A method for detecting an amount of an analyte in a solution includes providing an assay chamber including an electrode positioned at a first end of the assay chamber and a capture molecule attached to the electrode via a linker. A solution including an analyte, a binding partner of the analyte, at least one electrochemically active agent, and a detecting probe having a signaling tag attached thereto may be provided to the assay chamber. An electrical signal may be applied to the electrode to change the pH of the solution in the area near the electrode. The analyte may bind to the capture molecule and to the detecting probe at the first end of the assay chamber at the new pH. A signal produced by the signaling tag at the first end of the assay chamber may be measured to calculate the amount of the analyte in the solution.

Embodiments of the disclosure concern methods of determining the effectiveness of immune cells, such as T cells, with particular chimeric antigen receptors. In specific embodiments, a synapse between the CAR and the tumor antigen is measured for structure, signaling, and functionality by imaging. As such, the quality of the synapse is determined and positively correlates with effectiveness of the particular CAR immune cells.

Embodiments of the disclosure concern methods of determining the effectiveness of immune cells, such as T cells, with particular chimeric antigen receptors. In specific embodiments, a synapse between the CAR and the tumor antigen is measured for structure, signaling, and functionality by imaging. As such, the quality of the synapse is determined and positively correlates with effectiveness of the particular CAR immune cells.

A method of manufacturing a biopolymer optofluidic device including providing a biopolymer, processing the biopolymer to yield a biopolymer matrix solution, providing a substrate, casting the biopolymer matrix solution on the substrate, embedding a channel mold in the biopolymer matrix solution, drying the biopolymer matrix solution to solidify biopolymer optofluidic device, and extracting the embedded channel mold to provide a fluidic channel in the solidified biopolymer optofluidic device. In accordance with another aspect, an optofluidic device is provided that is made of a biopolymer and that has a channel therein for conveying fluid.

A method of manufacturing a biopolymer optofluidic device including providing a biopolymer, processing the biopolymer to yield a biopolymer matrix solution, providing a substrate, casting the biopolymer matrix solution on the substrate, embedding a channel mold in the biopolymer matrix solution, drying the biopolymer matrix solution to solidify biopolymer optofluidic device, and extracting the embedded channel mold to provide a fluidic channel in the solidified biopolymer optofluidic device. In accordance with another aspect, an optofluidic device is provided that is made of a biopolymer and that has a channel therein for conveying fluid.

An article that can be used for biomaterial capture comprises (a) a porous substrate; and (b) borne on the porous substrate, a polymer comprising interpolymerized units of at least one monomer consisting of (1) at least one monovalent ethylenically unsaturated group, (2) at least one monovalent ligand functional group selected from acidic groups, basic groups other than guanidino, and salts thereof, and (3) a multivalent spacer group that is directly bonded to the monovalent groups so as to link at least one ethylenically unsaturated group and at least one ligand functional group by a chain of at least six catenated atoms.

An article that can be used for biomaterial capture comprises (a) a porous substrate; and (b) borne on the porous substrate, a polymer comprising interpolymerized units of at least one monomer consisting of (1) at least one monovalent ethylenically unsaturated group, (2) at least one monovalent ligand functional group selected from acidic groups, basic groups other than guanidino, and salts thereof, and (3) a multivalent spacer group that is directly bonded to the monovalent groups so as to link at least one ethylenically unsaturated group and at least one ligand functional group by a chain of at least six catenated atoms.

The present invention relates to a peptide for detecting HE4 and use thereof and, more specifically, to a peptide for detecting HE4 that specifically binds to HE4 and use thereof for diagnosing ovarian cancer. The peptide that specifically binds to HE4, according to the present invention, can detect HE4 with high sensitivity and accuracy. In addition, when the peptide is expressed in the outer coat of a bacteriophage and constructed into a bacteriophage nano self-assembly, the peptide can conveniently detect HE4 from a sample through observation of a color change with a simple imaging device such as a smartphone, and in particular, the peptide can detect HE4 included in a small amount in saliva, and thus has the advantage of being very effectively used in early diagnosis of ovarian cancer.

The present invention relates to a peptide for detecting HE4 and use thereof and, more specifically, to a peptide for detecting HE4 that specifically binds to HE4 and use thereof for diagnosing ovarian cancer. The peptide that specifically binds to HE4, according to the present invention, can detect HE4 with high sensitivity and accuracy. In addition, when the peptide is expressed in the outer coat of a bacteriophage and constructed into a bacteriophage nano self-assembly, the peptide can conveniently detect HE4 from a sample through observation of a color change with a simple imaging device such as a smartphone, and in particular, the peptide can detect HE4 included in a small amount in saliva, and thus has the advantage of being very effectively used in early diagnosis of ovarian cancer.

A buoyancy enabled separation method for isolation from a sample including a variety of different cells a sparse subset of cells that is differentiated by a plurality of different cell surface markers. Microbubbles conjugated to antibodies are applied sequentially in a container with the previously used microbubbles disrupted prior to applying the next microbubbles conjugated to a different antibody. A system that includes a syringe-like container with a plunger and closeable opening. Further, a method for activating and expanding isolated T cells by applying antigen presenting microbubbbles having a flexible lipid shell mimicking an antigen presenting cell for generating immunological synapses.