Provided herein is a wet protein blotting system comprising (a) a dry assembly module; (b) a protein blotting chamber; (c) a liquid transfer buffer handling system; and (d) a control panel and power supply. The wet protein blotting systems provided herein reduces the blotting time of a traditional wet blotting process while maintaining the ability for high sensitivity in the transfer of proteins from a polyacrylamide gel to a membrane. Also provided are dry assembly modules and liquid transfer buffer handling systems that enable a fully automatic buffer manipulation system by one or more than one transfer buffers could be pumped into the protein blotting chamber to enhance the transfer efficiency. Also provided is a protein blotting chamber capable of controlling the temperature of the wet protein blotting system.

A blotter assembly for use in electrophoresis comprising a block and a closed cell silicone blotter pad adhesively attached thereto and restricted against accidental movement relative thereto.

Antibiotics (Abx) are the worlds most misused drugs. Antibiotics misuse occurs when the drug is administered in case of a non-bacterial infection (such as a viral infection) for which it is ineffective. Overall, it is estimated that 40-70% of the worldwide Abx courses are mis-prescribed. The financial and health consequences of Abx over-prescription include the direct cost of the drugs, as well as the indirect costs of their side effects, which are estimated at >$15 billion annually. Furthermore, over-prescription directly causes the emergence of Abx-resistant strains of bacteria, which are recognized as one of the major threats to public health today. This generates an immediate need for reliable diagnostics to assist physicians in correct Abx prescription, especially at the point-of-care (POC) where most Abx are prescribed. Accordingly, some aspects of the present invention provide methods using biomarkers for rapidly detecting the source of infection and administrating the appropriate treatment.

The present disclosure provides systems for gel electrophoresis and electrotransfer comprising one or more chambers that can removably and interchangeably receive either an electrophoresis cassette, or an electrotransfer cassette, and provides an electrical interface for both electrophoresis and electrotransfer of biomolecules. The present disclosure also provides electrophoresis devices including clamps and electrotransfer cassettes and related devices. Methods for electrophoresis and electrotransfer using the systems and devices of the disclosure are also provided.

Systems and methods for automatically identifying and characterizing one or more lanes in image data for one or more electrophoresed samples. The method includes receiving data representing an image of one or more electrophoresed samples, segmenting the data into one or multiple data segments or portions within a region of interest (ROI), wherein the one or multiple data segments represent (e.g., when visually displayed) one or multiple lane segments along a first axis in the ROI, each of the one or multiple lane segments traversing one or multiple lanes in the image data, generating an intensity profile for at least a first data segment of the one or multiple data segments along a second axis orthogonal to the first axis, and processing the intensity profile to determine a location and parameters for each of one or multiple lanes in the first data segment.

A method of collecting one or more target macromolecules in a capture membrane by gel electrophoresis is disclosed, as well as a kit for macromolecule isolation and recovery including: a preformed gel; a capture device; an insertion guide; and optionally, a migration gauge.

Systems and methods for identifying DNA strand size and purifying the DNA based on strand size using electrophoresis. The methods include moving, via voltage, a plurality of DNA strands through a separation gel from an inlet of a capillary or passage to either a first outlet or a second outlet dependent on the DNA strand length. In some implementations, the system is a capillary electrophoresis system. In other implementations, the system is a microfluidic lab-on-a-chip.

The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.

The present disclosure provides devices and systems for gel electrophoresis and/or electrotransfer and/or detection of biomolecules. Devices for electrotransfer comprise: one or more receptacles comprising: a biomolecule receiving material (BMR); a first and second electrode; wherein the second electrode is located after and/or distal to an area where biomolecules are received OR wherein both electrodes are arranged to allow current flow in at least two directions, wherein the second direction of current flow is along the plane of the BMR. Devices and systems can be adapted to perform electrophoresis, wherein the receptacle further comprises a matrix for electrophoretic separation of biomolecules. Devices and systems can be further adapted to perform detection, wherein the receptacle further comprises reagent dispensing channels or ports and/or reagents to detect electrotransferred biomolecules. Devices and systems of the disclosure can be microfluidic devices. Methods using the systems and devices of the disclosure are also provided.

An electrophoresis device is used to separate a biological substance using a separation medium. A recovery hole configured to recover the separated target biological substance is formed in an electrophoresis device, the recovery hole is configured to be capable of accommodating a solvent that can suspend the target biological substance, a buffer tank configured to accommodate a buffer solution is provided below the recovery hole, and a membrane is disposed between the recovery hole and the buffer tank.