Provided herein are macromolecular structures comprising charged macromolecules. In particular, provided herein are synthetic neutrophil extracellular traps and uses thereof.

A complex having the structure of formula (I)

##STR00001##

is disclosed. L.sub.1, L.sub.2, L.sub.3, L.sub.4, and L.sub.5 may be or include independently metal-coordinating ligands selected from the group consisting of neutral ligands, anionic ligands, and mixed ligands, and combinations thereof. In a non-limiting embodiment, the complex is an N-heterocyclic carbene (NHC) Re(I) complex having an unsubstituted or substituted benzimidazol-2-ylidene ligand. The complex may be included in a pharmaceutical composition for treating gram (+) bacterial infections.

A complex having the structure of formula (I)

##STR00001##

is disclosed. L.sub.1, L.sub.2, L.sub.3, L.sub.4, and L.sub.5 may be or include independently metal-coordinating ligands selected from the group consisting of neutral ligands, anionic ligands, and mixed ligands, and combinations thereof. In a non-limiting embodiment, the complex is an N-heterocyclic carbene (NHC) Re(I) complex having an unsubstituted or substituted benzimidazol-2-ylidene ligand. The complex may be included in a pharmaceutical composition for treating gram (+) bacterial infections.

Provided are a near-infrared II polymer fluorescent microsphere and a method for preparing the same. The method includes steps of 1) dissolving fluorochrome in a water-immiscible organic solvent, thus obtaining a fluorochrome solution; 2) distributing a polymer microsphere into a sodium dodecyl sulfonate solution, thus obtaining a microsphere solution with the polymer microsphere as a carrier for the fluorochrome; 3) subjecting a first mixture of the fluorochrome solution and the microsphere solution to ultrasonic treatment, thus obtaining an emulsion; 4) swelling the emulsion such that the fluorochrome solution enters nanopores formed during swelling of the polymer microsphere, thus obtaining a second mixture; and 5) heating the second mixture to volatilize the organic solvent, such that the fluorochrome is crystallized out and encapsulated in the nanopores, thus obtaining the near-infrared II polymer fluorescent microsphere.

The present disclosure relates to a sensor for the detection of analytes, in particular for the detection of biomolecules. The sensor includes a (bio)compatible sensing layer including a polymer matrix or gel matrix, particularly a polymer gel matrix, organic nanoparticles and, optionally, one or several cell adhesion layer(s). The cell adhesion layer(s) can be varied depending on the type of cells. In the presence of the analytes, the organic nanoparticles are capable of photon up-conversion emission. The sensor further optionally includes plasmonic metal nanoparticles. The present disclosure further relates to methods of producing such a sensor and to uses of such a sensor.

The present disclosure relates to a sensor for the detection of analytes, in particular for the detection of biomolecules. The sensor includes a (bio)compatible sensing layer including a polymer matrix or gel matrix, particularly a polymer gel matrix, organic nanoparticles and, optionally, one or several cell adhesion layer(s). The cell adhesion layer(s) can be varied depending on the type of cells. In the presence of the analytes, the organic nanoparticles are capable of photon up-conversion emission. The sensor further optionally includes plasmonic metal nanoparticles. The present disclosure further relates to methods of producing such a sensor and to uses of such a sensor.

Provided are a test strip for short-wave near infrared immunofluorescence chromatographic detection, a system for immunofluorescence chromatographic detection and a method for quantifying an analyte in a sample. The test strip for short-wave near infrared immunofluorescence chromatographic detection includes: a body, defining a sample region, a binding region, a detecting region and an adsorbing region connected with one another sequentially; a first antibody, labeled with a fluorescent microsphere, coated on the binding region and configured to specifically recognize an analyte; a detecting line and a quality control line, located in the detecting region, wherein the detecting line closes to the binding region; a second antibody, coated on the detecting line and configured to specifically recognize the analyte; and a third antibody, coated on the quality control line and configured to specifically recognize the first antibody, in which the fluorescent microsphere is a near-infrared II polymer fluorescent microsphere.

Provided are a test strip for short-wave near infrared immunofluorescence chromatographic detection, a system for immunofluorescence chromatographic detection and a method for quantifying an analyte in a sample. The test strip for short-wave near infrared immunofluorescence chromatographic detection includes: a body, defining a sample region, a binding region, a detecting region and an adsorbing region connected with one another sequentially; a first antibody, labeled with a fluorescent microsphere, coated on the binding region and configured to specifically recognize an analyte; a detecting line and a quality control line, located in the detecting region, wherein the detecting line closes to the binding region; a second antibody, coated on the detecting line and configured to specifically recognize the analyte; and a third antibody, coated on the quality control line and configured to specifically recognize the first antibody, in which the fluorescent microsphere is a near-infrared II polymer fluorescent microsphere.

The present invention provides a membrane carrier 3 comprising a flow path 2 and a detection zone 3y, wherein a microstructure is provided at the bottom of the flow path 2 and a mean surface roughness of the microstructure is 0.005 to 10.0 m.

The present invention provides a membrane carrier 3 comprising a flow path 2 and a detection zone 3y, wherein a microstructure is provided at the bottom of the flow path 2 and a mean surface roughness of the microstructure is 0.005 to 10.0 m.