A method for preparing for preparing a solution comprising a plurality of particles in a vial is disclosed. The method includes agitating the vial at a first predetermined mixing speed. The method also includes dispersing, into the vial, during the agitating, a first volume of buffer solution at a first predetermined dispensing rate to suspend the plurality of particles in the solution, wherein particles of the plurality of particles comprise a unique identifying feature.

The present disclosure relates to a system, method, and kit for particle detection and analysis. Devices disclosed herein may include at least an optical source, a fludic chip containing a multiplex bead array, and a detection module, wherein the sample flows within the fludic chip past a detection window, where the cells or particles are imaged by an image acquisition and analysis module that may include an optical detector. The image acquisition and analysis module counts the labeled particles and software allows for analysis of bead population.

Disclosed is an analysis method including: moving a complex, by means of a magnet unit, in a flow path of a specimen cartridge which includes the flow path and a detection vessel, the complex containing a test substance formed on magnetic particles and a labeled substance binding to the test substance; taking an image of the magnetic particles in the specimen cartridge; and measuring a label of the labeled substance contained in the complex in the detection vessel.

Disclosed is an analysis method for detecting and analyzing light from a sample prepared so as to emit light in accordance with an amount of a test substance, the analysis method including taking an image of a storage member configured to store the sample therein; switching a state of a reflector to a state in which light from the sample is reflected toward a light detection unit and detecting light from the sample by the light detection unit; and outputting an analysis result of the sample on the basis of a light amount detected by the light detection unit.

A biosensor device for detecting biological particles, the biosensor device comprising a substrate, a regular pattern of pores formed in the substrate, and a plurality of sensor active structures each of which being arranged on a surface of a corresponding one of the pores, wherein each of the plurality of sensor active structures is sensitive to specific biological particles and is adapted to modify electromagnetic radiation interaction properties in the event of the presence of the respective biological particles.

Sample analysis systems and methods using assay surfaces, assay processing units (APUs), assay processing systems (APSs), and laboratory systems are disclosed. An assay surface includes a sample processing component comprising a plurality of regions, including at least one wash region and at least one storage region configured to hold a plurality of solid supports moveable through the regions under a magnetic force, and a detection component configured to receive the solid supports. An APU includes an assay surface receiving component, a magnetic element configured to generate a moveable magnetic field, and one or more processors configured to move the magnetic field. An APS includes one or more assay surfaces and an APU. A laboratory system includes one or more APSs and a controller for parallel processing. Sample processing and detection methods are disclosed with a reduced sample volume and/or shortened processing time and/or higher sensitivity.

The invention relates to a method for detecting organisms in a liquid sample, comprising the provision of a capture area including particles in a liquid medium and subjected to a hydrodynamic flow, wherein the organisms to be detected are capable of binding to these particles, the method comprising the steps of: (a) circulating the sample through the capture area; (b) circulating a growth medium through the capture area; and (c) determining the presence, nature or concentration of organisms in the capture area; said particles being retained in the capture area as a fluidized bed for at least one part of these steps.

The invention also relates to a system for implementing this method.

In one aspect, presence and/or level of an analyte within a sample is determined by use of a construct comprising a magnetic moiety and a fluorescent moiety. In one embodiment, the construct is magnetically migrated to a transparent surface and then dragged along the surface. In one aspect, an evanescent field is applied and changes in the diffusional or rotational properties of the fluorescent moiety as it migrates in and out of the evanescent field are measured by changes in its fluorescent emission, providing a measure of the interaction between the construct and a component of the sample.

Disclosed herein is an improved method for magnetic capture of target molecules (e.g., microbes) in a fluid. Kits and solid substrates for carrying the method described herein are also provided. In some embodiments, the methods, kits, and solid substrates described herein are optimized for separation and/or detection of microbes and microbe-associated molecular pattern (MAMP) (including, e.g., but not limited to, a cell component of microbes, lipopolysaccharides (LPS), and/or endotoxin).

The present disclosure provides a system comprising a communication interface and computer for assigning a label to the biomolecule fingerprint, wherein the label corresponds to a biological state. The present disclosure also provides a sensor arrays for detecting biomolecules and methods of use. In some embodiments, the sensor arrays are capable of determining a disease state in a subject.