The invention relates to a system comprising superparamagnetic or anhysteretic nanoparticles (NPs) functionalised with an antibody, and a thin-film-type substrate of metal or an oxide thereof, functionalised with the same antibody; and to a method for detecting a biological analyte, such as a cell, protein, microorganism or similar, preferably a pathogenic microorganism, and even more preferably Listeria. The method comprises: (a) obtaining a control signal from a substrate (magnetic or not) coated with a thin film of metal or an oxide thereof, preferably gold, which can be functionalised with an antibody, the control signal being a magnetoresistance signal, a total magnetisation signal or a signal of the magnetisation curve; (b) mixing superparamagnetic or anhysteretic NPs functionalised with the antibody, with a liquid sample to analyse and confirm the presence or absence of the biological analyte, the NPs and the liquid sample making contact for 10-90 minutes; (c) dripping the dispersion obtained in step (b) onto the substrate of step (a), and then washing to remove NPs that are not chemically anchored to the surface of the biological analyte; (d) leaving the substrate to dry and re-measuring a signal in the same way as carried out in step (a); and (e) counteracting the control signal obtained in step (a) and the signal obtained in step (d), and in the absence of differences between the two measurements, confirming the absence of the biological analyte in the sample, the amount of microorganisms being directly proportional to the signal measured.

Methods and compositions are provided herein for treating and/or diagnosing ulcerative colitis in a subject, using one or more bacterial strains such as Bacillus sp. BT1B_CT2, Bacteroides coprocola M16, Bacteroides eggerthii CCUG 9559, Bacteroides vulgatus 8482, Butyricimonas virosa MT12, Coprococcus eutactus ATCC 27759, Desulfovibrio piger DSM 749, Dolosigranulum pigrum IFO 15550, Eubacterium biforme DSM 3989, Eubacterium eligens DSM 3376, Fusobacterium prausnitzii ATCC 27768, Howardella ureilytica GPC 589, Megasphaera elsdenii LC1, Parabacteroides johnsonii M-165, Phascolarctobacterium sp. YIT 12067, Roseburia hominus A2-183, or Ruminococcus callidus VPI 57-31.

Methods and compositions are provided herein for treating type 2 diabetes in a subject, using one or more bacterial strains such as Alistipes sp. HGB5, Atopobium parvulum type strain (IPP 1246), Bacteroides clarus DSM 22519, Butyrivibrio crossotus T9-40 A, Eubacterium hadrum B2-52, Prevotella stercorea CB35, Roseburia inulinivorans A2-194, Ruminococcus sp. 5.1.39BFAA, and Zinderia insecticola CARI.

Methods and compositions are provided herein for treating atopic dermatitis in a subject, using one or more bacterial strains such as Brevundimonas nasdae W1-2B, Capnocytophaga sputigena 4, Moraxella sp. LMG 5131, Neisseria elongata ATCC 29315, Staphylococcus felis GD521, Staphylococcus sciuri SC116, or Streptococcus intermedius 393.

This invention concerns compositions and methods of treating or diagnosing inflammatory disorders and other disorders, as well as compositions and methods of treating HIV.

A nasal irrigation diagnostic assembly comprising an irrigation device including a fluid collection portion structured to retain a biological sample, in the form of waste solution from the nasal cavity resulting from irrigation. A detection member disposed on said irrigation device is exposed to the biological sample and is structured to determine the existence of at least one analyte within the biological sample of the waste solution. The detection member comprises a plurality of detection zones individually structured to analyze the biological sample upon engagement therewith, wherein said plurality of zones include at least a reaction zone and a detection zone, which respectively include reagents cooperatively and collectively formulated to detect the existence of the at least one analyte within biological sample of the waste solution. A control zone may also be included to indicate the intended operability of at least the detection member.

The present invention provides a method for the detection of American foulbrood (AFB) in a beehive, said method comprising the steps of taking a sample from the beehive and testing the sample for a range of compounds, wherein the presence of one or more of the compounds in a relative amount of at least three times that of a non-infected hive indicates the presence of AFB in the beehive.

Disclosed herein are methods, compositions, kits, and systems for rapid multiplex detection of a microorganism of interest. Recombinant bacteriophages encoding capture moiety-indicator protein fusion products allow for the capture of indicator proteins on a surface. Cocktail compositions of recombinant bacteriophages can be used to detect potentially harmful bacteria. The specificity of recombinant bacteriophages for binding microorganisms allows targeted and highly specific detection of a microorganism of interest.

The invention is directed towards methods and compositions for identifying the specific microorganisms present in a particular potion of a water process system. The method involves obtaining a sample from the process and determining if a problematic organism exceeds an acceptable threshold. If so a remedial bio-control procedure can be applied long before any unwanted defects or problems occur.

A point-of-use kit is designed for optically detecting and quantifying bacterial endotoxin by employing specific formulations of Limulus amebocyte lysate (LAL), each formulation designed to optimize results with different sample classes. Kits are pre-certified for use with a variety of environmental, industrial, and clinical samples, each sample category having a unique kit design and containing a unique lysate reagent formulation. Pre-certification transfers time and reagent consuming tasks, such as assessment of sample compatibility and sample effect on reagent sensitivity to endotoxin from the user to the kit producer. A fixed dilution/sample treatment is employed, eliminating the need for a comparative water standard and for a sample positive control. The kit has LAL reagent prepackaged in dry polyethylene capped tubes, which retains reagent shelf life and optical clarity for accurate and reproducible results using a portable spectrophotometer/optical reader.