,19Z-docosapentaenoic acid), RvT3 (7,8,13-trihydroxy-9,11,14,16Z,19Z-docosapentaenoic acid) and RvT4 (7,13-dihydroxy-8,10,14,16Z,19Z-docosapentaenoic acid), for use in the treatment of an inflammatory condition in an individual patient, which comprises measuring the levels of at least one 13-series resolvin in biological samples obtained from the patient before and after administration of the statin, wherein an increase in the level of the resolvin after administration of the statin is indicative of efficacy of the statin. Also disclosed is a method of storing a biological sample to preserve lipid mediators in the sample comprising placing the sample in an organic solvent and storing the sample at a temperature of ≤−75° C.

A microfluidic device for and methods of using surface-attached posts and capture beads in a microfluidic chamber is disclosed. For example, the microfluidics device includes a pair of substrates separated by a gap and thereby forming a reaction (or assay) chamber therebetween. A field of actuatable surface-attached posts (e.g., magnetically responsive microposts) is provided on one or both of the substrates. The surface-attached posts are functionalized with capture beads. Additionally, methods are provided of functionalizing the surface-attached posts with the capture beads. Additionally, methods are provided of using the surface-attached posts that are functionalized with capture beads in a microfluidics device for binding a target of interest. Further, a bead spraying system and method is provided for spraying magnetically responsive and/or non-magnetically responsive beads atop and/or among a field of surface-attached microposts for use in a microfluidic device.

A blood biomarker analysis systems providing fast biomarker identification includes a multimodal bioassay device having a biosensor within a portable pipette-shaped device and using nanoplasmonic barcode detectors, such as formed of antibody conjugated gold nanoparticle arrays (AuNPs), capable of capturing any of a plurality of biomarkers. The biomarker analysis system further includes the pipette-shaped device being smartphone-connected and portable to form a highly accurate, point-of-care bioassay device.

A biochip is capable of highly sensitively detecting a measurement target substance. The biochip includes a selective-binding substance capable of selectively binding to the measurement target substance, the selective-binding substance being immobilized on a surface of a substrate through a polymer containing a unit represented by Formula (Ia) or (Ib):

##STR00001##

wherein, R.sup.1 represents C.sub.1-C.sub.4 alkylene; R.sup.2 represents R.sup.3, OR.sup.4, or NHR.sup.5; R.sup.3 and R.sup.5 each independently represent a hydrogen atom or C.sub.1-C.sub.4 alkyl; R.sup.4 represents C.sub.1-C.sub.4 alkyl; R.sup.6 represents C.sub.1-C.sub.2 alkylene; and R.sup.7 and R.sup.8 each independently represent a hydrogen atom or C.sub.1-C.sub.4 alkyl.

A system (1) for detecting an analyte of interest in a sample is disclosed that comprises a measurement chamber (21) for metering the sample and including a defined concentration of an activator (27) causing the generation of a product when interacting with the analyte of interest, a heating element (31) thermally coupled to the measurement chamber, a controller (33) adapted to control the heating element such that the measurement chamber is maintained at a defined temperature (T.sub.d), a sensor (35) adapted to detect said product, a timer (37) adapted to time an interaction time between the sample and the activator; and a processor (39) responsive to the sensor and the timer. The processor is adapted to, upon addition of the sample to the measurement chamber, determine an amount of the analyte of interest in the sample from a sensor signal indicative of an amount of said product in the measurement chamber provided by the sensor prior to termination of said interaction; known interaction kinetics between the analyte of interest and the activator at the defined temperature and the defined concentration; and the interaction time at time of generation of the sensor signal. A method of detecting an analyte of interest in a sample using such a system is also disclosed.