The present invention provides a biosensor for detecting allergies. The biosensor comprises a substrate; an electrode layer formed on a portion of an upper side of the substrate; a single-walled carbon nanotube layer formed on another portion of the upper side of the substrate; a linker layer formed on the single-walled carbon nanotube layer; and an antibody which is immobilized and bonded to the linker layer. In the biosensor for detecting allergies according to the present invention, 1-pyrenebutanoic acid succinimidyl ester is comprised as a linker on the substrate which is coated with a single-walled carbon nanotube, and the antibody which is capable of capturing an allergy-inducing protein is immobilized on the linker. When the allergy-inducing protein is captured by the antibody, a resistance value is changed, so that the allergy-inducing protein is capable of being detected with high sensitivity in a short time by sensing the changed resistance value.

The present invention provides a biosensor for detecting allergies. The biosensor comprises a substrate; an electrode layer formed on a portion of an upper side of the substrate; a single-walled carbon nanotube layer formed on another portion of the upper side of the substrate; a linker layer formed on the single-walled carbon nanotube layer; and an antibody which is immobilized and bonded to the linker layer. In the biosensor for detecting allergies according to the present invention, 1-pyrenebutanoic acid succinimidyl ester is comprised as a linker on the substrate which is coated with a single-walled carbon nanotube, and the antibody which is capable of capturing an allergy-inducing protein is immobilized on the linker. When the allergy-inducing protein is captured by the antibody, a resistance value is changed, so that the allergy-inducing protein is capable of being detected with high sensitivity in a short time by sensing the changed resistance value.

Provided is a composite membrane for western blot, in which the composite membrane is prepared by combining nanofiber webs with nonwoven fabrics, and a basis weight of the nanofibers is in a range of 1 gsm to 50 gsm on the nonwoven fabrics, and an average pore size is in a range of 0.1 ?m to 1.0 ?m. The composite membrane for western blot including nanofibers has advantages such as saving of a production cost, and an excellent response characteristic due to a capillary phenomenon of a double structure, to thereby easily detect even a small amount of a particular substance present in a protein.

Provided is a composite membrane for western blot, in which the composite membrane is prepared by combining nanofiber webs with nonwoven fabrics, and a basis weight of the nanofibers is in a range of 1 gsm to 50 gsm on the nonwoven fabrics, and an average pore size is in a range of 0.1 ?m to 1.0 ?m. The composite membrane for western blot including nanofibers has advantages such as saving of a production cost, and an excellent response characteristic due to a capillary phenomenon of a double structure, to thereby easily detect even a small amount of a particular substance present in a protein.

The present invention relates to diagnostic test and technology. In particular, the present invention relates to a method for determining an analyte suspected to be present in a sample comprising contacting said sample with a sensor element comprising i) an anchor layer which is present on a solid support, ii) a first binding agent which is capable of specifically binding to the analyte, which is anchored in the anchor layer and which comprises at least one detectable label, and iii) a second binding agent which is capable of specifically binding to the analyte when bound to the first binding agent and which is immobilized on the solid support, for a time and under conditions which allow for specific binding of the analyte suspected to be present in the sample to the first binding agent and specific binding of the second binding agent to the analyte bound to the first binding agent and detecting the formation of the complex of first binding agent, analyte and second binding agent whereby the analyte is determined. Moreover, provided is a device for determining an analyte suspected to be present in a sample and the use thereof for determining an analyte suspected to be present in a sample in said sample. Moreover, the present invention contemplates a kit for determining an analyte suspected to be present in a sample.

The present invention relates to diagnostic test and technology. In particular, the present invention relates to a method for determining an analyte suspected to be present in a sample comprising contacting said sample with a sensor element comprising i) an anchor layer which is present on a solid support, ii) a first binding agent which is capable of specifically binding to the analyte, which is anchored in the anchor layer and which comprises at least one detectable label, and iii) a second binding agent which is capable of specifically binding to the analyte when bound to the first binding agent and which is immobilized on the solid support, for a time and under conditions which allow for specific binding of the analyte suspected to be present in the sample to the first binding agent and specific binding of the second binding agent to the analyte bound to the first binding agent and detecting the formation of the complex of first binding agent, analyte and second binding agent whereby the analyte is determined. Moreover, provided is a device for determining an analyte suspected to be present in a sample and the use thereof for determining an analyte suspected to be present in a sample in said sample. Moreover, the present invention contemplates a kit for determining an analyte suspected to be present in a sample.

Provided herein are macromolecular structures comprising charged macromolecules. In particular, provided herein are synthetic neutrophil extracellular traps and uses thereof.

Provided herein are macromolecular structures comprising charged macromolecules. In particular, provided herein are synthetic neutrophil extracellular traps and uses thereof.

An analyte indicator may include a porous base and may be included in an analyte sensor. The analyte indicator may retain its physical, chemical, and optical properties in the presence of compression. The porous base may not vary in opacity. The analyte indicator may include (i) a polymer unit attached or polymerized onto or out of the porous base and (ii) an analyte sensing element attached to the polymer unit or copolymerized with the polymer unit. The analyte sensing element may include one or more indicator molecule. The analyte sensing element may include one or more indicator polymer chains. The analyte indicator may include (i) an indicator polymer chain attached or polymerized onto or out of the porous base and (ii) indicator molecules attached to the indicator polymer chain.

An analyte indicator may include a porous base and may be included in an analyte sensor. The analyte indicator may retain its physical, chemical, and optical properties in the presence of compression. The porous base may not vary in opacity. The analyte indicator may include (i) a polymer unit attached or polymerized onto or out of the porous base and (ii) an analyte sensing element attached to the polymer unit or copolymerized with the polymer unit. The analyte sensing element may include one or more indicator molecule. The analyte sensing element may include one or more indicator polymer chains. The analyte indicator may include (i) an indicator polymer chain attached or polymerized onto or out of the porous base and (ii) indicator molecules attached to the indicator polymer chain.