Superparamagnetic nanoparticle-based analytical method comprising providing a sample having analytes in a sample matrix, providing a point of care chip having analytical regions, each of which is a stationary phase having at least one or more sections, labeling each of the analytes with a superparamagnetic nanoparticle and immobilizing the labeled analytes in the stationary phase, providing an analytical device having a means for exciting the superparamagnetic nanoparticles in vitro and a means for sensing, receiving, and transmitting response of the excited superparamagnetic nanoparticles, placing the chip in the analytical device and exciting the superparamagnetic nanoparticles in vitro, sensing, receiving, and transmitting the response of the superparamagnetic nanoparticles, and analyzing the response and determining characteristic of the analytes, wherein the response of the superparamagnetic nanoparticles comprises harmonics. The present invention also provides the hybrid point of care chip and analyzer to be used in the analytical method.

A method for analyzing or detecting methimazole (“MTZ”) comprising contacting a sample suspected of containing MTZ with the dendrimer-stabilized silver nanoparticles and performing surface-enhanced Raman scattering (SERS). Graphene-dendrimer-stabilized silver nanoparticles (G-D-Ag).

The invention relates to a system comprising superparamagnetic or anhysteretic nanoparticles (NPs) functionalised with an antibody, and a thin-film-type substrate of metal or an oxide thereof, functionalised with the same antibody; and to a method for detecting a biological analyte, such as a cell, protein, microorganism or similar, preferably a pathogenic microorganism, and even more preferably Listeria. The method comprises: (a) obtaining a control signal from a substrate (magnetic or not) coated with a thin film of metal or an oxide thereof, preferably gold, which can be functionalised with an antibody, the control signal being a magnetoresistance signal, a total magnetisation signal or a signal of the magnetisation curve; (b) mixing superparamagnetic or anhysteretic NPs functionalised with the antibody, with a liquid sample to analyse and confirm the presence or absence of the biological analyte, the NPs and the liquid sample making contact for 10-90 minutes; (c) dripping the dispersion obtained in step (b) onto the substrate of step (a), and then washing to remove NPs that are not chemically anchored to the surface of the biological analyte; (d) leaving the substrate to dry and re-measuring a signal in the same way as carried out in step (a); and (e) counteracting the control signal obtained in step (a) and the signal obtained in step (d), and in the absence of differences between the two measurements, confirming the absence of the biological analyte in the sample, the amount of microorganisms being directly proportional to the signal measured.

Methods of preparing graphene/graphene oxide particulates under mild conditions, comprising reacting pristine graphene with hydrogen peroxide and a source of iron to oxidize the outer surface of the pristine graphene particulates in solution and yield graphene/graphene oxide particulates. Methods and articles incorporating the same are also disclosed.

A method for surface modification of nanoparticles includes the separate steps of removing ligands from the surface of the nanoparticles to form ligand-free nanoparticles, and mixing new ligands with the ligand-free nanoparticles to form modified nanoparticles.

A method for analyzing or detecting methimazole (“MTZ”) comprising contacting a sample suspected of containing MTZ with the dendrimer-stabilized silver nanoparticles and performing surface-enhanced Raman scattering (SERS). Graphene-dendrimer-stabilized silver nanoparticles (G-D-Ag).

Single atom nanozymes and associated immunoassays, method of making, and method of using such immunoassays are described herein. For example, a method of making a single atom nanozyme includes forming a soft template having multiple nanoscale structures in an aqueous solution and adding a monomer and a metal containing salt into the aqueous solution. The metal containing salt causes polymerization of the monomer to form multiple nanostructures according to the nanoscale structures of the soft template. The method also includes coating the individual formed nanostructures with a confinement layer in the aqueous solution before pyrolyzing. During pyrolysis, the confinement layer at least restricts or completely prevents migration of atoms on the external surface of the individual nanostructures.

A time-resolved fluorescence and chromogenic dual-signal test strip for estrogen is based on the principle of immune recognition and fluorescence resonance energy transfer. In time-resolved fluorescence mode, estrogen-BSA-persistent luminescence particle complex is the fluorescence donor, and colloidal gold modified with estrogen monoclonal antibody is the fluorescence receptor, which is also chromogenic signal unit in the chromogenic mode. The photos of strips in both modes are obtained with smart phones. The time-resolved fluorescence intensity of the test strip test zone is positively correlated with estrogen content, and the chromogenic intensity is negatively correlated with estrogen content. The competitive time-resolved fluorescence and chromogenic dual-signal immunochromatographic test strips can accurately and quickly detect estrogen and estrogen-like compounds.

Described herein are methods such as multi-omic methods for assessing a disease such as cancer. The multi-omic methods may integrate proteomic, transcriptomic, genomic, lipidomic, or metabolomic data. The method screening diseases or disease states. Also described herein are methods for screening for diseases or disease states from biological samples. The methods may include assessing whether a nodule, mass, or cyst is cancerous.

The present invention relates to a method for detecting an antibody-drug-conjugate and relates to a method for determining the efficacy of an antibody-drug-conjugate with high accuracy by a quantitative technique for identifying an expression level of a target molecule in a target cell of the antibody-drug-conjugate and interactions therebetween. According to the method, visualizing a drug and an antibody, or components of an antibody-drug-conjugate, by immunostaining with a phosphor integrated dot enables detection of the antibody-drug-conjugate and the components.