The present invention provides a system including: a protein having a domain that binds a membranal component; an inlet for sample flow, an Isotachophoresis (ITP) system and a flow generating means connected or coupled to the aqueous parts of the ITP. The invention also provides a method for detecting and or sorting cells with this system.

The invention is generally directed to modified filamentous fungal host cells comprising one or more nucleic acids encoding one or more polypeptides under the control of one or more promoters that are functional in said cells. Methods of using the modified cells to express one or more polypeptides are also disclosed, including methods of screening cells transformed with one or more expression vectors comprising nucleic acids derived from synthetic or genomic nucleic acids including, cDNAs. Methods of purifying one or more polypeptides or complexes comprising one or more polypeptides expressed in the modified cells, intended for use as substrates in structure/function studies, as therapeutic agents, as diagnostic reagents, or as human or animal vaccines, are also disclosed.

The invention is generally directed to modified filamentous fungal host cells comprising one or more nucleic acids encoding one or more polypeptides under the control of one or more promoters that are functional in said cells. Methods of using the modified cells to express one or more polypeptides are also disclosed, including methods of screening cells transformed with one or more expression vectors comprising nucleic acids derived from synthetic or genomic nucleic acids including, cDNAs. Methods of purifying one or more polypeptides or complexes comprising one or more polypeptides expressed in the modified cells, intended for use as substrates in structure/function studies, as therapeutic agents, as diagnostic reagents, or as human or animal vaccines, are also disclosed.

Methods for detecting His-tagged proteins using metal ion-chelating nitrilotriacetate (NTA) probes and polyacrylamide gel electrophoresis (PAGE) are disclosed. In one embodiment, the method includes using a metal ion-loaded NTA probe coupled to a UV-excitable fluorophore with visible emission and the presence of His-tagged proteins in the sample is determined by exposing the gel following PAGE, to a UV-light source with naked human eye or bench camera visualization. The metal ion-loaded NTA-containing chelator head can be coupled to a fluorophore that is not UV-excitable (i.e., with the majority of emission and excitation in the visible region of the electromagnetic spectrum. The method includes separating proteins in a sample using PAGE, contacting the gel following electrophoresis with a composition containing a metal ion-loaded NTA probe coupled to the fluorophore, to allow binding of the probe to the His-tagged proteins, and detecting the presence of the probe and therefore of the His-tagged proteins.

Methods for detecting His-tagged proteins using metal ion-chelating nitrilotriacetate (NTA) probes and polyacrylamide gel electrophoresis (PAGE) are disclosed. In one embodiment, the method includes using a metal ion-loaded NTA probe coupled to a UV-excitable fluorophore with visible emission and the presence of His-tagged proteins in the sample is determined by exposing the gel following PAGE, to a UV-light source with naked human eye or bench camera visualization. The metal ion-loaded NTA-containing chelator head can be coupled to a fluorophore that is not UV-excitable (i.e., with the majority of emission and excitation in the visible region of the electromagnetic spectrum. The method includes separating proteins in a sample using PAGE, contacting the gel following electrophoresis with a composition containing a metal ion-loaded NTA probe coupled to the fluorophore, to allow binding of the probe to the His-tagged proteins, and detecting the presence of the probe and therefore of the His-tagged proteins.

The present invention includes methods for detecting benign to malignant transformation of a cancer in a subject, comprising the steps of: collecting a sample from the subject prior to electrophoretic protein separation; activating electrophoretically separated ENOX2 transcript variants with an ENOX2 electron donor; and detecting the presence of the one or more activated ENOX2 transcript variants using a pan-ENOX2 detectable binding reagent, wherein the presence of one or more activated ENOX2 transcript variants in the sample is indicative of the malignant transformation of the cancer, whereby a 10 to 100 fold increase in detection sensitivity is obtained for the one or more activated ENOX2 transcript variants when compared to an equivalent non-activated ENOX2 transcript variant.

Disclosed are methods and apparatus for separation of biomolecules via two-dimensional gel electrophoresis, methods and apparatus for immunoblotting separated biomolecules, and methods for the use of biomolecules processed via the methods and apparatus of the present invention, including use in a clinical setting. The methods and apparatus for separation of biomolecules via two-dimensional gel comprises vertical agarose gel electrophoresis in the first dimension, and the electrophoresis of a novel non-denaturing 3-35% concave gradient polyacrylamide gel in the second dimension. This novel gel can be cast in a modified gel caster that can facilitate the pouring of multiple gels simultaneously. The methods and apparatus for immunoblotting are useful with any type of immunoblotting, including Western blot, Northern blot, and Southern blot analyses. These methods and apparatus provide safe, efficient and cost-effective immunoblots, while facilitating the reduction of exposure to toxic or radioactive materials, as well as the disposal of those materials.

Disclosed are methods and apparatus for separation of biomolecules via two-dimensional gel electrophoresis, methods and apparatus for immunoblotting separated biomolecules, and methods for the use of biomolecules processed via the methods and apparatus of the present invention, including use in a clinical setting. The methods and apparatus for separation of biomolecules via two-dimensional gel comprises vertical agarose gel electrophoresis in the first dimension, and the electrophoresis of a novel non-denaturing 3-35% concave gradient polyacrylamide gel in the second dimension. This novel gel can be cast in a modified gel caster that can facilitate the pouring of multiple gels simultaneously. The methods and apparatus for immunoblotting are useful with any type of immunoblotting, including Western blot, Northern blot, and Southern blot analyses. These methods and apparatus provide safe, efficient and cost-effective immunoblots, while facilitating the reduction of exposure to toxic or radioactive materials, as well as the disposal of those materials.

An electrophoretic device for detecting biomarkers in collected bodily fluid and methods of using the same.

An electrophoretic device for detecting biomarkers in collected bodily fluid and methods of using the same.