An object of the present invention is to provide an immunochromatographic detection kit and detection method, in which continuous increasement of coloring intensity at a detecting portion for a long time is suppressed, capturing of a complex at a detecting portion is completed within a predetermined time, and increasement of coloring intensity is stopped, when a conjugate is mixed with a liquid sample and this mixture is supplied to a test strip. The present invention addresses the problem by retaining a free antibody against the analyte antigen and a saccharide on a sample pad, separately from antibodies of the conjugate and the detecting portion, in an immunochromatographic test strip. Further, the present invention addresses the problem by retaining a specific polymeric viscous substance on a sample pad in an immunochromatographic test strip.

An object of the present invention is to provide an immunochromatographic detection kit and detection method, in which continuous increasement of coloring intensity at a detecting portion for a long time is suppressed, capturing of a complex at a detecting portion is completed within a predetermined time, and increasement of coloring intensity is stopped, when a conjugate is mixed with a liquid sample and this mixture is supplied to a test strip. The present invention addresses the problem by retaining a free antibody against the analyte antigen and a saccharide on a sample pad, separately from antibodies of the conjugate and the detecting portion, in an immunochromatographic test strip. Further, the present invention addresses the problem by retaining a specific polymeric viscous substance on a sample pad in an immunochromatographic test strip.

The present invention relates to a composition comprising an ASM inhibitor as an active ingredient for preventing or treating degenerative neurological diseases. According to the present invention, when ASM is partially removed in an Alzheimer's disease model mouse, that is when ASM is inhibited therein, such when as an Alzheimer's disease model mouse with a partial removal of ASM is in a parabionic union with an Alzheimer's disease model mouse, or when an Alzheimer's disease model mouse is injected with the serum of an Alzheimer's disease model mouse from which ASM gene has been removed, the deposition of -amyloid in the brain tissue is inhibited and the ability to learn and remember are improved, and the present invention confirms such superb effects. Accordingly, ASM inhibitor can be effectively used to prevent or treat degenerative neurological diseases.

The present invention relates to a composition comprising an ASM inhibitor as an active ingredient for preventing or treating degenerative neurological diseases. According to the present invention, when ASM is partially removed in an Alzheimer's disease model mouse, that is when ASM is inhibited therein, such when as an Alzheimer's disease model mouse with a partial removal of ASM is in a parabionic union with an Alzheimer's disease model mouse, or when an Alzheimer's disease model mouse is injected with the serum of an Alzheimer's disease model mouse from which ASM gene has been removed, the deposition of -amyloid in the brain tissue is inhibited and the ability to learn and remember are improved, and the present invention confirms such superb effects. Accordingly, ASM inhibitor can be effectively used to prevent or treat degenerative neurological diseases.

The present invention relates to a method for treating degenerative neurological disorders in a subject in need thereof, comprising administering to a subject in need thereof a therapeutically effective amount of a composition comprising an acid sphingomyelinase (ASM) activity inhibitor or expression inhibitor as an active ingredient.

The present disclosure provides modified nucleosides, nucleotides, and nucleic acids, and methods of using thereof.

New approaches for selective detection of chemical agents such as sarin are necessary because of the high toxicity of sarin and related compounds, the potential of these compounds to be used as weapons of mass destruction, and the limitations of current detection methodologies. Herein is described an apparatus and a method for selective and amplified detection of sarin simulants deposited via an aerosol process. The simulant absorbs into a hydrogel, where it hydrolyzes upon contact with water producing elemental ions. The elemental ions are then concentrated via an ionic chemical potential gradient to a sensor, where it is detected. This technique has potential to amplify the capture efficiency of a sensor by a 1000-fold within couple of minutes.

A microfluidic device for determining whether an analyte is present in a sample is provided. The microfluidic device includes an elongated flow path having a polymeric medium, where the polymeric medium includes a first analyte detection domain having a first immobilized capture member that specifically binds to a first analyte and a second analyte detection domain having a second immobilized capture member that specifically binds to a second analyte. Also provided are methods, systems and kits in which the subject microfluidic devices find use, as well as methods of producing the same.

A microfluidic device for determining whether an analyte is present in a sample is provided. The microfluidic device includes an elongated flow path having a polymeric medium, where the polymeric medium includes a first analyte detection domain having a first immobilized capture member that specifically binds to a first analyte and a second analyte detection domain having a second immobilized capture member that specifically binds to a second analyte. Also provided are methods, systems and kits in which the subject microfluidic devices find use, as well as methods of producing the same.

Provided are compositions and methods for delivering biological moieties such as modified nucleic acids into cells to modulate protein expression. Such compositions and methods include the use of modified messenger RNAs, and are useful for production of proteins.