A system for assaying a biological sample for a presence of a target analyte includes an assaying device and a computer controller. The assaying device includes a housing, a receptacle disposed in the housing, and a source of activation energy. The receptacle is configured to accept an electrophoresis cell. The electrophoresis cell has a recess area configured to accept a chip configured to accept the biological sample. The chip includes a polymeric separation medium with activatable functional groups that covalently bond to the target analyte when activated. The source of activation energy is configured to supply activation energy to activate the activatable functional groups. The computer controller is operably coupled to the source of activation energy and is configured to activate the source of activation energy to direct an application of activation energy to the polymeric separation medium to activate the activatable functional groups.

A multiple capillary florescent detection system employing optical fiber bundles that each fiber bundle has more than one fiber illuminating each sample vessel.

An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided.

A kit is disclosed for distinguishing between bacterial and viral infections. One of the antibodies of the kit specifically binds to TNF-related apoptosis-inducing ligand (TRAIL) protein and another of the antibodies of the kit specifically binds to Procalcitonin (PCT) protein.

A method of capillary electrophoresis is provided for binder selection. In an embodiment, a capillary electrophoresis method comprises selecting an electroosmotic flow (EOF) in a capillary such that at least one target-binder (TB) complex has a target-binder velocity vector (vTB) co-directed with an electric field vector (E) and at least one non-binder (N) has a non-binder velocity vector (vN) in the opposite direction to the electric field vector (E); introducing a sample comprising the at least one target-binder (TB) complex, the at least one non-binder (N), and at least one running buffer into a capillary inlet of the capillary; applying an electric field directed from the capillary inlet to a capillary outlet of the capillary to separate the at least one target-binder (TB) complex from the at least one non-binder (N); and detecting the at least one target-binder complex.

A method for glycan profiling by capillary electrophoresis (CE), and a CE system for glycan analysis (N-Glycan). The CE system uses integrated dual optical fibers for both radiation excitation and emission detection. The CE system is configured for performing a two-color detection for data analysis. A single radiation excitation source is used to excite two emission fluorophores or dyes in the sample solution to be analyzed. One emission dye is to tag the sample and the other dye is used to provide a reference marker (e.g., a Dextran Ladder) for the sample run. Two detectors (e.g., photomultipler tubes (PMTs)) are applied to simultaneously detect the fluorescent emissions from the dyes. The data collected by both detectors are correlated (e.g., synchronized, and/or super-positioned for analysis) for accurate data peak identification.

In a nanopore sensor, a nanopore disposed in a support structure has a nanopore diameter and nanopore resistance, R.sub.Pore. A fluidic passage, disposed in fluidic connection between a first fluidic reservoir and the nanopore, has a cross-sectional extent, along at least a portion of the fluidic passage length, that is greater than the diameter of the nanopore and that is less than the fluidic passage length. The fluidic passage has a fluidic passage resistance, R.sub.FP, of at least about 10% of the nanopore resistance, R.sub.Pore, and no more than about 10 times the nanopore resistance, R.sub.Pore. The nanopore is disposed in fluidic connection between the fluidic passage and a second fluidic reservoir. At least one electrical transduction element is disposed at the fluidic passage and electrically connected to produce an indication of electrical potential local to the fluidic passage.

The present disclosure generally relates to systems comprising devices for effecting epitachophoresis and sample detection and methods of using such systems. Epitachophoresis may be used to effect sample analysis, such as by selective separation, detection, extraction, and/or pre-concentration of target analytes such as, for example, DNA, RNA, and/or other biological molecules. Sample detection may be used to trigger automated target analyte collection. Said target analytes may be used for desired downstream applications and further analysis.

Methods for detecting His-tagged proteins using metal ion-chelating nitrilotriacetate (NTA) probes and polyacrylamide gel electrophoresis (PAGE) are disclosed. In one embodiment, the method includes using a metal ion-loaded NTA probe coupled to a UV-excitable fluorophore with visible emission and the presence of His-tagged proteins in the sample is determined by exposing the gel following PAGE, to a UV-light source with naked human eye or bench camera visualization. The metal ion-loaded NTA-containing chelator head can be coupled to a fluorophore that is not UV-excitable (i.e., with the majority of emission and excitation in the visible region of the electromagnetic spectrum. The method includes separating proteins in a sample using PAGE, contacting the gel following electrophoresis with a composition containing a metal ion-loaded NTA probe coupled to the fluorophore, to allow binding of the probe to the His-tagged proteins, and detecting the presence of the probe and therefore of the His-tagged proteins.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.