Kits and methods for the detection and enrichment of RNA viruses of the family Coronaviridae. The detection method comprises the steps of (a) coupling a binding agent that specifically recognizes and binds to a virus component to a carrier material, (b) incubating the carrier material with the thereon coupled binding agent with a virus-containing sample, (c) staining the viruses immobilised on the carrier material with a staining agent, and (d) detecting stained virus particles via a physical, chemical or biological detection means. The methods may be suitable for the rapid and efficient detection of coronaviruses, such as SARS-CoV-2. With the methods and kits, it is possible to perform rapid high-throughput tests in a large population. At the same time, the enrichment procedure makes it possible to enrich viral samples, e.g. from a throat swab of a patient, for use in a subsequent PCR.

Provided herein are methods of assessing or determining the presence or absence of particles, such as bead particles, present in a cell composition. Also provided are articles of manufacture and kits for use in the methods.

The present disclosure relates to a Proteomics-to-Genomics approach allows for in silico validation of biomarkers and drug targets. Biomarkers having high prognostic value in predicting cancer patient populations that may benefit from mitochondrial biogenesis inhibitor therapy may be identified under the present approach. Also disclosed are methods for identifying candidates for anti-mitochondrial therapy, and in particular mitochondrial biogenesis inhibitor therapy. Diagnostic kits including reagents for determining transcripts or probes of high prognostic value are also disclosed. Additionally, mitochondrial biogenesis inhibitors may be used as anti-cancer agents for diverse oncogenic stimuli, including for example, c-MYC and H-Ras oncogenes, as well as environmental stimuli such as, for example rotenone.

An electrochemiluminescence method of detecting an analyte in a liquid sample and a corresponding analysis system. An analyte in a liquid sample is detected by first providing a receptacle containing a fluid comprising protein coated magnetic microparticles to a stirring unit. Stirring of the fluid is necessary since the density of the microparticles is usually higher than the density of the buffer fluid. Thus the microparticles tend to deposit on the bottom of the receptacle leading to an aggregation of the microparticles because of weak interactions. To obtain representative measurements a homogeneous distribution of the microparticles in the buffer fluid is necessary to ensure a constant concentration of microparticles for each analysis cycle. It is further necessary to provide disaggregation of the microparticles, which is also realized by stirring the fluid. Stirring is conducted with a rotational frequency that is adapted to the amount of fluid to be stirred.

An apparatus configured to receive particles in a liquid includes: a housing comprising a housing inlet and a housing outlet; and a mesh located in the housing between the housing inlet and the housing outlet, the mesh having spaces greater than a greatest transverse dimension of the particles. The apparatus operates to break up agglomerates of the particles, such as agglomerates of magnetic particles. Other systems and methods of receiving and transferring liquids containing particles having a propensity to agglomerate are disclosed, as are other aspects.

Devices, systems, and their methods of use, for sorting or separating magnetic particles are provided. A magnetic source, e.g., with at least one sharp feature, can be employed to exert a strong magnetic field on magnetic particles in order to separate particles of a desired and predictable property.

This invention relates to a method of dispersing magnetic particles, said method comprising or consisting of: (a) in a vessel, combining at least one permanent magnet and said magnetic particles in a liquid phase; and (b) triggering a fluctuating or oscillating motion of said permanent magnet using a magnetic field; thereby dispersing said particles.

The disclosure relates to the technical field of virus detection, in particular, to an IgA antibody and a kit capable of specifically recognizing RBD protein. The antibody can be used as a calibrator of IgA antibody against the RBD protein of SARS-CoV-2. the disclosure further relates to a kit comprising the antibody which enables automated, high-throughput, and rapid detection of IgA antibody against novel coronavirus pneumonia

A detection method includes forming a complex by binding a target substance and a dielectric particle modified by a single-domain antibody that is bindable to the target substance, separating the complex and an unbound particle in a fluid with dielectrophoresis, the unbound particle being the dielectric particle not forming the complex, and detecting the target substance contained in the separated complex with an imaging element.

The present invention relates to methods and products for isolating nucleic acids from samples containing biological material. In particular, the present invention relates to silica-coated magnetic particles, processes for their preparation and their use in methods of isolating nucleic acids samples containing biological material.